UVA photons are less energetic than UVB photons but they are more abundant in solar radiation. Modern tools have shown that UVA light has serious adverse effects on the skin. We investigated the effect of consuming Lonicera caerulea berries on UVA-induced damage in SKH-1 mice. The mice were fed a diet containing L. caerulea berries (10%, w/w) for 14 days before a single UVA (30 J/cm(2)) treatment. Effects on haematological and antioxidant parameters were evaluated 4 and 24h after irradiation. The bioavailability of L. caerulea phenolics was also assessed. Consuming the L. caerulea berry-enriched diet caused reduced malondialdehyde production and increased catalase activity and glutathione levels were found in skin and erythrocytes. UVA-induced NADPH:quinone oxidoreductase-1 and gamma-L-glutamate-L-cysteine ligase protein in skin were reduced in mice fed L. caerulea berries. Enhanced heme oxygenase-1 level in skin, interleukin-17 in plasma and reduced interleukin-12 levels in plasma were found in the mice on the experimental diet. Histological (pyknotic) changes in the nuclei of basal cells induced by UVA exposure were reduced in L. caerulea berry consuming animals. HLPC-MS analysis showed high concentrations of hippuric acid, one of the main metabolites of aromatic amino acids and phenolic compounds, in skin, liver, urine and faeces of mice consuming the berries. Taken together, consumption of L. caerulea berries affords protection from the adverse effects of a single UVA exposure mainly via modulation of antioxidant parameters.
- MeSH
- antioxidancia metabolismus MeSH
- dieta * MeSH
- enzymy metabolismus MeSH
- erytrocyty metabolismus účinky záření MeSH
- glutathion metabolismus MeSH
- hippuráty analýza moč MeSH
- interleukin-12 krev MeSH
- interleukin-17 krev MeSH
- játra chemie MeSH
- kůže metabolismus patologie účinky záření MeSH
- Lonicera chemie metabolismus MeSH
- malondialdehyd metabolismus MeSH
- myši bezsrsté MeSH
- myši MeSH
- ovoce chemie metabolismus MeSH
- ultrafialové záření * MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Solar ultraviolet radiation is a major environmental factor that has serious adverse effects on the structure and function of the skin. Although the UVB waveband (295-315 nm) represents only 5-10% of incoming UV light, it is very damaging to the skin. The aim of this study was to investigate the effect of Lonicera caerulea berries on UVB-induced damage to SKH-1 hairless mice. Mice were fed a L. caerulea berry-enriched diet (10%, w/w) for 14 days before a single UVB (1000 mJ cm(-2)) treatment. Effects on health status, antioxidant enzyme activity and expression, and DNA damage were evaluated. The bioavailability of L. caerulea phenolic components was also assessed. We found that feeding with L. caerulea berries prevented a decrease in catalase activity and stimulated NADPH quinone oxidoreductase-1, heme oxygenase-1, and gamma-glutamylcysteine synthetase catalytic and modulatory subunit expression in UVB exposed mice. Administration of the L. caerulea berry-enriched diet led to an increase in UVB-reduced interleukin-17 levels and a decrease in keratinocyte-derived chemokine protein expression that was enhanced after UVB treatment. Further, L. caerulea berries reduced UVB-induced DNA damage evaluated as number of single strand breaks, cyclobutane-pyrimidine dimer formation and H2AX phosphorylation, a marker of double strand breaks. Taken together, we provide evidence that oral administration of L. caerulea berries to mice affords at least partial protection from the adverse effects of a single UVB exposure via modulation of antioxidant enzyme activity/expression and reduction of DNA damage.
