Critical illness induces among other events production of proinflammatory cytokines that in turn interfere with insulin signaling cascade and induce insulin resistance on a postreceptor level. Recently, local renin-angiotensin system of adipose tissue has been suggested as a possible contributor to the development of insulin resistance in patients with obesity. The aim of our study was to determine local changes of the renin-angiotensin system of subcutaneous and epicardial adipose tissue during a major cardiac surgery, which may serve as a model of an acute stress potentially affecting endocrine function of adipose tissue. Ten patients undergoing elective cardiac surgery were included into the study. Blood samples and samples of subcutaneous and epicardial adipose tissue were collected at the beginning and at the end of the surgery. Blood glucose, serum insulin and adiponectin levels were measured and mRNA for angiotensinogen, angiotensin-converting enzyme and angiotensin II type 1 receptor were determined in adipose tissue samples using RT PCR. Cardiac surgery significantly increased both insulin and blood glucose levels suggesting the development of insulin resistance, while serum adiponectin levels did not change. Expression of angiotensinogen mRNA significantly increased in epicardial adipose tissue at the end of surgery relative to baseline but remained unchanged in subcutaneous adipose tissue. Fat expression of angiotensin-converting enzyme and type 1 receptor for angiotensin II were not affected by surgery. Our study suggests that increased angiotensinogen production in epicardial adipose tissue may contribute to the development of postoperative insulin resistance.
- Keywords
- Adiponectin, Adipose tissue, Critical illness, Insulin resistance,
- MeSH
- Adiponectin blood MeSH
- Peptidyl-Dipeptidase A metabolism MeSH
- Angiotensinogen genetics metabolism MeSH
- Adult MeSH
- Elective Surgical Procedures MeSH
- Financing, Organized MeSH
- Insulin blood MeSH
- Insulin Resistance MeSH
- Cardiac Surgical Procedures adverse effects MeSH
- Blood Glucose metabolism MeSH
- Middle Aged MeSH
- Humans MeSH
- RNA, Messenger metabolism MeSH
- Pericardium metabolism MeSH
- Subcutaneous Fat metabolism MeSH
- Postoperative Complications etiology MeSH
- Receptor, Angiotensin, Type 1 metabolism MeSH
- Renin-Angiotensin System MeSH
- Aged MeSH
- Adipose Tissue metabolism MeSH
- Up-Regulation MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Aged MeSH
- Female MeSH
Genes for adiponectin and resistin are candidate genes of insulin resistance and type 2 diabetes mellitus. The aim of our study was to determine the frequency of single nucleotide polymorphisms (SNP) 45T>G and 276G>T of the adiponectin gene and 62G>A and -180C>G of the resistin gene in patients with obesity (OB), anorexia nervosa (AN) and in control healthy normal-weight women (NW) and to study the influence of particular genotypes on serum concentrations of these hormones and on insulin sensitivity. Serum adiponectin, resistin, tumor necrosis factor alpha (TNF-alpha), insulin, cholesterol, glycated hemoglobin (HbA1c) and blood glucose levels were measured in 77 patients with OB, 28 with AN and 38 NW. DNA analysis was carried out by polymerase chain reaction with restriction analysis of PCR product. The presence of SNP ADP+276 G>T allele was accompanied by higher cholesterol levels in AN patients, higher adiponectin concentrations in OB patients and lower HbA1c levels in NW. SNP of the resistin gene 62G>A was associated with lower HbA1c in NW and higher cholesterol concentrations in OB group. The carriers of the minor G allele in the position -180 of the resistin gene within AN group had significantly higher BMI relative to non-carriers. We conclude that polymorphisms in adiponectin and resistin genes can contribute to metabolic phenotype of patients with obesity and anorexia nervosa.
