Matrix Gla protein (MGP), a natural inhibitor of calcification, strongly correlates with the extent of coronary calcification. Vitamin K is the essential cofactor for the activation of MGP. The nonphosphorylated-uncarboxylated isoform of MGP (dp-ucMGP) reflects the status of this vitamin. We investigated whether there is an association between dp-ucMGP and stiffness of elastic and muscular-type large arteries in a random sample from the general population. In a cross-sectional design, we analyzed 1087 subjects from the Czech post-MONICA study. Aortic and femoro-popliteal pulse wave velocities (PWVs) were measured using a Sphygmocor device. Dp-ucMGP concentrations were assessed in freshly frozen samples by enzyme-linked immunosorbent assay methods using the InaKtif MGP iSYS pre-commercial kit developed by IDS and VitaK. Aortic PWV significantly (P<0.0001) increased across the dp-ucMGP quartiles. After adjustment for all potential confounders, aortic PWV independently correlated with dp-ucMGP (with beta coefficient (s.d.) 11.61 (5.38) and P-value=0.031). In a categorized manner, subjects in the top quartile of dp-ucMGP (⩾ 671 pmol l(-1)) had a higher risk of elevated aortic PWV, with corresponding adjusted odds ratio (95% confidence interval) 1.73 (1.17-2.5). In contrast, no relation between dp-ucMGP and femoro-popliteal PWV was found. In conclusion, increased dp-ucMGP, which is a circulating biomarker of vitamin K status and vascular calcification, is independently associated with aortic stiffness, but not with stiffness of distal muscular-type arteries.
- MeSH
- analýza pulzové vlny MeSH
- biologické markery krev MeSH
- dospělí MeSH
- ELISA MeSH
- extracelulární matrix - proteiny krev MeSH
- fosforylace MeSH
- lidé středního věku MeSH
- lidé MeSH
- lineární modely MeSH
- logistické modely MeSH
- multivariační analýza MeSH
- nemoci aorty krev diagnóza patofyziologie MeSH
- odds ratio MeSH
- onemocnění periferních arterií krev diagnóza patofyziologie MeSH
- proteiny vázající vápník krev MeSH
- průřezové studie MeSH
- rizikové faktory MeSH
- senioři MeSH
- tuhost cévní stěny * MeSH
- upregulace MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika MeSH
INTRODUCTION: Glioblastoma multiforme (GBM) is the most malignant primary brain tumor in adults. Recent whole-genome studies revealed novel GBM prognostic biomarkers such as mutations in metabolic enzyme IDH-isocitrate dehydrogenases (IDH1 and IDH2). The distinctive mutation IDH1 R132H was uncovered to be a strong prognostic biomarker for glioma patients. We investigated the prognostic role of IDH1 R132H mutation in GBM patients in West Bohemia. METHODS: The IDH1 R132H mutation was assessed by the RT-PCR in the tumor samples from 45 GBM patients treated in the Faculty Hospital in Pilsen and was correlated with the progression free and overall survival. RESULTS: The IDH1 R132H mutation was identified in 20 from 44 GBM tumor samples (45.4%). The majority of mutated tumors were secondary GBMs (16 in 18, 89.9%). Low frequency of IDH1 mutations was observed in primary GBMs (4 in 26, 15.3%). Patients with IDH R132H mutation had longer PFS, 136 versus 51 days (P < 0.021, Wilcoxon), and OS, 270 versus 130 days (P < 0.024, Wilcoxon test). SUMMARY: The prognostic value of IDH1 R132H mutation in GBM patients was verified. Patients with mutation had significantly longer PFS and OS than patients with wild-type IDH1 and suffered more likely from secondary GBMs.