- MeSH
- dieta * MeSH
- erytrocyty metabolismus účinky záření MeSH
- fenoly analýza moč MeSH
- glutamátcysteinligasa metabolismus MeSH
- hemoxygenasa-1 metabolismus MeSH
- histony metabolismus MeSH
- interleukin-17 metabolismus MeSH
- játra enzymologie účinky záření MeSH
- katalasa metabolismus MeSH
- kůže enzymologie patologie účinky záření MeSH
- Lonicera chemie metabolismus MeSH
- myši bezsrsté MeSH
- myši MeSH
- NAD(P)H dehydrogenasa (chinon) metabolismus MeSH
- ovoce chemie metabolismus MeSH
- pilotní projekty MeSH
- poškození DNA účinky záření MeSH
- ultrafialové záření * MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
This study investigated the effect of one-week consumption of 165 g/day fresh blue honeysuckle berries (208 mg/day anthocyanins) in 10 healthy volunteers. At the end of intervention, levels of benzoic (median 1782 vs 4156), protocatechuic (709 vs 2417), vanillic (2779 vs 4753), 3-hydroxycinnamic (143 vs 351), p-coumaric (182 vs 271), isoferulic (805 vs 1570), ferulic (1086 vs 2395), and hippuric (194833 vs 398711 μg/mg creatinine) acids by LC/MS were significantly increased in the urine. Clinical chemistry safety markers were not altered. Oxidative stress markers, erythrocyte glutathione peroxidase (0.73 vs 0.88 U/g Hb) and catalase (2.5 vs 2.8 μkat/g Hb) activities, and erythrocyte/plasma thiobarbituric acid reactive substance (522 vs 612/33 vs 38 μmol/g Hb/protein) levels were significantly increased, without change in plasma antioxidant status. Nonsignificant changes of advanced oxidation protein products and oxidized LDL were observed. The results provide a solid base for further study of metabolite excretion and antioxidant parameters after ingestion of anthocyanins.
- MeSH
- anthokyaniny aplikace a dávkování MeSH
- antioxidancia metabolismus MeSH
- biologické markery moč MeSH
- chromatografie kapalinová MeSH
- cinnamáty moč MeSH
- dospělí MeSH
- erytrocyty metabolismus MeSH
- glutathionperoxidasa krev MeSH
- hippuráty moč MeSH
- hmotnostní spektrometrie MeSH
- hydroxybenzoáty moč MeSH
- katalasa krev MeSH
- kyselina benzoová moč MeSH
- kyselina vanilová moč MeSH
- kyseliny kumarové moč MeSH
- látky reagující s kyselinou thiobarbiturovou metabolismus MeSH
- lidé MeSH
- lipoproteiny LDL krev MeSH
- Lonicera chemie MeSH
- metabolom * MeSH
- ovoce chemie MeSH
- oxidační stres účinky léků MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- klinické zkoušky MeSH
- práce podpořená grantem MeSH
Cranberry (Vaccinium macrocarpon Ait. Ericaceae) fruits and juice are widely used for their antiadherence and antioxidative properties. Little is known however about their effects on clinical chemistry markers after long-term consumption. This study was conducted to evaluate the effect of three commercial cranberry products, NUTRICRAN90S, HI-PAC 4.0, and PACRAN on the antioxidative status of rodents, divided into three experimental groups. The products were given as dietary admixtures (1500 mg of product/kg of stock feed) for 14 weeks to male Wistar rats (Groups 2-4) and a control Group 1 which received only stock feed. There were no significant cranberry treatment-related effects on oxidative stress parameters, catalase, glutathione peroxidase, glutathione reductase, glutathione transferase, superoxide dismutase, total antioxidant capacity, thiobarbituric acid reactive substances, advanced oxidation protein products, total SH-groups, or any other measured clinical chemistry markers. Hematological parameters, body weight, and food consumption were also unaffected by intake of cranberries. Only liver glutathione reductase activity and glutathione levels were significantly lower in Group 4 than in Group 1. Plasma alkaline phosphatase alone was significantly decreased in Group 2. No gross pathology, effects on organ weights, or histopathology were observed. No genotoxicity was found, and total cytochrome P450 level in liver was unaffected in all groups. The levels of hippuric acid and several phenolic acids were significantly increased in plasma and urine in Groups 2-4. The concentration of anthocyanins was under the detection threshold. The dietary addition of cranberry powders for 14 weeks was well tolerated, but it did not improve the antioxidative status in rats.
