• MeSH
• nemoci ucha MeSH
• otitis media chirurgie   MeSH
• střední ucho MeSH
• tympanoplastika MeSH

• Konspekt
• Patologie. Klinická medicína
• NLK Obory
• otorinolaryngologie

Pulsatile Carmat bioprosthetic total artificial heart (C-TAH) is designed to be implanted in patients with biventricular end-stage heart failure. Since flow variation might contribute to endothelial dysfunction, we explored circulating endothelial biomarkers after C-TAH implantation in seven patients and compared the manual and autoregulated mode. Markers of endothelial dysfunction and regeneration were compared before and during a 6- to 9-month follow-up after implantation. The follow-up was divided into three periods (< 3, 3-6, and > 6 months) and used to estimate the temporal trends during the study period. A linear mixed model was used to analyze repeated measures and association between tested parameters according to the mode of C-TAH and the time. Relevance of soluble endoglin (sEndoglin) level increase has been tested on differentiation and migration potential of human vasculogenic progenitor cells (endothelial colony forming cells [ECFCs]). Normal sEndoglin and soluble endothelial protein C receptor (sEPCR) levels were found in patients after implantation with autoregulated C-TAH, whereas they significantly increased in the manual mode, as compared with pretransplant values (p = 0.005 and 0.001, respectively). In the autoregulated mode, a significant increase in the mobilization of cytokine stromal cell-derived factor 1 was found (p = 0.03). After adjustment on the mode of C-TAH, creatinine or C-reactive protein level, sEndoglin, and sEPCR, were found significantly associated with plasma total protein levels. Moreover, a significant decrease in pseudotubes formation and migration ability was observed in vitro in ECFCs receiving sEndoglin activation. Our combined analysis of endothelial biomarkers confirms the favorable impact of blood flow variation achieved with autoregulation in patients implanted with the bioprosthetic total artificial heart.

• MeSH
• biologické markery analýza   MeSH
• bioprotézy * MeSH
• endoglin analýza   MeSH
• endotel patologie   MeSH
• endoteliální receptor proteinu C analýza   MeSH
• homeostáza MeSH
• lidé MeSH
• následné studie MeSH
• senioři MeSH
• srdeční selhání terapie   MeSH
• umělé srdce * MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• Publikační typ
• časopisecké články MeSH

BACKGROUND: The study aimed at finding a laboratory approach to detect endothelial damage in normal pregnancy as well as in pregnancy complicated by preeclampsia using selected markers of endothelial activation. MATERIALS: A total of 403 healthy pregnant women without a history of deep vein thrombosis and/or hypertension were prospectively studied. From all women, venous blood was collected before the end of the 1st trimester, between weeks 24 and 28 of gestation, and in the 3rd trimester (weeks 34-36). Assays of tissue plasminogen activator, plasminogen activator inhibitor-1, von Willebrand factor activity and antigen, thrombomodulin, endothelial protein C receptor, and endothelial microparticles activated by TF were performed. RESULTS: When comparing women who developed preeclampsia during pregnancy (the average levels were 23.41 mug/L, 34.33 mug/L, and 53.56 mug/L in the 1st, 2nd, and 3rd trimesters, respectively) with healthy pregnant women (the average levels were 19.05 mug/L, 28.47 mug/L, and 39.86 mug/L in the 1st, 2nd, and 3rd trimesters, respectively) significant differences in the levels of thrombomodulin were found in all three trimesters. By contrast, no statistically significant differences in the levels of vWF (both antigen and activity), t-PA, EPCR, EMPs, MMP-2, MMP-9, and TIMP-9 were found in any trimesters in the same group. CONCLUSIONS: Pregnancy and preeclampsia strongly influence the levels of studied markers. The findings of this work confirm the possible predictive potential of thrombomodulin and PA-1.

• MeSH
• biologické markery * krev   MeSH
• CD antigeny krev   MeSH
• cévní endotel patofyziologie   MeSH
• dospělí MeSH
• endoteliální receptor proteinu C MeSH
• inhibitor aktivátoru plazminogenu 1 krev   MeSH
• lidé MeSH
• mladý dospělý MeSH
• preeklampsie * krev   patofyziologie   MeSH
• prospektivní studie MeSH
• první trimestr těhotenství * krev   MeSH
• receptory buněčného povrchu krev   MeSH
• referenční hodnoty MeSH
• studie případů a kontrol MeSH
• těhotenství MeSH
• tkáňový aktivátor plazminogenu krev   MeSH
• trombomodulin krev   MeSH
• von Willebrandův faktor metabolismus   MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mladý dospělý MeSH
• těhotenství MeSH
• ženské pohlaví MeSH

Emergency granulopoiesis is the enhanced and accelerated production of granulocytes that occurs during acute infection. The contribution of hematopoietic stem cells (HSCs) to this process was reported; however, how HSCs participate in emergency granulopoiesis remains elusive. Here, using a mouse model of emergency granulopoiesis we observe transcriptional changes in HSCs as early as 4 h after lipopolysaccharide (LPS) administration. We observe that the HSC identity is changed towards a myeloid-biased HSC and show that CD201 is enriched in lymphoid-biased HSCs. While CD201 expression under steady-state conditions reveals a lymphoid bias, under emergency granulopoiesis loss of CD201 marks the lymphoid-to-myeloid transcriptional switch. Mechanistically, we determine that lymphoid-biased CD201+ HSCs act as a first response during emergency granulopoiesis due to direct sensing of LPS by TLR4 and downstream activation of NF-κΒ signaling. The myeloid-biased CD201- HSC population responds indirectly during an acute infection by sensing G-CSF, increasing STAT3 phosphorylation, and upregulating LAP/LAP* C/EBPβ isoforms. In conclusion, HSC subpopulations support early phases of emergency granulopoiesis due to their transcriptional rewiring from a lymphoid-biased to myeloid-biased population and thus establishing alternative paths to supply elevated numbers of granulocytes.

