Závěrečná zpráva o řešení grantu Interní grantové agentury MZ ČR
Přeruš. str. : il., tab. ; 30 cm
Aplikace metod klinické neurologie, neuropsychologie, klinické neurofyziologie, MR zobrazení a molekulární biologie zlepší diagnostiku komplikací neurofibromatosy 1. typu s variabilními klinickými, elektrofyziologickými a morfologickými projevy. Stanovení protilátek proti vimentinu a neurofibrominu umožní: a) prognostiku zaměřenou na stanovení biologické aktivity rozvoje komplikací; b) vyčlenit podskupinu pacientů NF1 s vysokým rizikem tumorigenese či přechodu benigního procesu do malignity.; The application of methods clinical neurology, neuropsychology, clinical neurophysiology, MR imaging, and molecular biology will improve diagnostics of complications of neurofibromatosis of type 1 with variable clinical findings. Anti-vimentin and anti-neurotubulin antibodies testing improves assessment of the biological activity of complications of NF1 and helps to select a subgroup of NF1 patients with the high risk of tumorigenesis or malignacy.
- MeSH
- magnetická rezonanční tomografie MeSH
- molekulární biologie metody MeSH
- neurofibromatóza 1 diagnóza komplikace MeSH
- neurofyziologie metody MeSH
- patologická angiogeneze MeSH
- tubulin MeSH
- vimentin analýza antagonisté a inhibitory MeSH
- Konspekt
- Biochemie. Molekulární biologie. Biofyzika
- NLK Obory
- neurologie
- biologie
- neurovědy
- NLK Publikační typ
- závěrečné zprávy o řešení grantu IGA MZ ČR
We describe the expression and distribution patterns of nestin, desmin and vimentin in intact and regenerating muscle spindles of the rat hind limb skeletal muscles. Regeneration was induced by intramuscular isotransplantation of extensor digitorum longus (EDL) or soleus muscles from 15-day-old rats into the EDL muscle of adult female inbred Lewis rats. The host muscles with grafts were excised after 7-, 16-, 21- and 29-day survival and immunohistochemically stained. Nestin expression in intact spindles in host muscles was restricted to Schwann cells of sensory and motor nerves. In transplanted muscles, however, nestin expression was also found in regenerating "spindle fibers", 7 and 16 days after grafting. From the 21st day onwards, the regenerated spindle fibers were devoid of nestin immunoreactivity. Desmin was detected in spindle fibers at all developmental stages in regenerating as well as in intact spindles. Vimentin was expressed in cells of the outer and inner capsules of all muscle spindles and in newly formed myoblasts and myotubes of regenerating spindles 7 days after grafting. Our results show that the expression pattern of these intermediate filaments in regenerating spindle fibers corresponds to that found in regenerating extrafusal fibers, which supports our earlier suggestion that they resemble small-diameter extrafusal fibers.
- MeSH
- desmin analýza MeSH
- financování organizované MeSH
- kosterní svalová vlákna chemie MeSH
- kosterní svaly chemie transplantace ultrastruktura MeSH
- krysa rodu rattus MeSH
- myoblasty chemie MeSH
- nervosvalová vřeténka chemie MeSH
- potkani inbrední LEW MeSH
- proteiny intermediálních filament analýza MeSH
- proteiny nervové tkáně analýza MeSH
- regenerace MeSH
- Schwannovy buňky chemie MeSH
- vimentin analýza MeSH
- zadní končetina MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- ženské pohlaví MeSH
- zvířata MeSH
We describe 12 cases of leiomyoma with intracytoplasmic inclusion bodies, which were detected in a group of 447 leiomyomas examined at our institution between December 2005 and March 2006. Ten of these tumors were typical leiomyomas, and two cases represented atypical (bizarre) leiomyoma. In some cases, the presence of intracytoplasmic inclusion bodies resulted in a rhabdoid or skeletal muscle-like appearance of the tumor cells. Ultrastructurally, there were two types of inclusions. One of them consisted of an abnormal aggregation of intermediate and actin filaments. Another type of inclusions was composed of dense granular material without an apparent fibrillar structure. The ultrastructure of the inclusions correlates with immunohistochemical and histochemical stainings. The inclusions with apparent fibrillar arrangements were PAS negative, stained red by trichrome, and were, at least at the periphery, actin-, desmin-, and h-caldesmon-positive. The dense granular inclusions were at least focally PAS-positive, stained red by trichrome, and were negative immunohistochemically. The intracytoplasmic inclusions were found in atypical (bizarre) leiomyomas of the uterus and occasionally in epithelioid leiomyomas and leiomyosarcomas. However, to the best of our knowledge, these inclusions have not been found in typical uterine leiomyomas to date.
