elektronický časopis
- MeSH
- plicní hypertenze MeSH
- Konspekt
- Patologie. Klinická medicína
- NLK Obory
- pneumologie a ftizeologie
- NLK Publikační typ
- elektronické časopisy
- MeSH
- hydrokortison MeSH
- imunologické techniky MeSH
- imunosupresivní léčba MeSH
- myši MeSH
- slezina cytologie MeSH
- Check Tag
- myši MeSH
- MeSH
- imunogenicita vakcíny MeSH
- lidé MeSH
- pneumokokové vakcíny * imunologie škodlivé účinky MeSH
- systémový lupus erythematodes * imunologie MeSH
- vakcinace MeSH
- vakcíny proti chřipce imunologie škodlivé účinky MeSH
- vakcíny proti papilomavirům imunologie škodlivé účinky MeSH
- virové vakcíny * imunologie škodlivé účinky MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- přehledy MeSH
Many bacteria are capable of accumulating intracellular granules of polyhydroxyalkanoates (PHA). In this work, we developed confocal microscopy analysis of bacterial cells to study changes in the diameters of cells as well as PHA granules during growth and PHA accumulation in the bacterium Cupriavidus necator H16 (formerly Ralstonia eutropha). The cell envelope was stained by DiD(®) fluorescent probe and PHA granules by Nile Red. Signals from both probes were separated based on their spectral and fluorescence life-time properties. During growth and PHA accumulation, bacterial cells increased their length but the width of the cells remained constant. The volume fraction of PHA granules in cells increased during PHA accumulation, nevertheless, its value did not exceed 40 vol. % regardless of the PHA weight content. It seems that bacterial cultures lengthen the cells in order to control the PHA volume portion. However, since similar changes in cell length were also observed in a PHA non-accumulating mutant, it seems that there is no direct control mechanism, which regulates the prolongation of the cells with respect to PHA granules volume. It is more likely that PHA biosynthesis and the length of cells are influenced by the same external stimuli such as nutrient limitation.
- MeSH
- Cupriavidus necator růst a vývoj metabolismus ultrastruktura MeSH
- cytoplazmatická granula metabolismus ultrastruktura MeSH
- fluorescenční mikroskopie MeSH
- konfokální mikroskopie MeSH
- polyhydroxyalkanoáty chemie metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
The aim of this work was to investigate the thermophilic bacterium Schelegelella thermodepolymerans DSM 15344 in terms of its polyhydroxyalkanoates (PHA) biosynthesis capacity. The bacterium is capable of converting various sugars into PHA with the optimal growth temperature of 55 °C; therefore, the process of PHA biosynthesis could be robust against contamination. Surprisingly, the highest yield was gained on xylose. Results suggested that S. thermodepolymerans possess unique xylose metabolism since xylose is utilized preferentially with the highest consumption rate as compared to other sugars. In the genome of S. thermodepolymerans DSM 15344, a unique putative xyl operon consisting of genes responsible for xylose utilization and also for its transport was identified, which is a unique feature among PHA producers. The bacterium is capable of biosynthesis of copolymers containing 3-hydroxybutyrate and also 3-hydroxyvalerate subunits. Hence, S.thermodepolymerans seems to be promising candidate for PHA production from xylose rich substrates.
- MeSH
- Comamonadaceae * MeSH
- kyselina 3-hydroxymáselná MeSH
- polyhydroxyalkanoáty * MeSH
- xylosa MeSH
- Publikační typ
- časopisecké články MeSH