• MeSH
• granulocyty metabolismus   MeSH
• hematopoetické kmenové buňky * MeSH
• hematopoéza MeSH
• lipopolysacharidy * metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH

OBJECTIVES: It is suggested that delivery whether spontaneous or by elective cesarean section is associated with an inflammatory reaction which may be modified by the type of delivery. Inflammatory reactions are associated with endothelial activation. The aim of our study was to assess endothelial biomarkers in cord and neonatal blood following different modes of delivery. STUDY DESIGN: The study group consisted of term healthy newborns after uncomplicated pregnancies and either spontaneous vaginal delivery (n = 39) or elective cesarean section (n = 20). Plasma soluble biomarkers were measured using multiplex magnetic bead immunoassay. The microvesicle count and number of surface antigen-specific microvesicles were determined by flow cytometry. RESULTS: We found significantly increased concentrations of cord blood endothelial markers (sVEGFR1, Endothelin-1 and sVCAM1) and microvesicles (EPCR/CD201+, ICAM1/CD54+ and PECAM1/CD31+) in spontaneous vaginal delivery when compared to elective cesarean section. Irrespective of the delivery mode endothelial markers sVEGFR1, Endocan, Angiopoietin-2, VEGF, and sICAM1, were significantly increased in neonatal compared to cord blood. CONCLUSION: We found increased cord blood concentrations of endothelial markers and microvesicles following spontaneous vaginal delivery, which may reflect the natural activation of endothelial cells during labor. Following the delivery, most of the soluble markers increased, as a possible consequence of activation of neonatal innate immunity and postnatal cardiovascular transition.

• MeSH
• císařský řez * MeSH
• endoteliální buňky MeSH
• fetální krev MeSH
• lidé MeSH
• novorozenec MeSH
• porodní děj * MeSH
• těhotenství MeSH
• vedení porodu MeSH
• Check Tag
• lidé MeSH
• novorozenec MeSH
• těhotenství MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH

Úvod: Akútna mozgovocievna príhoda predstavuje závažný stav ako pre pacienta, tak i lekára. Nepriaznivé reziduálne následky prinášajú problémy ako pre neho samotného, tak i jeho rodinu i pre spoločnosť, liečba je aj ekonomicky náročná. Pacienti a metodika: Sledovaných bolo 50 následných pacientov s akútnou mozgovocievnou príhodou, ktorí boli prijatí na akútnu intenzívnu starostlivosť na internej klinike. Počas akútnej hospitalizácie bolo realizované aj vyšetrenie lipidových parametrov metódou Lipoprint. Stanovenie veľkosti LDL partikúl (LDL 1–7) bolo realizované nalačno, ešte pred zahájením medikamentóznej liečby. Výsledky: Aterogénny lipidový profil bol detekovaný u viac než 3/4 sledovaných pacientov s akútnou mozgovocievnou príhodou v porovnaní s kontrolnou skupinou. Prítomnosť malých denzných LDL partikúl sa významne líšila u pacientov s mozgovocievnou príhodou oproti zdravým osobám. Záver: Včasná hypo­lipidemická liečba je indikovaná u pacientov s náhlou mozgovocievnou príhodou na dosiahnutie zníženia ich vysokého globálneho kardio­vaskulárneho rizika.

Introduction: Acute stroke represents the severe state both for the patient as well as for the physician. Poor outcome with residual handicap brings the problems both for the family and for the society and the therapy and follow-up is cost expensive. Patients and methods: 50 consecutive patients with acute stroke were admitted to the acute care unit at the internal medicine department. During acute care blood sample was examined with Lipoprint method to evaluate their lipid parameters. Size of LDL particles spectrum (LDL 1–7), before starting the drug treatment were measured. Results: Atherogenic profile was detected in more than three quarters of the stroke patients comparing to the controls. Presence of atherogenic smalldense LDL particles significantly differs among stroke patients to the healthy controls. Conclusion: Early hypolipidemic treatment among patients with acute stroke is recommended in order to lower their high cardiovascular risk.

• MeSH
• cévní mozková příhoda * epidemiologie   etiologie   MeSH
• dospělí MeSH
• dyslipidemie * epidemiologie   farmakoterapie   prevence a kontrola   MeSH
• hodnocení rizik MeSH
• hypolipidemika terapeutické užití   MeSH
• LDL-cholesterol * analýza   MeSH
• lidé středního věku MeSH
• lidé MeSH
• rizikové faktory * MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• ženské pohlaví MeSH