- Publikační typ
- abstrakt z konference MeSH
Secretoneurin (SN) is a 33 amino-acid evolutionary conserved neuropeptide from the chromogranin peptide family. SN's main effects may be cardioprotective and are believed to be mediated through its inhibition of calmodulin-dependent kinase II (CaMKII), which influences intracellular calcium handling. SN inhibition of CaMKII suppresses calcium leakage from the sarcoplasmic reticulum through the ryanodine receptor. This action may reduce the risk of ventricular arrhythmias and calcium-dependent remodelling in heart failure. SN is also involved in reducing the intracellular reactive oxygen species concentration, modulating the immune response, and regulating the cell cycle, including apoptosis. SN can predict mortality in different disease states, beyond the classical risk factors and markers of myocardial injury. Plasma SN levels are elevated soon after an arrhythmogenic episode. In summary, SN is a novel biomarker with potential in cardiovascular medicine, and probably beyond.
- Publikační typ
- časopisecké články MeSH
- přehledy MeSH
BACKGROUND: Total gentamicin is a sum of five congeners C1, C1a, C2, C2a and minor C2b, which differ from each other in their methylation on the purpurosamine ring. Liquid chromatography with mass detection (LC-MS/MS) and specified calibration material enables the concentration of total gentamicin and its individual congeners to be analysed. METHODS: 50 µL serum was precipitated with acetonitrile in the presence of 0.5 mol/L formic acid. A RP BEH C18 1.7 µm 2.1x50 mm column maintained at 30 °C and tobramicin as the internal standard were used. Mass detection was performed in positive electrospray. The gentamicin results were compared with fluorescence polarization immunoassay (FPIA) and chemiluminiscent microparticle immunoassay (CMIA). Passing-Bablock regression analysis and Bland-Altman analysis were used. RESULTS: Calibration curves for individual gentamicin congeners were linear with correlation coefficients between 0.997 and 0.998. Recovery was 91.6-102.0% and the coefficients of variation 1.4-8.4%. The total gentamicin concentration was compared with immunoassay FPIA (LC-MSgen = 0.9798xPFIAgen) and CMIA (LC MSgen = 0.9835xCMIAgen) both with significant correlation (p < 0.001). CONCLUSION: The LC-MS/MS method is fast and precise and can be applied to routine TDM in patients. Comparing it to immunoassays makes it possible to measure concentration of gentamicin congeners, which may be important in the case of their different pharmacokinetics.
AIMS: The study aims were to verify the serum (S) and synovial fluid (SF) reference intervals (RIs) for human neutrophil defensins (HNP1-3); measure S and SF defensin concentrations in different types of SF, including non-inflammatory, inflammatory non-pyogenic, inflammatory pyogenic, and hemorrhagic; and to compare the HNP1-3 concentrations in SF and S with those of other inflammatory biomarkers. METHODS: SF and S samples were collected from 92 patients. HNP1-3 concentrations were determined using enzyme-linked immunosorbent assays; glucose, lactate, interleukin-6, and procalcitonin using an automatic analyzer; and presepsin using a Pathfast system. There were 61 non-inflammatory, 11 inflammatory non-pyogenic, 11 inflammatory pyogenic, and 9 hemorrhagic SF. Non-inflammatory SF was divided into non-inflammatory normal and non-inflammatory osteoarthritis. The former was used to estimate the HNP1-3 RI in SF and S. RESULTS: The estimated HNP1-3 RIs of SF and S were 12.47-437.42 mg/L and 5.45-44.75 μg/L, respectively. HNP1-3 differed significantly between S and SF and individual groups of SF (P<0.001 and P=0.001, respectively). There were significant relationships between SF HNP1-3 and S HNP1-3 (P<0.001), S C-reactive protein (P<0.001), and S interleukin-6 (P=0.007), and between SF HNP1-3 and SF C-reactive protein (P=0.004) and SF interleukin-6 (P<0.001). The highest kappa coefficient was between SF HNP1-3 and SF interleukin-6 (κ=0.507). CONCLUSIONS: We validated the SF HNP1-3 diagnostic kit and demonstrated that SF and S HNP1-3 are promising biomarkers for distinguishing inflammatory from non-inflammatory joint diseases.