- Keywords
- Adiponectin, Polymorphism, Obesity, Anorexia Nervosa,
- MeSH
- Adiponectin genetics MeSH
- Cholesterol blood MeSH
- DNA biosynthesis genetics MeSH
- Adult MeSH
- Enzyme-Linked Immunosorbent Assay MeSH
- Phenotype MeSH
- Financing, Organized MeSH
- Glycated Hemoglobin metabolism MeSH
- Body Mass Index MeSH
- Polymorphism, Single Nucleotide MeSH
- Humans MeSH
- Anorexia genetics metabolism MeSH
- Obesity genetics metabolism MeSH
- Reverse Transcriptase Polymerase Chain Reaction MeSH
- Polymorphism, Restriction Fragment Length MeSH
- Polymorphism, Genetic physiology MeSH
- Resistin genetics MeSH
- Tumor Necrosis Factor-alpha blood MeSH
- Check Tag
- Adult MeSH
- Humans MeSH
- Female MeSH
The aim of the present study was to evaluate the expression profile of genes potentially related to metabolic complications of obesity in the whole adipose tissue and isolated adipocytes from subcutaneous (SAT) and visceral adipose tissue (VAT) from 12 non-diabetic obese women and 12 lean women. Real-time polymerase chain reaction was used for expression analysis of 41 genes of interest and two housekeeping genes. We found increased expression of specific proinflammatory and adipogenic genes and reduced expression of specific lipogenic and insulin signaling pathway genes in obese relative to lean women with no preferable localization in SAT or VAT depot. The gene expression significantly differed between adipocytes and adipose tissue but both contributed to the proinflammatory profile in obesity. We conclude that both SAT and VAT exhibit alterations in the expression of specific genes possibly contributing to proinflammatory and insulin resistance state and consequently to metabolic complications of obesity.
- MeSH
- Adult MeSH
- Financing, Organized MeSH
- Middle Aged MeSH
- Humans MeSH
- Microarray Analysis MeSH
- Intra-Abdominal Fat physiology MeSH
- Obesity metabolism physiopathology MeSH
- Subcutaneous Fat physiology MeSH
- Gene Expression Profiling MeSH
- Adipocytes cytology physiology MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Female MeSH
AIM: Comparison of manual and automatic (MagNA Pure) isolation methods of total RNA from adipose tissue with respect to its quality and recovery factor.
- MeSH
- Financing, Organized MeSH
- DNA, Complementary genetics MeSH
- Leptin genetics MeSH
- Humans MeSH
- Molecular Biology methods MeSH
- Reverse Transcriptase Polymerase Chain Reaction MeSH
- RNA isolation & purification MeSH
- Adipose Tissue metabolism MeSH
- Check Tag
- Humans MeSH
- Publication type
- Comparative Study MeSH
OBJECTIVE: To study the influence of chronic malnutrition in patients with anorexia nervosa on endocrine function of adipose tissue on both circulating and subcutaneous fat mRNA expression level. PATIENTS AND DESIGN: A total of 12 patients with anorexia nervosa and 18 normal weight age-matched women underwent anthropometric examination, single blood drawing and subcutaneous adipose tissue biopsy. MEASUREMENTS: Serum concentrations of high-sensitive CRP (hsCRP), leptin, soluble leptin receptor, adiponectin, resistin, interleukin-6 and insulin were measured by Luminex, enzyme-linked immunosorbent assay (ELISA) and radioimmunoassay (RIA) kits. Subcutaneous adipose tissue mRNA expression of the same adipokines, adiponectin receptors 1 and 2 and immunocompetent cells marker CD68 was measured by real-time polymerase chain reaction (PCR). RESULTS: Decreased body fat content of patients with anorexia nervosa was accompanied by reduced hsCRP, leptin and increased adiponectin and soluble leptin receptor. Resistin, interleukin-6 and insulin levels did not differ from those of the control group. Fat mRNA adiponectin, leptin, interleukin-6 and CD68 expression was reduced, resistin mRNA expression was increased and adiponectin receptor 1 and 2 expression were unchanged as compared to the control group. CONCLUSIONS: Local perturbations in resistin, adiponectin and interleukin-6 mRNA expression in subcutaneous adipose tissue are not reflected by its circulating levels. These changes could be involved in some local metabolic disturbances in subcutaneous adipose tissue of anorexia nervosa patients.
- MeSH
- Adiponectin genetics blood MeSH
- Antigens, Differentiation, Myelomonocytic genetics MeSH
- C-Reactive Protein analysis MeSH
- Antigens, CD genetics MeSH
- Adult MeSH
- Gene Expression MeSH
- Financing, Organized MeSH
- Adaptation, Physiological MeSH
- Interleukin-6 genetics MeSH
- Insulin blood MeSH
- Blood Glucose analysis MeSH
- Receptors, Leptin blood MeSH
- Humans MeSH
- Anorexia Nervosa immunology metabolism MeSH
- RNA, Messenger analysis MeSH
- Statistics, Nonparametric MeSH
- Paracrine Communication MeSH
- Subcutaneous Fat chemistry immunology metabolism MeSH
- Malnutrition immunology metabolism MeSH
- Resistin genetics blood MeSH
- Case-Control Studies MeSH
- Check Tag
- Adult MeSH
- Humans MeSH
- Female MeSH
- Publication type
- Comparative Study MeSH
