- Klíčová slova
- TIBOLON,
- MeSH
- Cimicifuga * chemie MeSH
- fytoterapie MeSH
- klimakterium * účinky léků MeSH
- klinické zkoušky jako téma MeSH
- lidé MeSH
- norpregneny aplikace a dávkování terapeutické užití MeSH
- třezalka * chemie MeSH
- Check Tag
- lidé MeSH
- ženské pohlaví MeSH
There is a limited number of studies about the constituents of Hypericum olympicum subsp. olympicum and its genotoxic and cytotoxic potency. We examined the possible antigenotoxic/genotoxic properties of methanolic extract of H. olympicum subsp. olympicum (HOE) on human lymphocytes by employing sister chromatid exchange, micronucleus and comet assay and analyzed its chemical composition by GCxGC-TOF/MS. The anti-growth activity against MCF-7 and MDA-MB-231 cell lines was assessed by using the ATP viability assay. Cell death mode was investigated with fluorescence staining and ELISA assays. The major components of the flower and trunk were determined as eicosane, heptacosane, 2-propen-1-ol, hexahydrofarnesyl acetone and α-muurolene. HOE caused significant DNA damage at selected doses (250-750 µg/ml) while chromosomal damage was observed at higher concentrations (500 and 750 µg/ml). HOE demonstrated anti-growth activity in a dose-dependent manner between 3.13-100 µg/ml. Pyknotic nuclei were observed at 100 µg/ml concentration of HOE in both cell lines. In conclusion, HOE demonstrated cytotoxic effects in a cell type-dependent manner, however its genotoxic effects were observed at relatively higher doses.
- MeSH
- buněčná smrt účinky léků MeSH
- chromatografie MeSH
- fluorescenční spektrometrie MeSH
- inhibitory růstu MeSH
- lidé MeSH
- lymfocyty účinky léků MeSH
- nádorové buněčné linie * účinky léků MeSH
- nádory prsu terapie MeSH
- statistika jako téma MeSH
- třezalka * chemie toxicita MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- práce podpořená grantem MeSH
The worldwide growing interest in traditional medicines, including herbal medicines and herbal dietary supplements, has recently been accompanied by concerns on quality and safety of this type of health care. The content of nutritional and potentially toxic elements in medicinal plants is of paramount interest as it may vary remarkably according to different environmental and ecophysiological factors. In this study, the concentrations of essential and non-essential trace elements-Co, Cr, Cu, Ni, Sr, and Zn-were determined in the roots and aerial parts of the worldwide distributed and economically important medicinal herb Hypericum perforatum L. (St. John's wort) and in its growing substrate. Most of the analyzed trace elements varied considerably in the plant parts according to edaphic conditions and soil geochemistry. However, uptake and retention in H. perforatum compartments of Co, Cr, and Ni, which markedly differentiated the investigated soils, were controlled by excluding mechanisms of the plant. Despite this, the Ni concentrations in the aerial parts, commonly used in herbal preparations, of H. perforatum plants from serpentine soils were not insignificant in relation to eventual human consumption. Good practice to assure the herbal product quality of H. perforatum collected from the wild cannot ignore the thorough understanding of the geolithological and geochemical features of the harvesting areas.
Hypericum japonicum Thunb. ex Murray is traditionally used in Nepal to treat several diseases, among whom inflammation and acute pain. Although several secondary metabolites from the same Hypericum species have been already characterized and considered for their pharmacological use, an exhaustive phytochemical characterization of H. japonicum from Nepal is lacking, as well as the assessment of its potential pharmacological properties. Hence, the aims of this study were the characterization of a methanolic extract of H. japonicum (HJME) collected from the Northern region of Nepal by LC-MSn and UPLC-QTOF. The assessment of in vitro inhibition of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and activator protein 1 (AP-1) transcription factors and HJME's cytotoxic effect on human cell lines was performed to evaluate the potential use of this herb as a source of anti-inflammatory and cytotoxic lead compounds. Fifty-seven phytoconstituents were identified, being mainly flavonoids, phloroglucinols, phenolic acids and xanthones. Although compounds characteristic of H. japonicum were detected (quercetin, quercetin-7-O-α-l-rhamnoside, quercitrin and hyperoside), several others are here reported for the first time in this species. The results from bioassays indicated that HJME could significantly reduce the viability of human THP-1 cells (IC50 = 5.4 ± 1.1 μg mL-1), showing the promising potential of HJME as anti-tumor agent. Furthermore, HJME significantly decreased the activation of both NF-κB and AP-1 at the concentration of 2 μg mL-1. Overall, these data suggest that H. japonicum from Nepal could be used as a source of potential natural anti-inflammatory and anti-tumor lead compounds.
