Q112413334
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105 s. : obr.
Acta hygienica, epidemiologica et microbiologica, ISSN 0862-5956 Příloha č.2/1991
36 s. : tab. ; 21 cm
- MeSH
- adjuvancia imunologická aplikace a dávkování MeSH
- bakteriální infekce imunologie prevence a kontrola MeSH
- bakteriální vakcíny aplikace a dávkování klasifikace terapeutické užití MeSH
- bakteriologické techniky metody normy využití MeSH
- příprava léků metody normy MeSH
- Publikační typ
- směrnice MeSH
- Konspekt
- Mikrobiologie
- NLK Obory
- alergologie a imunologie
- mikrobiologie, lékařská mikrobiologie
- bakteriologie
Study objectives: To detect biofilm formation in Staphylococcus aureus strains and to determine the minimal biofilm inhibition concentrations (MBIC) and the minimal biofilm eradicating concentrations (MBEC) of vancomycin, gentamicin and rifampin. To compare the MBIC and MBEC with the minimal inhibition concentration (MIC) and minimal bactericidal concentration (MBC) data for planktonic Staphylococcus aureus forms that are commonly used in antimicrobial susceptibility testing for the purposes of individualized therapy. Patients and Methods: Fifteen S. aureus strains isolated from central venous catheters, intratracheal tubes and wound drainage tubes from the patients of the University Hospital, Bratislava-Staré Mesto were included in the study. Selected virulence factors were characterized. The biofilm formation potential was measured by a modified crystal violet micro-assay. The presence of viable cells biofilm in was tested using 3-(4,5-dimethylthiazol- -2-yl)-2,5-diphenyl tetrazolium bromide (MTT). The MIC and MBC of vancomycin, gentamicin and rifampin was tested in planktonic S. aureus forms by the broth microdilution method. The MBIC and MBEC of these antimicrobial drugs for biofilm S. aureus forms were determined by a modified microdilution method. Student’s t-test was used for statistical analysis of the results. Results: All of the study strains formed biofilm, with only two of them having a low biofilm formation potential. MTT revealed moderate to high metabolic activity of bacteria biofilm in Vancomycin MICs and MBICs were identical in 80 % of the study strains. Vancomycin MBECs are higher than MBCs in all the study strains, are interpreted as resistance according to the criteria of the Clinical and Laboratory Standards Institute (CLSI) and make the drug unsuitable for use in the treatment. In vitro gentamicin MBICs indicated susceptibility according to the CLSI criteria but gentamicin MBECs were interpreted as gentamicin resistance. Rifampin MICs and MBICs of the study strains revealed susceptibility. Rifampin MBCs were interpreted as susceptibility, but based on MBECs, 13 % of the study strains were considered as resistant and 13 % of the study strains showed intermediate susceptibility. The differences between gentamicin and rifampin MICs and MBICs and those between MBCs and MBECs of all antimicrobials tested were statistically significant. Conclusion: The tested biofilm S. aureus forms showed high MBECs of vancomycin, gentamicin and rifampin, with rifampin only being suitable for therapeutic use. To provide reliable results for individualized antibiotic therapy, it will be needed to test in vitro biofilm formation, to determine MBIC and MBEC of antimicrobial drugs using a standardized method, to interpret the test results in relation to biofilm S. aureus forms and to establish the interpretation criteria for MBIC and MBEC similarly to MIC and MBC.
- Klíčová slova
- S. aureus, biofilm, citlivosť na antimikrobiálne liečivá, gentamicín, rifampicín,
- MeSH
- antibakteriální látky farmakologie MeSH
- biofilmy účinky léků MeSH
- gentamiciny farmakologie MeSH
- mikrobiální testy citlivosti MeSH
- rifampin farmakologie MeSH
- Staphylococcus aureus fyziologie účinky léků MeSH
- vankomycin farmakologie MeSH
- MeSH
- antibiotická rezistence genetika imunologie MeSH
- homoserin analýza imunologie MeSH
- laktony analýza imunologie MeSH
- lidé MeSH
- oxidační stres imunologie účinky léků účinky záření MeSH
- pseudomonádové infekce imunologie komplikace MeSH
- Pseudomonas aeruginosa imunologie izolace a purifikace patogenita MeSH
- techniky in vitro MeSH
- Check Tag
- lidé MeSH
- MeSH
- bakteriální toxiny izolace a purifikace škodlivé účinky MeSH
- Escherichia coli genetika izolace a purifikace patogenita MeSH
- finanční podpora výzkumu jako téma MeSH
- hemolyticko-uremický syndrom etiologie mikrobiologie MeSH