- MeSH
- antioxidancia aplikace a dávkování farmakologie MeSH
- glutathionperoxidasa metabolismus MeSH
- katalasa metabolismus MeSH
- krysa rodu rattus MeSH
- oxidační stres účinky léků MeSH
- pilotní projekty MeSH
- potkani Wistar MeSH
- rostlinné přípravky aplikace a dávkování farmakologie MeSH
- Vaccinium macrocarpon chemie MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Research Support, N.I.H., Extramural MeSH
Anthocyanins from the fruit Lonicera caerulea L. var. kamtschatica (blueberry honeysuckle, Caprifoliaceae) were studied via (semi)preparative chromatographic fractionation followed by MS and μLC/MS analysis. The extraction procedure was optimized with respect to analytical purposes as well as its potential use for the preparation of nutraceuticals. The highest yield of anthocyanins was obtained using acidified methanol as the extraction medium. A comparable total anthocyanin content was obtained using a mixture of methanol and acetone. However, when Lonicera anthocyanins were in contact with acetone, a condensation reaction occurred to a large extent and related 5-methylpyranoanthocyanins were found. The effect of other extraction media, including ethanol as a "green" solvent, is also discussed. The potential of two fractionation procedures for extract purification differing in their chromatographic selectivity and scale was studied (i.e. using a Sephadex LH-20 gel column and a reversed phase). Fractions obtained by both procedures were used for a detailed analysis. MS and μLC/MS(2) methods were used for monitoring anthocyanin and 5-methylpyranoderivatives content as well as identifying less common and more complex dyes (dimer of cyanidin-3-hexoside, cyanidin-ethyl-catechin-hexosides, etc.). These more complex dyes are most likely formed during fruit treatment.
- MeSH
- anthokyaniny analýza izolace a purifikace MeSH
- chemická frakcionace přístrojové vybavení metody MeSH
- Lonicera chemie MeSH
- rostlinné extrakty analýza izolace a purifikace MeSH
- tandemová hmotnostní spektrometrie metody MeSH
- vysokoúčinná kapalinová chromatografie přístrojové vybavení metody MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- práce podpořená grantem MeSH
Cílem studie bylo ověřit účinnost kombinace glukosamin sulfátu a chondroitin sulfátu (Proenzi Premium, Walmark) samostatně nebo v kombinaci s rehabilitací. Do studie bylo zařazeno 60 pacientů ve věku 36-70 let s potvrzenou gonartrozou II. az II. rtg stadia. Pacienti byli roztříděni do tří skupin. První skupina byla kontrolní, bez medikace, bez rehabilitace, druhá a třetí skupina užívala glukosamin sulfát 1500 mg a chondrotin sulfát 1200mg denně perorálně po dobu tří měsíců bez rehabilitace, resp. s rehabilitací. Na sledování účinku se použil klinický test WOMAC, visuální analogová škála CVAS) bolesti kolenního kloubu, přítomnost otoku, palpační bolestivost mediálního kondylu femoru a rozsah pohybu - flexe. Hodnocení probíhalo ve třech měřeních (vstupní, po 3 měsících, po 6 měsících). Při užívání kombinace glukosaminu a chondrotiťmu se významně redukovaly některé symptomy osteoartrózy. Došlo k významnému snížení pocitu bolesti (jak dle indexu WOMAC, tak VAS) a indexu pohyblivosti WOMAC. Ve skupině s medikací bez rehabilitace došlo navíc ke snížení indexu ztuhlosti. Současná rehabilitace neměla vliv na ztuhlost, měla ale naopak příznivější vliv na rozsah flexe.
