Závěrečná zpráva o řešení grantu Interní grantové agentury MZ ČR
Přeruš. str. : il., tab. ; 30 cm
Aplikace metod klinické neurologie, neuropsychologie, klinické neurofyziologie, MR zobrazení a molekulární biologie zlepší diagnostiku komplikací neurofibromatosy 1. typu s variabilními klinickými, elektrofyziologickými a morfologickými projevy. Stanovení protilátek proti vimentinu a neurofibrominu umožní: a) prognostiku zaměřenou na stanovení biologické aktivity rozvoje komplikací; b) vyčlenit podskupinu pacientů NF1 s vysokým rizikem tumorigenese či přechodu benigního procesu do malignity.; The application of methods clinical neurology, neuropsychology, clinical neurophysiology, MR imaging, and molecular biology will improve diagnostics of complications of neurofibromatosis of type 1 with variable clinical findings. Anti-vimentin and anti-neurotubulin antibodies testing improves assessment of the biological activity of complications of NF1 and helps to select a subgroup of NF1 patients with the high risk of tumorigenesis or malignacy.
- MeSH
- Magnetic Resonance Imaging MeSH
- Molecular Biology methods MeSH
- Neurofibromatosis 1 diagnosis complications MeSH
- Neurophysiology methods MeSH
- Neovascularization, Pathologic MeSH
- Tubulin MeSH
- Vimentin analysis antagonists & inhibitors MeSH
- Conspectus
- Biochemie. Molekulární biologie. Biofyzika
- NML Fields
- neurologie
- biologie
- neurovědy
- NML Publication type
- závěrečné zprávy o řešení grantu IGA MZ ČR
We describe the expression and distribution patterns of nestin, desmin and vimentin in intact and regenerating muscle spindles of the rat hind limb skeletal muscles. Regeneration was induced by intramuscular isotransplantation of extensor digitorum longus (EDL) or soleus muscles from 15-day-old rats into the EDL muscle of adult female inbred Lewis rats. The host muscles with grafts were excised after 7-, 16-, 21- and 29-day survival and immunohistochemically stained. Nestin expression in intact spindles in host muscles was restricted to Schwann cells of sensory and motor nerves. In transplanted muscles, however, nestin expression was also found in regenerating "spindle fibers", 7 and 16 days after grafting. From the 21st day onwards, the regenerated spindle fibers were devoid of nestin immunoreactivity. Desmin was detected in spindle fibers at all developmental stages in regenerating as well as in intact spindles. Vimentin was expressed in cells of the outer and inner capsules of all muscle spindles and in newly formed myoblasts and myotubes of regenerating spindles 7 days after grafting. Our results show that the expression pattern of these intermediate filaments in regenerating spindle fibers corresponds to that found in regenerating extrafusal fibers, which supports our earlier suggestion that they resemble small-diameter extrafusal fibers.
- MeSH
- Desmin analysis MeSH
- Financing, Organized MeSH
- Muscle Fibers, Skeletal chemistry MeSH
- Muscle, Skeletal chemistry transplantation ultrastructure MeSH
- Rats MeSH
- Myoblasts chemistry MeSH
- Muscle Spindles chemistry MeSH
- Rats, Inbred Lew MeSH
- Intermediate Filament Proteins analysis MeSH
- Nerve Tissue Proteins analysis MeSH
- Regeneration MeSH
- Schwann Cells chemistry MeSH
- Vimentin analysis MeSH
- Hindlimb MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Female MeSH
- Animals MeSH
We describe 12 cases of leiomyoma with intracytoplasmic inclusion bodies, which were detected in a group of 447 leiomyomas examined at our institution between December 2005 and March 2006. Ten of these tumors were typical leiomyomas, and two cases represented atypical (bizarre) leiomyoma. In some cases, the presence of intracytoplasmic inclusion bodies resulted in a rhabdoid or skeletal muscle-like appearance of the tumor cells. Ultrastructurally, there were two types of inclusions. One of them consisted of an abnormal aggregation of intermediate and actin filaments. Another type of inclusions was composed of dense granular material without an apparent fibrillar structure. The ultrastructure of the inclusions correlates with immunohistochemical and histochemical stainings. The inclusions with apparent fibrillar arrangements were PAS negative, stained red by trichrome, and were, at least at the periphery, actin-, desmin-, and h-caldesmon-positive. The dense granular inclusions were at least focally PAS-positive, stained red by trichrome, and were negative immunohistochemically. The intracytoplasmic inclusions were found in atypical (bizarre) leiomyomas of the uterus and occasionally in epithelioid leiomyomas and leiomyosarcomas. However, to the best of our knowledge, these inclusions have not been found in typical uterine leiomyomas to date.