- infekce vyvolané Escherichia coli diagnóza komplikace MeSH
- lidé MeSH
- regulace genové exprese u bakterií MeSH
- Check Tag
- lidé MeSH
- MeSH
- epidemický výskyt choroby MeSH
- Escherichia coli O157 izolace a purifikace MeSH
- finanční podpora výzkumu jako téma MeSH
- hemolyticko-uremický syndrom epidemiologie MeSH
- kolitida epidemiologie MeSH
- lidé MeSH
- mléko mikrobiologie MeSH
- shiga toxiny metabolismus MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Geografické názvy
- Slovenská republika MeSH
- MeSH
- bakteriální toxiny biosyntéza MeSH
- dítě MeSH
- Escherichia coli enzymologie MeSH
- hemolyticko-uremický syndrom etiologie MeSH
- lidé MeSH
- shiga toxin biosyntéza MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- Publikační typ
- kongresy MeSH
Cieľ práce: Od roku 1980 sa stali infekcie kmeňmi Escherichia coli produkujúcimi shiga toxíny (STEČ) veľmi sledovanými nielen mikrobiológmi ale aj širokou verejnosťou. STEČ sú asociované so závažným a často aj fatálne prebiehajúcim hemolyticko-uremickým syndrómom. Najdôležitejšiu úlohu v patogeneze tohoto ochorenia zohráva uvoľnenie shiga toxínov (Stx) do cirkulácie po prieniku cez črevnú stenu. STEČ získa najčastejšie pacient požitím kontaminovanej potravy. Autori opisujú rodinný výskyt hemolyticko-uremického syndrómu (HUS) spôsobeného kmeňom E. coli O157. Zo štrnástich členov rodiny, bola u deviatich členov detekovaná E. coli O157 v stolici, v troch prípadoch došlo k rozvoju HUS. Materiál a metódy: Bolo vyšetřených 69 vzoriek: 54 stolíc, 1 vzorka kozího mUeka a kozího trusu, 1 vzorka domácky vyrobenej detskej výživy, 7 vzoriek nepasterizovaného kravského mheka a 5 vzoriek kravského trusu. Na izoláciu STEČ a detekciu vybraných faktorov virulencie bola použitá selektívna kultivácia, imunomagnetická separácia, latexová aglutinácia a multiplexná PCR. Výsledky: Izolovali sme 14 kmeňov E. coli O157, ktoré nefermentovali sorbitol. Izolovali sme ich od pacientov s HUS (n = 3), s hemoragickou kolitidou (HC) (n = 2), od asymptomatických nosičov (n = 4), z kravského trusu (n = 4) a z kravského mlieka (n = 1). Všetky izolované kmene mali gén pre shiga toxin 2, intimín a enterohemolyzín. U vrtkých kmeňov sme dokázali produkciu Stx2. Záver. Autori detekovali prvýkrát na Slovensku STEČ produkujúce Stx2, ktoré vyvolali HUS. Autorom sa podarilo dokázať aj zdroj infekcie, kterým bolo kontaminované nepasterizované mlieko použité pri príprave pudingu.
Purpose of the study. Since 1980 both microbiologists and the general public are paying increasingly more attention to infections by the serotypes of Escherichia coli that produce shiga toxins (STEC). STECs are associated with the serious and often fatal haemolytic uraemic syndrome. Crucial in the pathogenesis of this disease is the release, through the intestinal wall, of shiga toxins (Stx) into blood circulation. The most frequent source of STECs is contaminated food. The authors describe the family incidence of the haemolytic uraemic syndrome (HUS) caused by the E. coli serotype O157. E. coli O157 was detected in the faeces of nine out of fourteen family members. HUS developed in three cases. Material and methods: 69 samples were analysed: 54 faeces samples, 1 sample of goat's milk and of goat's dung, 1 sample of home-made baby food, 7 samples of non-pasteurized cow's milk and 7 samples of cow's dung. Selective cultivation, immunomagnetic separation, latex agglutination and multiplex PCR were used to isolate STECs and to detect selected virulence factors. Results: We isolated 14 E. coli serotypes O157 that did not ferment sorbitol. They were isolated from patients presenting HUS (n = 3) and haemorrhagic colitis (HC) (n = 2), from asymptomatic carriers (n = 4), from cow's dung (n = 4) and from cow's milk (n = 1). All the isolated serotypes presented a gene for shiga toxin 2, intimin and enterohaemolysin. In all serotypes we demonstrated the production ofStx2. Conclusions: The authors detected, for the first time in Slovakia, STECs producing Stx2 that cause HUS. They succeeded in tracing the source of the infection -contaminated non-pasteurized milk used to make a pudding.
- MeSH
- bakteriologické techniky metody MeSH
- Escherichia coli O157 patogenita MeSH
- finanční podpora výzkumu jako téma MeSH
- hemolyticko-uremický syndrom diagnóza patologie MeSH
- imunologické techniky metody MeSH
- infekce vyvolané Escherichia coli diagnóza patologie MeSH
- lidé MeSH
- shiga toxin 2 MeSH
- zdraví rodiny MeSH
- Check Tag
- lidé MeSH