- MeSH
- aktiny analýza MeSH
- azosloučeniny MeSH
- biologické markery analýza MeSH
- buněčná inkluze chemie ultrastruktura MeSH
- desmin analýza MeSH
- diferenciální diagnóza MeSH
- dospělí MeSH
- eosin MeSH
- financování organizované MeSH
- imunohistochemie metody MeSH
- keratiny analýza MeSH
- leiomyom diagnóza chemie ultrastruktura MeSH
- lidé středního věku MeSH
- lidé MeSH
- methylová zeleň MeSH
- MyoD Protein analýza MeSH
- myogenin analýza MeSH
- nádory dělohy diagnóza chemie ultrastruktura MeSH
- proteiny vázající kalmodulin analýza MeSH
- rhabdoidní nádor patologie MeSH
- Schiffova reakce MeSH
- senioři MeSH
- vimentin analýza MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- senioři MeSH
- ženské pohlaví MeSH
BACKGROUND: Nestin is a protein belonging to class VI of intermediate filaments that is produced in stem/progenitor cells in the mammalian CNS during development and is consecutively replaced by other intermediate filament proteins (neurofilaments, GFAP). Down-regulated nestin may be re-expressed in the adult organism under certain pathological conditions (brain injury, ischemia, inflammation, neoplastic transformation). Our work focused on a detailed study of the nestin cytoskeleton in cell lines derived from glioblastoma multiforme, because re-expression of nestin together with down-regulation of GFAP has been previously reported in this type of brain tumor. METHODS: Two cell lines were derived from the tumor tissue of patients treated for glioblastoma multiforme. Nestin and other cytoskeletal proteins were visualized using imunocytochemical methods: indirect immunofluorescence and immunogold-labelling. RESULTS: Using epifluorescence and confocal microscopy, we described the morphology of nestin-positive intermediate filaments in glioblastoma cells of both primary cultures and the derived cell lines, as well as the reorganization of nestin during mitosis. Our most important result came through transmission electron microscopy and provided clear evidence that nestin is present in the cell nucleus. CONCLUSION: Detailed information concerning the pattern of the nestin cytoskeleton in glioblastoma cell lines and especially the demonstration of nestin in the nucleus represent an important background for further studies of nestin re-expression in relationship to tumor malignancy and invasive potential.
- MeSH
- cytoskelet chemie ultrastruktura MeSH
- financování organizované MeSH
- fluorescenční protilátková technika nepřímá MeSH
- glioblastom genetika chemie patologie ultrastruktura MeSH
- gliový fibrilární kyselý protein analýza MeSH
- lidé MeSH
- nádorové buněčné linie MeSH
- proteiny intermediálních filament analýza MeSH
- proteiny nervové tkáně analýza MeSH
- vimentin analýza MeSH
- Check Tag
- lidé MeSH
We present, in a 47-year-old man, the first case of the signet-ring stromal tumor of the testis. The tumor was located beneath the tunica albuginea surrounded by the testicular tubules and rete testis. It was sharply circumscribed by a thin and irregular fibrous capsule. Histologically, it was composed of cells with a widespread signet-ring cell change separated by fibrous stroma. In some places, the signet-ring cells formed vague Indian files, thus resembling metastatic carcinomas with signet-ring cell morphology. Under high magnification, most of the cytoplasm of the tumor cells was seen to be replaced by an empty clear vacuole which pushed the nuclei to the periphery of the cells. Some of the nuclei were indented by the cytoplasmic vacuoles, others were without indentation. Only in a small area did the tumor show cells without a signet-ring cell change. They looked like epithelioid fibroblasts forming abortive and vaguely tubular structures. Mitoses and necroses were absent. Mucicarmine and PAS stains were negative. Immunohistochemically, the tumor was vimentin positive and it was negative with antibodies to cytokeratins, inhibin, prostatic acid phosphatase, prostate-specific antigen, smooth muscle actin, S-100 protein, EMA and calretinin. The signet-ring stromal tumor of the testis is thus similar morphologically and immunohistochemically to the signet-ring stromal tumor of the ovary. The patient was free of recurrence and metastasis 3 years after the excision.
- MeSH
- buňky stromatu chemie patologie MeSH
- imunoenzymatické techniky MeSH
- karcinom z prstenčitých buněk chemie chirurgie patologie MeSH
- lidé středního věku MeSH
- lidé MeSH
- nádorové biomarkery analýza MeSH
- přežití bez známek nemoci MeSH
- testikulární nádory chemie chirurgie patologie MeSH
- vimentin analýza MeSH
- výsledek terapie MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- Publikační typ
- kazuistiky MeSH
- MeSH
- hemangioblastom chemie patologie ultrastruktura MeSH
- lidé MeSH
- nádory měkkých tkání patologie MeSH
- senioři MeSH
- vimentin analýza MeSH
- Check Tag
- lidé MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- kazuistiky MeSH
