OBJECTIVE: The number of patients with end-stage renal disease (ESRD) is rising and these patients are at higher risk of cardiovascular disease. We studied the role of hormonal production of adipose tissue in the development of chronic inflammation in patients with ESRD before kidney transplantation. METHODS: Fifteen women with ESRD and 17 healthy women (control) underwent single blood drawing and visceral and subcutaneous adipose tissue sampling during surgery (kidney transplantation in the ESRD group or cholecystectomy in the control group). Serum concentrations of C-reactive protein, interleukin-6, tumor necrosis factor-alpha, leptin, adiponectin, resistin, monocyte chemoattractant protein-1 were measured. Messenger RNA expression of the same hormones, adiponectin receptors 1 and 2 and immunocompetent cell marker CD68 in subcutaneous and visceral samples were measured using real-time polymerase chain reaction. Adipose tissue was examined immunohistochemically for CD68-positive cells. RESULTS: Serum concentrations of C-reactive protein, adiponectin, resistin, interleukin-6, tumor necrosis factor-alpha, and monocyte chemoattractant protein-1 were significantly higher in the ESRD versus control group. Subcutaneous and visceral mRNA expressions of tumor necrosis factor-alpha and CD68 were significantly increased in the ESRD versus control group. Adiponectin receptor-1 and monocyte chemoattractant protein-1 mRNA expressions were significantly higher in visceral but not in subcutaneous adipose tissue of the ESRD group. Messenger RNA expressions of resistin, leptin, adiponectin, interleukin-6, and adiponectin receptor-2 in both fat depots did not significantly differ between groups. Increased infiltration of subcutaneous and visceral adipose tissue with CD68-positive immunocompetent cells was found in the ESRD group by histologic examination. CONCLUSION: Subcutaneous and visceral adipose tissues in ESRD express higher amounts of proinflammatory cytokines and may play a role in the development of systemic inflammation.
- MeSH
- adiponektin genetika metabolismus MeSH
- antigeny diferenciační myelomonocytární metabolismus MeSH
- C-reaktivní protein metabolismus MeSH
- CD antigeny metabolismus MeSH
- chemokin CCL2 genetika metabolismus MeSH
- chronické selhání ledvin metabolismus MeSH
- cytokiny metabolismus MeSH
- interleukin-6 genetika metabolismus MeSH
- lidé středního věku MeSH
- lidé MeSH
- mediátory zánětu metabolismus MeSH
- messenger RNA biosyntéza MeSH
- nitrobřišní tuk metabolismus MeSH
- podkožní tuk metabolismus MeSH
- receptory adiponektinu biosyntéza genetika MeSH
- resistin genetika metabolismus MeSH
- TNF-alfa krev MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- práce podpořená grantem MeSH
Genes for adiponectin and resistin are candidate genes of insulin resistance and type 2 diabetes mellitus. The aim of our study was to determine the frequency of single nucleotide polymorphisms (SNP) 45T>G and 276G>T of the adiponectin gene and 62G>A and -180C>G of the resistin gene in patients with obesity (OB), anorexia nervosa (AN) and in control healthy normal-weight women (NW) and to study the influence of particular genotypes on serum concentrations of these hormones and on insulin sensitivity. Serum adiponectin, resistin, tumor necrosis factor alpha (TNF-alpha), insulin, cholesterol, glycated hemoglobin (HbA1c) and blood glucose levels were measured in 77 patients with OB, 28 with AN and 38 NW. DNA analysis was carried out by polymerase chain reaction with restriction analysis of PCR product. The presence of SNP ADP+276 G>T allele was accompanied by higher cholesterol levels in AN patients, higher adiponectin concentrations in OB patients and lower HbA1c levels in NW. SNP of the resistin gene 62G>A was associated with lower HbA1c in NW and higher cholesterol concentrations in OB group. The carriers of the minor G allele in the position -180 of the resistin gene within AN group had significantly higher BMI relative to non-carriers. We conclude that polymorphisms in adiponectin and resistin genes can contribute to metabolic phenotype of patients with obesity and anorexia nervosa.
- Klíčová slova
- Adiponectin, Polymorphism, Obesity, Anorexia Nervosa,
- MeSH
- adiponektin genetika MeSH
- cholesterol krev MeSH
- DNA biosyntéza genetika MeSH
- dospělí MeSH
- ELISA MeSH
- fenotyp MeSH
- financování organizované MeSH
- glykovaný hemoglobin metabolismus MeSH
- index tělesné hmotnosti MeSH
- jednonukleotidový polymorfismus MeSH
- lidé MeSH
- nechutenství genetika metabolismus MeSH
- obezita genetika metabolismus MeSH
- polymerázová řetězová reakce s reverzní transkripcí MeSH
- polymorfismus délky restrikčních fragmentů MeSH
- polymorfismus genetický fyziologie MeSH
- resistin genetika MeSH
- TNF-alfa krev MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- ženské pohlaví MeSH
AIM: Comparison of manual and automatic (MagNA Pure) isolation methods of total RNA from adipose tissue with respect to its quality and recovery factor.
- MeSH
- financování organizované MeSH
- komplementární DNA genetika MeSH
- leptin genetika MeSH
- lidé MeSH
- molekulární biologie metody MeSH
- polymerázová řetězová reakce s reverzní transkripcí MeSH
- RNA izolace a purifikace MeSH
- tuková tkáň metabolismus MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- srovnávací studie MeSH
- MeSH
- adiponektin genetika krev metabolismus MeSH
- finanční podpora výzkumu jako téma MeSH
- index tělesné hmotnosti MeSH
- inzulinová rezistence MeSH
- lidé MeSH
- messenger RNA MeSH
- obezita metabolismus MeSH
- podkožní tuk metabolismus MeSH
- receptory peptidů MeSH
- regulace genové exprese MeSH
- vztahy mezi strukturou a aktivitou MeSH
- Check Tag
- lidé MeSH
- ženské pohlaví MeSH
