Strain-differences
Dotaz
Zobrazit nápovědu
Stroke is despite of progressive improvements in treatment and reperfusion strategies one of the most devastating human pathology. However, as quality of acute health care improves and more people survive ischemic attack, healthcare specialists have to solve new challenges to preserve reasonable quality of life to these patients. Thus, novel approaches which prevents comorbidities of stroke and improve quality of life of stroke survivors in general has to be developed and experimentally tested. The aim of the present paper was to establish reliable rat model of middle cerebral occlusion and set of methods allowing selection of animals suitable for long-term experiments. We have compared mortality rates, cerebral blood flow and extension of ischemic lesion induced by intraluminal filament in three widely used outbred rat strains. We have additionally used an animal 18F-DG PET scans to verify its reliability in noninvasive detection of ischemic infarct in acute period (24 h after MCAO) for selecting animals eligible for long survival experiments. Our data clearly indicates that high variability between rat strains might negatively influence stroke induction by intraluminal thread occlusion of middle cerebral artery. Most reliable outbred rat strain in our hands was Sprague-Dawley where maximal reduction of cerebral blood flow and extensive ischemic lesion was observed. Contrary, Wistar rats exhibited higher mortality and Long-Evans rats significantly smaller or no ischemic region in comparison to Sprague-Dawley. Additionally, we have confirmed a positron emission tomography with 18F-fluorodeoxyglucose as suitable method to assess extension of ischemic region in acute period after the experimental arterial occlusion in rats.
- MeSH
- druhová specificita MeSH
- infarkt arteria cerebri media diagnostické zobrazování genetika patofyziologie MeSH
- krysa rodu rattus MeSH
- mozkový krevní oběh fyziologie MeSH
- potkani Long-Evans MeSH
- potkani Sprague-Dawley MeSH
- potkani Wistar MeSH
- pozitronová emisní tomografie metody MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- srovnávací studie MeSH
- MeSH
- autoradiografie MeSH
- hipokampus cytologie MeSH
- krysa rodu rattus MeSH
- neurony MeSH
- vývojová biologie MeSH
- Check Tag
- krysa rodu rattus MeSH
- Publikační typ
- srovnávací studie MeSH
- MeSH
- diazepam aplikace a dávkování farmakologie MeSH
- krysa rodu rattus MeSH
- mozková kůra embryologie fyziologie účinky léků MeSH
- paměť MeSH
- těhotenství u zvířat účinky léků MeSH
- těhotenství MeSH
- učení MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- těhotenství MeSH
- ženské pohlaví MeSH
- zvířata MeSH