- MeSH
- antiflogistika farmakologie MeSH
- antitumorózní látky farmakologie MeSH
- biotest MeSH
- buněčné linie MeSH
- chromatografie kapalinová MeSH
- flavonoidy farmakologie MeSH
- floroglucinol farmakologie MeSH
- hmotnostní spektrometrie MeSH
- hydroxybenzoáty farmakologie MeSH
- inhibiční koncentrace 50 MeSH
- lidé MeSH
- NF-kappa B - podjednotka p50 antagonisté a inhibitory MeSH
- rostlinné extrakty farmakologie MeSH
- THP-1 buňky MeSH
- transkripční faktor AP-1 antagonisté a inhibitory MeSH
- třezalka chemie MeSH
- viabilita buněk MeSH
- xantony farmakologie MeSH
- zánět MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Nepál MeSH
Hyperforin is a prenylated phloroglucinol derivative which occurs in the plant Hypericum perforatum L. It has several medicinally important properties (antidepressant, anti-inflammatory, proapoptotic, antibacterial and antiangiogenic). To enable its medicinal use, it is necessary to investigate its potential genotoxic effect on human cell lines. We also observed an anticlastogenic effect of hyperforin towards the indirect mutagen benzo(a)pyrene. Benzo(a)pyrene is a widespread polycyclic aromatic hydrocarbon. We performed experiments on human tumor cell line HepG2, using the in vitro mammalian chromosome aberration test. We investigated two cytogenic parameters: the number of aberrant metaphases and the total number of chromosome aberrations. We found out that hyperforin was not genotoxic on human cell line HepG2. The second part of our results implies that hyperforin had an anticlastogenic effect against the indirect mutagen benzo(a)pyrene in our experimental conditions. In future we will continue our research by using another range of hyperforin concentrations, other cell lines and other chemical mutagens.
- MeSH
- antidepresiva terapeutické užití toxicita MeSH
- antiflogistika terapeutické užití toxicita MeSH
- antimutagenní látky terapeutické užití toxicita MeSH
- benzopyreny * chemie MeSH
- biomedicínský výzkum MeSH
- buněčné linie cytologie účinky léků MeSH
- buňky Hep G2 cytologie účinky léků MeSH
- chromozomální aberace * chemicky indukované účinky léků MeSH
- lidé MeSH
- statistika jako téma MeSH
- třezalka * chemie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- práce podpořená grantem MeSH
- přehledy MeSH
Hypericin, the photoactive compound of Hypericum perforatum, is probably the most powerful photosensitizer found in nature. This compound has shown high potency in the photodynamic treatment of tumor cells. However, there is only limited knowledge regarding the photodynamic effect of hypericin on nonmelanoma skin cancer cells. The aim of this prospective study was to investigate the efficacy of photodynamic therapy with topical application of an extract of H. perforatum in actinic keratosis, basal cell carcinoma (BCC) and morbus Bowen (carcinoma in situ). The study was carried out on 34 patients--eight with actinic keratoses (AKs), 21 with BCC and five with Bowen's disease. The extract of H. perforatum was applied on the skin lesions under occlusion and that was followed by irradiation with 75 J cm(-2) of red light 2 h later. The treatment was performed weekly for 6 weeks on average. The percentage of complete clinical response was 50% for AKs, 28% in patients with superficial BCC and 40% in patients with Bowen's disease. There was only a partial remission seen in patients with nodular BCCs. A complete disappearance of tumor cells was found in the histologic preparation of 11% of patients with superficial BCCs and 80% in the patients with Bowen's disease. All patients complained of burning and pain sensations during irradiation. Although the results of this first clinical trial could be regarded as disappointing, there are still possibilities for improvement. Better preparation of the lesions, enhancement of hypericin delivery and other types of light exposure procedures could significantly improve the clinical outcomes of this relatively inexpensive treatment modality.
- MeSH
- antitumorózní látky terapeutické užití MeSH
- aplikace lokální MeSH
- bazocelulární karcinom farmakoterapie MeSH
- Bowenova nemoc farmakoterapie MeSH
- dospělí MeSH
- financování organizované MeSH
- fotochemoterapie metody MeSH
- fytoterapie MeSH
- keratóza farmakoterapie MeSH
- lidé středního věku MeSH
- lidé MeSH
- molekulární struktura MeSH
- nádory kůže farmakoterapie MeSH
- perylen analogy a deriváty terapeutické užití MeSH
- pilotní projekty MeSH
- rostlinné přípravky terapeutické užití MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- třezalka chemie MeSH
- výsledek terapie MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- klinické zkoušky MeSH
Třezalka (Hypericum perforatum L.) je tradičně využívaná léčivá rostlina v alternativní i klasické medicíně. V současnosti jsou k dispozici poznatky o fyziologické aktivitě jednotlivých sloučenin, které třezalka syntetizuje ve svých pletivech. Nejvyšší pozornost je věnována hypericinu a hyperforinu. Tyto organické sloučeniny nacházejí uplatnění v léčbě depresivních stavů, v protinádorové (fotodynamické) terapii a jako antimikrobiální a virostatické agens. V předkládaném souhrnu jsou ve stručnosti prezentovány současné poznatky o analýze, fyziologickém účinku a praktickém využití hypericinu a hyperforinu ve farmakochemii a biomedicínských oborech.
St. John's Wort (Hypericum perforatum L.) is commonly accepted as a medicinal plant. The data on the physiological activities of the individual substances that are produced in different organs of H. perforatum are well known at present. The highest attention is focused on the characterization and phytochemical properties of hypericin and hyperforin. These organic compounds are used as antidepressant, anticarcinogenic (photodynamic), antimicrobial and virostatic agents. The review paper surveys the present knowledge of chemical and analytical methods for their identification and quantification, physiological activity, and pharmacological and biomedical applications of hypericin and hyperforin.
- MeSH
- antitumorózní látky terapeutické užití MeSH
- depresivní poruchy farmakoterapie terapie MeSH
- lidé MeSH
- perylen analogy a deriváty izolace a purifikace terapeutické užití MeSH
- rostlinné extrakty terapeutické užití MeSH
- terpeny izolace a purifikace terapeutické užití MeSH
- třezalka chemie MeSH
- virové nemoci farmakoterapie terapie MeSH
- Check Tag
- lidé MeSH
