- MeSH
- Anti-Infective Agents therapeutic use MeSH
- Crohn Disease drug therapy surgery complications MeSH
- Adult MeSH
- Humans MeSH
- Peritonitis * drug therapy surgery MeSH
- Tigecycline * pharmacology therapeutic use MeSH
- Check Tag
- Adult MeSH
- Humans MeSH
- Female MeSH
- Publication type
- Case Reports MeSH
- MeSH
- Drug Resistance, Bacterial * MeSH
- Congresses as Topic MeSH
- Parasitic Diseases * MeSH
- Vaccination * MeSH
- Virus Diseases * MeSH
- Publication type
- News MeSH
- MeSH
- Anti-Bacterial Agents pharmacology therapeutic use MeSH
- Bronchopneumonia * diagnosis drug therapy MeSH
- Diagnostic Imaging utilization MeSH
- Discitis * diagnosis drug therapy complications MeSH
- Gentamicins pharmacology therapeutic use MeSH
- Imipenem pharmacology therapeutic use MeSH
- Drug Therapy, Combination MeSH
- Middle Aged MeSH
- Humans MeSH
- Microbiological Techniques MeSH
- Respiratory Insufficiency MeSH
- Rifampin pharmacology therapeutic use MeSH
- Staphylococcus aureus drug effects MeSH
- Respiration, Artificial MeSH
- Vancomycin pharmacology therapeutic use MeSH
- Voriconazole pharmacology therapeutic use MeSH
- Treatment Outcome MeSH
- Check Tag
- Middle Aged MeSH
- Humans MeSH
- Female MeSH
- Publication type
- Case Reports MeSH
Cíl: Detekovat přítomnost toxinů A a B Clostridium difficile ve stolici pacientů hospitalizovaných ve Fakultní nemocnici Olomouc (FNOL), u kterých se objevily průjmy nebo i jiná břišní symptomatologie (bolesti břicha, meteorismus, porucha trávení, subileózní stav) v souvislosti s antibioterapií. Vzhledem k občasnému výskytu dyspeptických potíží i nekrotizujících enterokolitid u nedonošených novorozenců zvážit kromě nezralosti střeva i možnost spoluúčasti těchto toxinů na rozvoji onemocnění. Pro potvrzení hypotézy vyšetřit stoUce na průkaz toxinů i u fyziologických nebo patologických novorozenců bez gastrointestinální symptomatologie. U vybraných vzorků stolic porovnat detekci toxinů s kultivačním průkazem kmenů C. difficile.
To detect the presence of Clostridium difficile toxins A and B in the stools of patients hospitalized in the University Hospital Olomouc who developed diarrhoea or other abdominal symptoms (abdominal pain, tympanites, indigestion, partial intestinal obstruction) related to antibiotic therapy. Given occasional dyspepsia and necrotizing enterocolitis in preterm neonates, to consider the potential role of these toxins, in addition to that of the immature intestine, in the development of the condition. To confirm the hypothesis by stool tests to detect the toxins also in normal neonates or those with no gastrointestinal symptoms. In selected stool samples, to compare detection of the toxins with C. difficile strains confirmed by culture.
- MeSH
- Anti-Bacterial Agents therapeutic use MeSH
- Clostridioides difficile immunology pathogenicity MeSH
- Child MeSH
- Financing, Organized MeSH
- Clostridium Infections diagnosis complications therapy MeSH
- Humans MeSH
- Infant, Newborn, Diseases etiology immunology therapy MeSH
- Infant, Premature immunology MeSH
- Infant, Newborn immunology MeSH
- Enterocolitis, Pseudomembranous diagnosis etiology therapy MeSH
- Check Tag
- Child MeSH
- Humans MeSH
- Infant, Newborn immunology MeSH
- Publication type
- Abstracts MeSH
The presented study aims at analyzing an increasing prevalence of vancomycin-resistant enterococci (VRE) isolated from various kinds of clinical material obtained from patients in the Department of Hemato-oncology (DHO), University Hospital in Olomouc, Czech Republic. Between January 1 and March 31, 2005, enterococci were isolated by standard microbiological procedures using both clinical material obtained from hospitalized patients and samples from the department environment. Resistance to vancomycin and teicoplanin was determined by a standardized microdilution method. Phenotype determination of resistance to vancomycin was verified by PCR detection of vanA and vanB genes. In VanA Enterococcus faecium, macrorestriction analysis was performed by pulsed-field gel electrophoresis. During the monitored period, a total of 128 Enterococcus sp. strains were isolated, of which 38 (30 %) isolates from 22 different patients were determined as VRE. Dominating were Enterococcus faecium VanA (63 %) and Enterococcus casseliflavus VanC (16 %) strains. At the same time, one Enterococcus faecium VanA strain was acquired from a bed-side table used by a patient in whom a similar strain had been isolated repeatedly from various clinical materials including a rectal swab taken in 2004. Based on the macrorestriction analysis of genome DNA in 24 vancomycin-resistant Enterococcus faecium VanA strains isolated from the patients' clinical material, one strain from the bed-side table surface and one strain isolated from stools in 2004, 8 unique restriction profiles with similarity ranging from 90 % to 100 % were identified, which could be classified into 3 clonal types. Thus, we can assume not only the endogenous origin of the VRE in hemato-oncological patients and their potential selection caused by therapy with broad-spectrum antibiotics but also the ability of the strains to survive in a hospital setting and, subsequently, to be spread clonally by various vectors.
- MeSH
- Drug Resistance, Bacterial MeSH
- Enterococcus classification drug effects isolation & purification MeSH
- Gram-Positive Bacterial Infections transmission MeSH
- Hematologic Neoplasms microbiology MeSH
- Cross Infection microbiology transmission MeSH
- Humans MeSH
- Vancomycin Resistance MeSH
- Teicoplanin pharmacology MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Práce popisuje kazuistiku oroglandulární formy tularémie se současným záchytem Nocardia sp. v punktátu z uzliny. Shrnuje klinický průběh, problémy se stanovením etiologického agens (sérologický průkaz tularémie a kultivační záchyt nokardií). Zmiňuje terapii kombinací doxycyklinu se streptomycinem a následně cotrimoxazolem. Diskutuje otázku vzniku onemocnění a zvažuje současnou či sekundární infekci nokardiemi.
- MeSH
- Child MeSH
- Drug Therapy methods MeSH
- Francisella tularensis pathogenicity MeSH
- Clinical Laboratory Techniques statistics & numerical data MeSH
- Cells, Cultured methods MeSH
- Humans MeSH
- Nocardia Infections diagnosis etiology drug therapy MeSH
- Tularemia diagnosis etiology drug therapy MeSH
- Check Tag
- Child MeSH
- Humans MeSH
- Male MeSH
- Publication type
- Case Reports MeSH
- Review MeSH
