This is the first report describing precipitation of bacteriophage particles with zinc chloride as a method of choice to isolate infectious lytic bacteriophages against Pectobacterium spp. and Dickeya spp. from environmental samples. The isolated bacteriophages are ready to use to study various (ecological) aspects of bacteria-bacteriophage interactions. The method comprises the well-known precipitation of phages from aqueous extracts of the test material by addition of ZnCl2, resuscitation of bacteriophage particles in Ringer's buffer to remove the ZnCl2 excess and a soft agar overlay assay with the host bacterium to isolate infectious individual phage plaques. The method requires neither an enrichment step nor other steps (e. g., PEG precipitation, ultrafiltration, or ultracentrifugation) commonly used in other procedures and results in isolation of active viable bacteriophage particles.
- MeSH
- bakteriofágy izolace a purifikace fyziologie MeSH
- časové faktory MeSH
- chloridy metabolismus MeSH
- Enterobacteriaceae virologie MeSH
- mikrobiologie životního prostředí * MeSH
- Pectobacterium virologie MeSH
- rostliny virologie MeSH
- sloučeniny zinku metabolismus MeSH
- virologie metody MeSH
- Publikační typ
- časopisecké články MeSH
Matrix metalloproteinases (MMPs), also known as matrixins, belong to a group of zinc-dependent proteins, which are thought to play a central role in the breakdown of extracellular matrix. Collagen, elastin, gelatin and casein are major components cleaved by MMPs. The breakdown of these components is essential for many physiological processes such as embryonic development, morphogenesis, reproduction, and tissue resorption and remodelling. MMPs also participate in pathological processes such as arthritis, cancer, cardiovascular and neurological diseases. This review summarizes current knowledge regarding these proteins, their participation in physiological and pathophysiological roles, their involvement in activation and inhibition, and their interactions with other metal-binding proteins including metallothioneins.
- MeSH
- cystein metabolismus MeSH
- extracelulární matrix metabolismus MeSH
- inhibitory matrixových metaloproteinas MeSH
- kardiovaskulární nemoci farmakoterapie enzymologie patofyziologie MeSH
- lidé MeSH
- matrixové metaloproteinasy chemie genetika metabolismus MeSH
- nádory farmakoterapie enzymologie patofyziologie MeSH
- regulace genové exprese enzymů MeSH
- sloučeniny zinku chemie metabolismus farmakologie MeSH
- substrátová specifita MeSH
- tkáňové inhibitory metaloproteinas metabolismus MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- přehledy MeSH
- MeSH
- bilirubin metabolismus MeSH
- cholelitiáza diagnóza etiologie terapie MeSH
- Criglerův-Najjarův syndrom metabolismus terapie MeSH
- experimenty na zvířatech MeSH
- hladovění metabolismus MeSH
- hyperbilirubinemie etiologie metabolismus MeSH
- krysa rodu rattus MeSH
- lidé MeSH
- novorozenecká hyperbilirubinemie etiologie metabolismus MeSH
- primární prevence MeSH
- probiotika metabolismus terapeutické užití MeSH
- sloučeniny zinku metabolismus MeSH
- střeva metabolismus MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- lidé MeSH
- zvířata MeSH
