... Combining Laser Scanning Confocal Microscopy Xuejun Sun and Electron Microscopy -- 10. ... ... Three-Dimensional Visualization of Cytoskeleton Werner Baschong, by Confocal Laser Scanning Microscopy ... ... Visualization of Nuclear Pore Complex and Its Dis- Ulrich Kubitscheck, tribution by Confocal Laser Scanning ... ... Observation of Microcirculatory Kinetics by Real-Time Confocal Laser Scanning Microscopy -- Kazuhiro ... ... Video-Rate, Scanning Slit Confocal Microscopy of Living Human Cornea in Vivo: Three-Dimensional Confocal ...
Methods in enzymology ; Vol. 307
[1st ed.] xxxii, 663 s. : il.
- MeSH
- Microscopy, Confocal MeSH
- Conspectus
- Patologie. Klinická medicína
- NML Fields
- chemie, klinická chemie
Mezi hlavni přednosti konfokální mikroskopie ve srovnaní s klasickou optickou mikroskopii patři možnost zaznamenávat série digitalizovaných obrazů relativně tenkých optických řezů vedených i tlustším vzorkem. V článkuje ukázáno využití těchto sérií obrazů, jak při vizualizaci, tak i při kvantifikaci trojrozměrných objektů: Jsou popsány různé typy rekonstrukcí objektů, které lze proostřit konfokálním mikroskopem, i měření jejich geometrických charakteristik s využitím stereologických metod. Podrobněji je přiblížena stereologická metoda užívající virtuální fakirské sondy pro odhad objemu i povrchu studovaného objektu.
The possibility to record series of digitized images of relatively thin optical sections within a thick speciment is one of the main advantages ofconfocal mircoscopy if compared with the conventional optical microscopy. It is shown how such series can be exploited in visualization and quantification of three-dimensional objects: different types of reconstructions of objects that can be focussed through by a confocal microscope as well as the estimation of their geometrical characteristics by stereological methods are described. The stereological method using virtual fakir probes for volume and surface area estimation is presented in detail.
- MeSH
- Microscopy, Confocal MeSH
- Humans MeSH
- Software MeSH
- Computational Biology MeSH
- Check Tag
- Humans MeSH
Methods in molecular biology ; vol. 122
xi, 446 s. : il.
106 s.
- MeSH
- Barrett Esophagus diagnosis prevention & control MeSH
- Biopsy methods utilization MeSH
- Endoscopy, Digestive System history methods utilization MeSH
- Financing, Organized utilization MeSH
- Fluorescein diagnostic use MeSH
- Microscopy, Fluorescence methods utilization MeSH
- Colorectal Neoplasms diagnosis prevention & control MeSH
- Microscopy, Confocal methods utilization MeSH
- Stomach Neoplasms diagnosis prevention & control MeSH
- Geographicals
- Czech Republic MeSH
Taxonomic issues within Trypanorhyncha, e.g., the inaccurate light microscopic visualisation of the hook patterns, are solvable by confocal laser scanning microscopy (CLSM). We applied CLSM imaging to study Trygonicola macropora (Shipley et Hornell, 1906) and Dollfusiella michiae (Southwell, 1929) from Neotrygon caeruliopunctata Last, White et Séret from Bali, Indonesia. To illustrate the strength and limitations of CLSM, images of Otobothrium cysticum (Mayer, 1842) and Symbothriorhynchus tigaminacantha Palm, 2004, both permanent mounts from a collection, were also processed. The CLSM created image stacks of many layers, and edited with IMARIS Software, these layers resulted in three-dimensional images of the armature patterns and internal organs of both species. BABB (benzylalcohol and benzylbenzuolate) clearing was applied to T. macropora. We conclude that trypanorhynch cestodes stained with Mayer-Schuberg's acetic carmine permanently mounted in Canada balsam are suitable for CLSM, allowing detailed analyses of museum type-material as well as freshly collected and processed worms. BABB resulted in imaging the testes in detail, suggesting other stains to be used for CLSM in trypanorhynch cestode research. Application of CLSM for studies of other cestode groups is highly recommended.
- MeSH
- Cestoda * MeSH
- Microscopy, Confocal * MeSH
- Museums MeSH
- Platyhelminths * MeSH
- Skates, Fish * MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Keywords
- laserová skenovací konfokální mikroskopie (CLSM, LSCM), reflexní konfokální mikroskopie (RCM), neinvazívní in vivo vyšetření,
- MeSH
- Keratosis, Actinic diagnosis MeSH
- Carcinoma, Basal Cell diagnosis MeSH
- Bowen's Disease diagnosis MeSH
- Dermatology * methods MeSH
- Microscopy, Confocal * history methods trends utilization MeSH
- Dermatitis, Contact diagnosis MeSH
- Laser Therapy MeSH
- Humans MeSH
- Melanoma diagnosis MeSH
- Carcinoma, Squamous Cell diagnosis MeSH
- Skin Aging drug effects radiation effects MeSH
- Check Tag
- Humans MeSH
- Publication type
- Research Support, Non-U.S. Gov't MeSH