- MeSH
- alfa-defensiny * MeSH
- antigeny CD14 MeSH
- biologické markery MeSH
- C-reaktivní protein MeSH
- ELISA * MeSH
- interleukin-6 MeSH
- lidé MeSH
- peptidové fragmenty MeSH
- synoviální tekutina MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Klíčová slova
- neurodegenerativní markery,
- MeSH
- amyloidní beta-protein mozkomíšní mok MeSH
- biologické markery mozkomíšní mok MeSH
- chemické techniky analytické * metody přístrojové vybavení statistika a číselné údaje MeSH
- dospělí MeSH
- imunoanalýza metody přístrojové vybavení statistika a číselné údaje MeSH
- lidé středního věku MeSH
- lidé MeSH
- neurodegenerativní nemoci * diagnóza klasifikace MeSH
- proteiny tau klasifikace mozkomíšní mok MeSH
- regresní analýza MeSH
- senioři MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- srovnávací studie MeSH
BACKGROUND: Intrathecal IgM synthesis demonstrated either as cerebrospinal fluid (CSF)-restricted oligoclonal (o-) IgM bands or calculated using various formulas has been linked to more aggressive multiple sclerosis (MS) course. However, the proportion of MS patients showing intrathecal IgM synthesis varies largely between studies. We aimed to explore the relation between different formulas and results of o-IgM, and to assess the frequency of o-IgM bands in an unselected series of samples. METHODS: 432 samples were analyzed for o-IgM, o-IgG and quantitative measures of IgM and IgG synthesis. IgM index and formulas of Reiber, Auer and Öhman were compared to the result of the o-IgM test. RESULTS: At the cut-off commonly used, the non-linear formulas for intrathecal synthesis were specific (>94%) but rather insensitive (<40% even at a cut-off of 4 CSF-restricted bands) compared to o-IgM. No significant difference was noted in the performance of different formulas. At a cut-off of 4 bands, 61% of MS patients, but none of the controls were positive for o-IgM. CONCLUSIONS: Formulas for intrathecal IgM synthesis are insensitive compared to o-IgM. We propose to evaluate samples with 2 or 3 extra-CSF IgM bands as borderline and only samples with 4 or more as definitely positive.
- MeSH
- imunoglobulin M MeSH
- lidé MeSH
- oligoklonální proužky * MeSH
- roztroušená skleróza * diagnóza MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
Úvod a cíl studie: Pacienti s chronickým onemocněním ledvin (CKD) často vykazují zvýšenou koncentraci volných lehkých řetězců (FLC) v séru, provázenou změnami v zastoupení obou jejich forem, tj. kappa i lambda řetězců, a v jejich důsledku pak změnou jejich vzájemného poměru (FLC ratio). Cílem této studie bylo ověření referenčních mezí tohoto poměru u pacientů s chronickým selháním ledvin, přičemž ke stanovení koncentrací FLC kappa a FLC lambda byly použity diagnostické prostředky firmy Binding Site. Typ studie: Metodická studie. Název a sídlo pracoviště: Ústav laboratorní diagnostiky, Oddělení klinické biochemie, Fakultní nemocnice Ostrava. Materiál a metody: Do studie bylo zařazeno 150 pacientů s CKD a 17 kontrol. U všech byla stanovena koncentrace kreatininu, FLC kappa, FLC lambda v séru a z výsledků měření pak byly vypočteny odhad glomerulární filtrace dle CKD EPI (GF) a FLC ratio. Podle vypočtené hodnoty GF byli pacienti s CKD, v souladu s klasifikací KDIGO, rozdělení do skupin G2 až G5. K vyloučení monoklonální povahy FLC byla použita standardní elektroforéza sérových proteinů, imunofixační elektroforéza a izoelektrická fokusace v agarózovém gelu s následným afinitním imunoblottingem s protilátkami proti volným lehkým řetězcům kappa a lambda (IEF/AIB). Ke statistickému zpracování dat byly použity software Excel, MedCalc a Project R. Výsledky: S rostoucím stupněm CKD byly pozorovány statisticky významné rozdíly mezi koncentracemi FLC kappa, FLC lambda i FLC ratio v jednotlivých skupinách (P ≤ 0,0001). Tyto parametry také statisticky významně korelovaly s koncentrací kreatininu i s odhadem glomerulární filtrace dle CKD EPI (P < 0,0001). V souladu s pokynem CLSI EP28-A3C byly pro poměr FLC u pacientů s CKD určeny tyto referenční meze: dolní mez 1,01 a horní mez 3,65. Závěr: Praktická aplikace referenčních mezí pro FLC ratio může zvýšit specificitu FLC testu pro detekci monoklonální produkce FLC u pacientů s CKD. Odhad mezí v této studii byl proveden s využitím diagnostických prostředků firmy Binding Site a nemusí být shodný v případě použití jiných diagnostik.
