In this study, we investigate the preparation of surface pattern of functional groups on poly(lactide) (PLA) surfaces through the controlled deposition of core-shell self-assemblies based on functionalized PLA-b-PEO amphiphilic block copolymers from selective solvents. Through grafting RGDS peptide onto the functionalized copolymer surface, the presented approach enables to prepare PLA surfaces with random and clustered spatial distribution of adhesive motifs. The proposed topography of the adhesion motif was proved by atomic force microscopy techniques using biotin-tagged RGDS peptide grafted on the surface and streptavidin-modified gold nanospheres which bind the tagged RGDS peptides as a contrast agent. The cell culture study under static and dynamic conditions with MG63 osteosarcoma cell line showed that the clustered distribution of RGDS peptides provided more efficient initial cell attachment and spreading, and resistance to cell detachment under dynamic culture compared to randomly distributed RGDS motif when with the same average RGDS peptide concentration.
- MeSH
- biomimetika MeSH
- buněčná adheze účinky léků MeSH
- kovové nanočástice MeSH
- laktáty chemie MeSH
- lidé MeSH
- mikroskopie atomárních sil MeSH
- nádorové buněčné linie MeSH
- nanostruktury chemie MeSH
- oligopeptidy MeSH
- polyethylenglykoly chemie MeSH
- povrchové vlastnosti MeSH
- streptavidin chemie MeSH
- vazba proteinů MeSH
- zlato MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Autologous vein grafts are often used for treating damaged vessels, e.g. arteriovenous fistulas or arterial bypass conduits. Veins have a different histological structure from arteries, which often leads to intimal hyperplasia and graft restenosis. The aim of this study was to develop a perivascular sirolimus-delivery system that would release the antiproliferative drug sirolimus in a controlled manner. Polyester Mesh I was coated with purasorb, i.e. a copolymer of L-lactide and ɛ-caprolactone, with dissolved sirolimus; Mesh II was coated with two copolymer layers; the layer with dissolved sirolimus was overlaid with pure purasorb. This arrangement allowed sirolimus to be released for 6 and 4 weeks, for Mesh I and Mesh II, respectively. Mesh II released sirolimus more homogeneously, without the initial burst effect during the first week. However, the cumulative release curve was steeper at later time points than the curve for Mesh I. Both meshes inhibited proliferation of rat vascular smooth muscle cells during 14-day culture in vitro and preserved excellent cell viability. Newly developed sirolimus-releasing perivascular meshes are promising devices for preventing autologous graft restenosis.
- MeSH
- biokompatibilní potahované materiály MeSH
- farmaceutická chemie MeSH
- farmaceutická technologie metody MeSH
- kardiovaskulární látky aplikace a dávkování chemie farmakologie MeSH
- kinetika MeSH
- krysa rodu rattus MeSH
- kultivované buňky MeSH
- léky s prodlouženým účinkem MeSH
- mikroskopie elektronová rastrovací MeSH
- molekulová hmotnost MeSH
- myocyty hladké svaloviny účinky léků MeSH
- nosiče léků MeSH
- okluze cévního štěpu prevence a kontrola MeSH
- polyestery chemie MeSH
- potkani Wistar MeSH
- povrchové vlastnosti MeSH
- příprava léků MeSH
- proliferace buněk účinky léků MeSH
- rozpustnost MeSH
- sirolimus aplikace a dávkování chemie farmakologie MeSH
- stabilita léku MeSH
- svaly hladké cévní účinky léků MeSH
- vysokoúčinná kapalinová chromatografie MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
The surface of poly(L-lactide) (PLLA) films deposited on glass coverslips was modified with poly(DL-lactide) (PDLLA), or 1:4 mixtures of PDLLA and PDLLA-b-PEO block copolymers, in which either none, 5% or 20% of the copolymer molecules carried a synthetic extracellular matrix-derived ligand for integrin adhesion receptors, the GRGDSG oligopeptide, attached to the end of the PEO chain. The materials, perspective for vascular tissue engineering, were seeded with rat aortic smooth muscle cells (11,000 cells/cm(2)) and the adhesion, spreading, DNA synthesis and proliferation of these cells was followed on inert and bioactive surfaces. In 24-h-old cultures in serum-supplemented media, the number of cells adhering to the PDLLA-b-PEO copolymer was almost eight times lower than that on the control PDLLA surface. On the surfaces containing 5% and 20% GRGDSG-PEO-b-PDLLA copolymer, the number of cells increased 6- and 3-fold respectively, compared to the PDLLA-b-PEO copolymer alone. On PDLLA-b-PEO copolymer alone, the cells were typically round and non-spread, whereas on GRGDSG-modified surfaces the cell spreading areas approached those found on PDLLA, reaching values of 991 microm(2) and 611 microm(2) for 5% and 20% GRGDSG respectively, compared to 958 microm(2) for PDLLA. The cells on GRGDSG-grafted copolymers were able to form vinculin-containing focal adhesion plaques, to synthesize DNA and even proliferate in a serum-free medium, which indicates specific binding to the GRGDSG sequences through their adhesion receptors.
- MeSH
- biokompatibilní potahované materiály aplikace a dávkování chemie MeSH
- buněčná adheze účinky léků MeSH
- buněčné kultury metody MeSH
- financování organizované MeSH
- krysa rodu rattus MeSH
- kultivované buňky MeSH
- myocyty hladké svaloviny cytologie fyziologie účinky léků MeSH
- oligopeptidy aplikace a dávkování chemie MeSH
- polyestery chemie MeSH
- potkani Wistar MeSH
- povrchové vlastnosti MeSH
- proliferace buněk účinky léků MeSH
- svaly hladké cévní cytologie fyziologie účinky léků MeSH
- testování materiálů MeSH
- tkáňové inženýrství metody MeSH
- viabilita buněk účinky léků MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- MeSH
- buněčná adheze fyziologie MeSH
- buněčné dělení fyziologie MeSH
- buněčný cyklus fyziologie MeSH
- cévní endotel fyziologie MeSH
- finanční podpora výzkumu jako téma MeSH
- oligopeptidy fyziologie MeSH
- polymery farmakologie chemická syntéza MeSH
- svaly hladké cévní fyziologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- MeSH
- finanční podpora výzkumu jako téma MeSH
- gelová chromatografie MeSH
- krysa rodu rattus MeSH
- laktáty farmakokinetika MeSH
- magnetická rezonanční spektroskopie MeSH
- nosiče léků farmakokinetika MeSH
- polyethylenglykoly farmakokinetika MeSH
- polymery farmakokinetika MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
