tannin
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Strategies are sought to reduce intestinal colonisation of food-producing animals by Campylobacter jejuni, a leading bacterial cause of human foodborne illness worldwide. Presently, we tested the antimicrobial activity of hydrolysable-rich blackberry, cranberry and chestnut tannin extracts and condensed tannin-rich mimosa, quebracho and sorghum tannins (each at 100 mg/mL) against C. jejuni via disc diffusion assay in the presence of supplemental casamino acids. We found that when compared to non-tannin-treated controls, all tested tannins inhibited the growth of C. jejuni and that inhibition by the condensed tannin-rich mimosa and quebracho extracts was mitigated in nutrient-limited medium supplemented with casamino acids. When tested in broth culture, both chestnut and mimosa extracts inhibited growth of C. jejuni and this inhibition was much greater in nutrient-limited than in full-strength medium. Consistent with observations from the disc diffusion assay, the inhibitory activity of the condensed tannin-rich mimosa extracts but not the hydrolysable tannin-rich chestnut extracts was mitigated by casamino acid supplementation to the nutrient-limited medium, likely because the added amino acids saturated the binding potential of the condensed tannins. These results demonstrate the antimicrobial activity of various hydrolysable and condensed tannin-rich extracts against C. jejuni and reveal that condensed tannins may be less efficient than hydrolysable tannins in controlling C. jejuni in gut environments containing high concentrations of amino acids and soluble proteins.
- MeSH
- Aesculus chemie MeSH
- antibakteriální látky farmakologie MeSH
- Campylobacter jejuni účinky léků růst a vývoj MeSH
- diskové difúzní antimikrobiální testy MeSH
- hydrolyzovatelné taniny farmakologie MeSH
- kampylobakterové infekce mikrobiologie MeSH
- lidé MeSH
- proantokyanidiny farmakologie MeSH
- Rosaceae chemie MeSH
- rostlinné extrakty farmakologie MeSH
- Vaccinium macrocarpon chemie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
Polyfenolické obsahové látky - triesloviny (taníny) a flavonoidy sú hlavné biologicky účinné sekundárne metabolity hamamelu virgínskeho. Cieľom práce bolo stanovenie ich obsahu v droge - listoch spolu s konármi z troch vegetačných období zberu za použitia spektrofotometrických metód. Z výsledkov stanovenia vyplynulo, že obsah trieslovín zo zberu v máji bol nižší, než zo zberu drogy v septembri, resp. októbri. Naopak obsah flavonoidov zo zberu v máji bol vyšší ako zo zberu v septembri, resp. v októbri v tom istom roku.
Polyphenolic constituents - tannins and flavonoids - are the principal biologically active secondary metabolites of Hamamelis virginiana L. The paper aims to determine their content in the drug - leaves and branches from three vegetation periods of collection with the use of spectrophotometric methods. The results of determination show that the content of tannins from the collection in May was lower than from the collection of the drug in September, or October. On the other hand, the content of flavonoids from the collection in May was higher than from the collection in September, or October, in the same year.