- Klíčová slova
- Proenzi Premium,
- MeSH
- artróza kolenních kloubů farmakoterapie MeSH
- bolest farmakoterapie klasifikace MeSH
- chondroitinsulfáty aplikace a dávkování terapeutické užití MeSH
- financování organizované MeSH
- glukosamin aplikace a dávkování terapeutické užití MeSH
- kombinovaná farmakoterapie MeSH
- lidé středního věku MeSH
- lidé MeSH
- měření bolesti statistika a číselné údaje MeSH
- rehabilitace MeSH
- senioři MeSH
- stupeň závažnosti nemoci MeSH
- výsledek terapie MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- hodnotící studie MeSH
V práci jsou shrnuty výsledky podávání laktoferrinu obsaženého v přípravku Acneon tablety (firma Walmark, a. s.) u pacientů trpících acne papulopustulosa I.– IV. st. Byl hodnocen účinek jak na nezánětlivé, tak na zánětlivé morfy akné, snášenlivost přípravku a spokojenost pacientů. Výsledkem dvouměsíčního sledování bylo statisticky významné snížení počtu jak komedonů, tak papulopustul.
In this work we summarize the results of lactoferrin (included in Acneon tablets preparate, Walmark) administration in patients suffering from acne papulopustulosa (grade I–IV). We evaluate an effect on both non-inflammatory and inflammatory acne lesions, on tolerance of a preparation and on satisfaction of patients. The outcome of two month surveillance was statistically significant reduction in number of both comedones and papulopustules.
- MeSH
- acne vulgaris farmakoterapie klasifikace MeSH
- hodnocení léčiv MeSH
- laktoferrin aplikace a dávkování MeSH
- mladý dospělý MeSH
- potravní doplňky využití MeSH
- výsledek terapie MeSH
- Check Tag
- mladý dospělý MeSH
- Publikační typ
- přehledy MeSH
The effect of Lonicera caerulea L. (blue honeysuckle) phenolic fraction (18.5% anthocyanins) on cell viability and against oxidative damage in low density lipoproteins (oxLDL), in rat microsomes and in primary cultures of rat hepatocytes and human umbilical vein endothelial cells (HUVEC), was tested. The phenolic fraction was nontoxic to rat hepatocytes and HUVEC at tested concentrations (1-1000 microg/mL) and time intervals up to 24 h inclusive. Phenolic fraction inhibited rat liver microsome peroxidation, induced by tert-butyl hydroperoxide (tBH), with IC(50) values of 160 +/- 20 microg/mL. The fraction at 0.5, 1.0, and 2.0 microg/mL delayed LDL oxidation, induced by Cu(2+), by 130 +/- 20%, 200 +/- 30%, and 400 +/- 10%, respectively. The treatment of HUVEC with oxidatively modified LDL induced an increase in lactate dehydrogenase (LDH) leakage and thiobarbituric acid reactive substances (TBARS) formation, and resulted in lower formazan formation from 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) uptake, most pronounced for 200 microg/mL (24 h oxidation) after 2 h of incubation. The protective effect of the phenolic fraction against cell damage caused by oxLDL was noted at 0.1 microg/mL for HUVEC and against tBH at 1000 microg/mL for both HUVEC and hepatocytes. The observed protective effects were probably due to the antioxidant properties of L. caerulea constituents, mainly anthocyanins. Microsome peroxidation and LDL oxidation inhibition results provide promising perspectives into the prevention of some oxidative stress-associated diseases. Other data are important in in vitro systems but seem to be accidental in vivo.
- MeSH
- antioxidancia farmakologie MeSH
- Caprifoliaceae chemie MeSH
- cévní endotel metabolismus účinky léků MeSH
- dospělí MeSH
- hepatocyty metabolismus účinky léků MeSH
- krysa rodu rattus MeSH
- kultivované buňky MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- oxidační stres účinky léků MeSH
- potkani Wistar MeSH
- rostlinné extrakty farmakologie MeSH
- těhotenství MeSH
- zvířata MeSH
- Check Tag
- dospělí MeSH
- krysa rodu rattus MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- těhotenství MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- práce podpořená grantem MeSH