Objective: Patients with chronic kidney disease (CKD) often show an increased serum free light chains concentration (FLC) accompanied by changes in the representation of both forms, i.e., kappa and lambda chains, and consequently by changing their ratio (FLC ratio). The aim of this study was to verify the reference limits of this ratio in patients with chronic renal failure, determined by commercial IVD of Binding Site. Design: Methodological study. Settings: Institute of Laboratory Diagnostics, Department of Clinical Biochemistry, University Hospital Ostrava. Material and methods: The study included 150 patients with CKD and 17 controls. The concentration of creatinine, FLC kappa, FLC lambda was determined, the estimate of glomerular filtration according to CKD-EPI and serum FLC ratio were calculated according to the KDIGO classification, divided into groups G2 to G5. To exclude the presence of monoclonal FLC, serum protein electrophoresis, immunofixation electrophoresis and isoelectric focusing in agarose gel followed by affinity immunoblotting with antibodies against free kappa and lambda light chains (IEF / AIB) were used. Excel, MedCalc and Project R software were used for statistical data processing. Results: Statistically significant differences were observed between concentrations of FLC kappa, FLC lambda and FLC ratio in individual groups (P < 0.0001). These parameters also statistically significantly correlated with creatinine concentration and with the estimate of glomerular filtration according to CKD EPI (P < 0.0001). In accordance with CLSI guideline EP28-A3C, the following reference limits were established for the FLC ratio in patients with CKD: a lower limit of 1.01 and an upper limit of 3.65. Conclusion: Practical application of reference limits for the FLC ratio may increase the specificity of the FLC test for the detection of monoclonal FLC production in patients with CKD. The limits in this study were estimated using Binding Site diagnostics and may not be the same for other diagnostics.
- Klíčová slova
- enzymatické stanovení,
- MeSH
- chronická renální insuficience * diagnóza patofyziologie MeSH
- hodnoty glomerulární filtrace MeSH
- imunoturbidimetrie MeSH
- klinická studie jako téma MeSH
- kreatinin analýza MeSH
- lehké řetězce imunoglobulinů * analýza MeSH
- lidé MeSH
- referenční hodnoty MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- práce podpořená grantem MeSH
- MeSH
- alkoholismus diagnóza krev MeSH
- elektroforéza kapilární metody přístrojové vybavení MeSH
- imunoturbidimetrie metody přístrojové vybavení MeSH
- lidé MeSH
- prediktivní hodnota testů MeSH
- senzitivita a specificita MeSH
- transferin * analogy a deriváty analýza MeSH
- vysokoúčinná kapalinová chromatografie metody přístrojové vybavení MeSH
- Check Tag
- lidé MeSH
IgG kappa and IgG lambda concentrations were quantified in 96 paired CSF and sera using Hevylite™ antibodies in an in-house developed sandwich ELISA method. In 56 of these samples, the results were compared with a qualitative isoelectric focusing/affinity-mediated immunoblotting assay for oligoclonal IgG kappa and IgG lambda. Normal IgG kappa/lambda ratio in the CSF was the same as in serum. In 19/33 patients with intrathecal oligoclonal IgG synthesis, skewed IgG kappa/lambda ratio was observed (increased in 16 and decreased in 3 cases). The analysis of light chain composition of intrathecally synthesised immunoglobulins could contribute to our understanding of intrathecal humoral immune response, although its diagnostic utility is limited.
- MeSH
- ELISA metody MeSH
- imunoglobulin G imunologie MeSH
- imunoglobuliny - kappa-řetězce imunologie MeSH
- imunoglobuliny - lambda-řetězce imunologie MeSH
- lidé MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
