Závěrečná zpráva o řešení grantu Interní grantové agentury MZ ČR
Přeruš. str. : il., tab. ; 30 cm
Svalovou excizi získanou během thymektomie u pacientů s myasthenia gravis budeme analyzovat pomocí moderních enzymově histochemických, imunohistochemických metod a budeme definovat spektrum morfologických změn v kosterní svalovině u tohoto onemocnění. Upřípadů myasthenia gravis s přítomností zánětlivého infiltrátu ve svalové biopsii chceme stanovit znaky, které je umožní odlišit od definovaných zánětlivých myopatií. Na podkladě korelačních studií se budeme snažit stanovit prognostický význam morfologických znaků získaných ze svalové biopsie u pacientů s myasthenia gravis, což může vést k cílenější péči o tyto pacienty.; In our large series of muscles excisions we would like to define by use enzyme histochemistry and immunohistochemistry the spectrum of changes in skeletal muscle in myasthenia gravis, which have not yet been reported in the literature. For the cases where the secondary lymphocytic infiltrate is present in the muscle biopsy we will try to estimate the differential diagnostic criteria for distinguishing between the myasthenia gravis and the primary idiopathic myositides. We will try to estimate the histopathologic prognostic markers obtainable from the MG muscle biopsy during thymectomy which could further enhance management of the individual disease.
- MeSH
- Biopsy MeSH
- Immunohistochemistry methods utilization MeSH
- Myasthenia Gravis diagnosis MeSH
- Prognosis MeSH
- Thymectomy MeSH
- Conspectus
- Patologie. Klinická medicína
- NML Fields
- neurologie
- neurochirurgie
- histologie
- NML Publication type
- závěrečné zprávy o řešení grantu IGA MZ ČR
- MeSH
- Autoimmune Diseases diagnosis genetics MeSH
- Biopsy methods utilization MeSH
- Financing, Organized MeSH
- Humans MeSH
- Lymphocytes metabolism MeSH
- Myasthenia Gravis genetics complications MeSH
- Polymyositis etiology genetics complications MeSH
- Thymoma genetics complications MeSH
- Check Tag
- Humans MeSH
The aim of the study is to provide evidence that the lymphocytic infiltration of myasthenia gravis (MG) muscle do not represent a true autoimmune myositis, rather an infiltration by naive lymphocytes derived from lymphocyte-rich thymomas. Muscle biopsies from 179 patients with pure MG, 6 thymoma patients without MG and 15 patients with definite polymyositis were analyzed. In 18 patients with MG (all associated with lymphocyte-rich thymomas) and in two thymoma patients without MG, lymphocytic infiltrates were identified in muscles. By use of immunohistochemistry, we demonstrated that the lymphocytes in MG differ from those in polymyositis, being mature but in contrast to polymyositis naive CD45RA+ T lymphocytes. We suggest that the lymphocytic infiltrates in patients with MG and thymoma represent an infiltration of muscle by thymoma-derived mature but naive T cells. The finding of CD8+CD45RA+ lymphocytes in muscle may signify an underlying thymoma and should not be misdiagnosed as polymyositis.
- MeSH
- Lymphocyte Activation MeSH
- Leukocyte Common Antigens immunology MeSH
- CD8 Antigens immunology MeSH
- Biomarkers analysis metabolism MeSH
- Biopsy MeSH
- Cell Differentiation MeSH
- Diagnosis, Differential MeSH
- Adult MeSH
- Phenotype MeSH
- Financing, Organized MeSH
- Muscle, Skeletal immunology pathology physiopathology MeSH
- Middle Aged MeSH
- Humans MeSH
- Lymphocytes immunology pathology MeSH
- Myasthenia Gravis immunology physiopathology pathology MeSH
- Polymyositis immunology physiopathology pathology MeSH
- Cell Proliferation MeSH
- Aged MeSH
- Thymoma immunology complications physiopathology MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Aged MeSH
- Female MeSH