- MeSH
- Actins analysis MeSH
- Azo Compounds MeSH
- Biomarkers analysis MeSH
- Inclusion Bodies chemistry ultrastructure MeSH
- Desmin analysis MeSH
- Diagnosis, Differential MeSH
- Adult MeSH
- Eosine Yellowish-(YS) MeSH
- Financing, Organized MeSH
- Immunohistochemistry methods MeSH
- Keratins analysis MeSH
- Leiomyoma diagnosis chemistry ultrastructure MeSH
- Middle Aged MeSH
- Humans MeSH
- Methyl Green MeSH
- MyoD Protein analysis MeSH
- Myogenin analysis MeSH
- Uterine Neoplasms diagnosis chemistry ultrastructure MeSH
- Calmodulin-Binding Proteins analysis MeSH
- Rhabdoid Tumor pathology MeSH
- Periodic Acid-Schiff Reaction MeSH
- Aged MeSH
- Vimentin analysis MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Aged MeSH
- Female MeSH
BACKGROUND: Nestin is a protein belonging to class VI of intermediate filaments that is produced in stem/progenitor cells in the mammalian CNS during development and is consecutively replaced by other intermediate filament proteins (neurofilaments, GFAP). Down-regulated nestin may be re-expressed in the adult organism under certain pathological conditions (brain injury, ischemia, inflammation, neoplastic transformation). Our work focused on a detailed study of the nestin cytoskeleton in cell lines derived from glioblastoma multiforme, because re-expression of nestin together with down-regulation of GFAP has been previously reported in this type of brain tumor. METHODS: Two cell lines were derived from the tumor tissue of patients treated for glioblastoma multiforme. Nestin and other cytoskeletal proteins were visualized using imunocytochemical methods: indirect immunofluorescence and immunogold-labelling. RESULTS: Using epifluorescence and confocal microscopy, we described the morphology of nestin-positive intermediate filaments in glioblastoma cells of both primary cultures and the derived cell lines, as well as the reorganization of nestin during mitosis. Our most important result came through transmission electron microscopy and provided clear evidence that nestin is present in the cell nucleus. CONCLUSION: Detailed information concerning the pattern of the nestin cytoskeleton in glioblastoma cell lines and especially the demonstration of nestin in the nucleus represent an important background for further studies of nestin re-expression in relationship to tumor malignancy and invasive potential.
- MeSH
- Cytoskeleton chemistry ultrastructure MeSH
- Financing, Organized MeSH
- Fluorescent Antibody Technique, Indirect MeSH
- Glioblastoma genetics chemistry pathology ultrastructure MeSH
- Glial Fibrillary Acidic Protein analysis MeSH
- Humans MeSH
- Cell Line, Tumor MeSH
- Intermediate Filament Proteins analysis MeSH
- Nerve Tissue Proteins analysis MeSH
- Vimentin analysis MeSH
- Check Tag
- Humans MeSH
We present, in a 47-year-old man, the first case of the signet-ring stromal tumor of the testis. The tumor was located beneath the tunica albuginea surrounded by the testicular tubules and rete testis. It was sharply circumscribed by a thin and irregular fibrous capsule. Histologically, it was composed of cells with a widespread signet-ring cell change separated by fibrous stroma. In some places, the signet-ring cells formed vague Indian files, thus resembling metastatic carcinomas with signet-ring cell morphology. Under high magnification, most of the cytoplasm of the tumor cells was seen to be replaced by an empty clear vacuole which pushed the nuclei to the periphery of the cells. Some of the nuclei were indented by the cytoplasmic vacuoles, others were without indentation. Only in a small area did the tumor show cells without a signet-ring cell change. They looked like epithelioid fibroblasts forming abortive and vaguely tubular structures. Mitoses and necroses were absent. Mucicarmine and PAS stains were negative. Immunohistochemically, the tumor was vimentin positive and it was negative with antibodies to cytokeratins, inhibin, prostatic acid phosphatase, prostate-specific antigen, smooth muscle actin, S-100 protein, EMA and calretinin. The signet-ring stromal tumor of the testis is thus similar morphologically and immunohistochemically to the signet-ring stromal tumor of the ovary. The patient was free of recurrence and metastasis 3 years after the excision.
- MeSH
- Stromal Cells chemistry pathology MeSH
- Immunoenzyme Techniques MeSH
- Carcinoma, Signet Ring Cell chemistry surgery pathology MeSH
- Middle Aged MeSH
- Humans MeSH
- Biomarkers, Tumor analysis MeSH
- Disease-Free Survival MeSH
- Testicular Neoplasms chemistry surgery pathology MeSH
- Vimentin analysis MeSH
- Treatment Outcome MeSH
- Check Tag
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Publication type
- Case Reports MeSH