- MeSH
- dospělí MeSH
- glioblastom diagnóza epidemiologie genetika mortalita MeSH
- isocitrátdehydrogenasa genetika MeSH
- Kaplanův-Meierův odhad MeSH
- lidé středního věku MeSH
- lidé MeSH
- nádorové biomarkery genetika MeSH
- nádory mozku diagnóza epidemiologie genetika mortalita MeSH
- prognóza MeSH
- retrospektivní studie MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
OBJECTIVES: Classical and proliferative tumour markers and matrix metalloproteinases and their tissue inhibitors reflect the features of malignancy and are useful in prediction of prognosis in patients with colorectal liver metastases. There is very limited information about their physiological functions during regeneration and healing of liver parenchyma after any type of liver surgery for malignancy. METHODS: The presented study included the patients, who underwent following surgical procedures for CLM, benign liver lesions and inguinal hernias: Group A: 22 patients with inguinal hernias, Group B: 26 patients with benign liver lesions, Group C: 30 patients with colorectal liver metastases (CLM) who were treated by radiofrequency ablation, Group D: 41 patients with CLM who underwent a radical surgical therapy - resection, and Group E: 22 patients with inoperable CLM who underwent an explorative laparotomy without any surgical procedure. RESULTS: The preoperative and postoperative serum levels of CEA, CA 19-9, TK, TPA, TPS, MMP-2, MMP-9, TIMP-1, and TIMP-2 were statistically analyzed and compared within the groups to estimate the influence of a surgical procedure type. These results reflect the influence of surgical procedure on the serum levels of studied tumour markers during operation. CONCLUSIONS: It was the first description using these types of comparison to all metalloproteinases, their inhibitors, and proliferative and classical tumour markers. It could help us to estimate the critical relations of these tumour markers in prognoses of disease free survival or overall survival in patients after a surgical procedure for CLM (Tab. 5, Ref. 26).
- MeSH
- dospělí MeSH
- kolorektální nádory patologie MeSH
- lidé středního věku MeSH
- lidé MeSH
- matrixové metaloproteinasy krev MeSH
- mladiství MeSH
- mladý dospělý MeSH
- nádorové biomarkery krev MeSH
- nádory jater diagnóza sekundární chirurgie MeSH
- prognóza MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- tkáňové inhibitory metaloproteinas krev MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
AIM: The early identification of adverse interactions during mechanical ventilation, investigated by multiplexed immunoanalysis. MATERIALS AND METHODS: Twenty piglets (average age 7 weeks, weight 23 kg) were intubated and divided into groups: A, spontaneously breathing; B, protectively ventilated; C, ventilated with injurious strategy; D, ventilated with lung disability. At the 1st hour (time-1) and 12th hour (time-2) of the study, brain natriuretic peptide (BNP), intercellular cell adhesion molecules (ICAM-1), vascular cell adhesion molecules (VCAM-1), tumor necrosis factor-alpha (TNF-alpha), and interleukin-6 (Il-6) were analyzed in the blood. RESULTS: The injurious ventilated group C exhibited an increase in both cell adhesion molecules (p<0.01), TNF-alpha and BNP (p<0.05) at time-1, and at time-2 further increases (p<0.05). In group D, an increase in ICAM-1 and BNP (p<0.05) at time-1, and increases in Il-6 and ICAM-1 (p<0.05) at time-2, with notable decreases in urine output were observed. Overall, the lung damage correlated with TNF-alpha (r=0.904), Il-6 (r=0.740), and ICAM-1 (r=0.756) levels. CONCLUSION: All five monitored molecules quickly and reliably signaled adverse interactions.
- MeSH
- biologické markery krev MeSH
- časové faktory MeSH
- cévní buněčněadhezivní molekula-1 krev MeSH
- imunoanalýza metody MeSH
- interleukin-6 krev MeSH
- mezibuněčná adhezivní molekula-1 krev MeSH
- modely nemocí na zvířatech MeSH
- multiorgánové selhání krev diagnóza etiologie MeSH
- natriuretický peptid typu B krev MeSH
- plíce patologie patofyziologie ultrastruktura MeSH
- poškození plic krev diagnóza etiologie MeSH
- prasata MeSH
- senzitivita a specificita MeSH
- TNF-alfa krev MeSH
- transmisní elektronová mikroskopie MeSH
- umělé dýchání škodlivé účinky MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Publikační typ
- abstrakt z konference MeSH
- Publikační typ
- abstrakt z konference MeSH
BACKGROUND: MicroRNAs (miRNAs), which are endogenously expressed regulatory noncoding RNAs, have an altered expression in tumor tissues. MiRNAs regulate cancer-related processes such as cell growth and tissue differentiation, and therefore, might function as oncogenes or tumor-suppressor genes. The aim of our study was to assess the expression of mir-20a, let-7a, miR-15a and miR-16 in prostate cancer (PCa) and benign prostatic hyperplasia (BPH) tissue and to investigate the relation between the expression of miRNAs and the clinicopathological features of PCa. PATIENTS AND METHODS: The study group comprised 138 patients: 85 patients with BPH and 53 patients with PCa. The total RNA was isolated from the tissue specimen core and miRNA expressions were quantified using a real-time RT-PCR method (TaqMan MicroRNA Assays). U6snRNA was used for the normalization of the miRNA expression. RESULTS: miR-20a expression was significantly higher in the group of patients with a Gleason score of 7-10 in comparison with the group of patients with a Gleason score of 0-6 (p=0.0082). We found no statistical differences in the miRNA expressions (mir-20a, let-7a, miR-15a and miR-16) in the PCa tissue samples in comparison with the BPH tissue samples. CONCLUSION: Our result shows that the more dedifferentiated PCa cells have a higher expression of miR-20a and this supports the oncogenic role of miR-20a in PCa carcinogenesis. The evaluation of miRNA expression could yield new information about PCa pathogenesis.
- MeSH
- hyperplazie prostaty genetika MeSH
- lidé středního věku MeSH
- lidé MeSH
- mikro RNA genetika MeSH
- nádorové biomarkery analýza genetika MeSH
- nádory prostaty genetika MeSH
- polymerázová řetězová reakce s reverzní transkripcí MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Cíl studie: Autoři podávají přehled o možnosti využití nádorových markerů u epitelových nádorůovarií se zaměřením na jejich použití v rutinní klinické praxi.Typ studie: Souhrnný článek.Název a sídlo pracoviště: Onkologické a radioterapeutické oddělení, Fakultní nemocnice, Plzeň.Předmět a metoda studie: Souhrn publikovaných dat z české a zahraniční odborné literatury.Závěr: Nádorovým markerem první volby je u epitelových nádorů ovarií CA 125. Pro nízkou senzitivitunelze nádorových markerů využít pro screening a primární diagnostiku. Z hlediska prognózyjsou za nepříznivé považovány vysoké předoperační hladiny CA 125. Zároveň se ukazuje,že monitorace hladin CA 125 v průběhu chemoterapie se jeví jako jeden z nejvýznamnějších indikátorůúčinku chemoterapie, a tím i prognózy. Pravidelná monitorace CA 125 v průběhu followup může přispět k časné detekci recidivy nádorového onemocnění. Optimální časový interval prosledování je 3 měsíce po dobu prvních dvou let, dále pak v 6měsíčních intervalech. Z ostatníchnádorových markerů se v rutinní klinické praxi uplatňují zejména CA 19-9 a CA 72-4, a to hlavněu mucinózních epitelových nádorů. Tyto markery ale mají pouze doplňující význam.
Aim of Study: The authors present the view of possible usage of tumour markers in epithelialovarian cancer, and they aim at their use in routine clinic practice.Type of Study: Review article.Setting: Oncology and Radio-Therapeutic Department, Faculty Hospital, Pilsen.Subject and Methods of the Study: Summary of data published in Czech and foreign professionalliterature.Conclusion: In case of epithelium tumours of ovaries, CA 125 is the tumour marker of the fi rst choice.For the reason of low sensitivity, it is not possible to use the tumor markers for screening andprimary diagnostics. Regarding prognoses, high pre-operational levels of CA 125 are consideredunfavourable. At the same time the study demonstrates that CA 125 levels monitoring in the courseof chemotherapy appears to be one of the most signifi cant indicators of chemotherapy effects and,therefore, also of prognoses. Regular CA 125 monitoring in the course of follow up can contributeto early detection of tumour disease relapses. The optimal time interval for monitoring seems to bethree months for the period of the fi rst two years, and then six-month interval. Other tumour markersused in routine clinic practice are mainly CA 19-9 and CA 72-4, particularly in case of mucinoidepithelium tumours. However, these markers are of subsidiary importance only.
