The DNA damage response is mediated by both DNA repair proteins and epigenetic markers. Here, we observe that N6-methyladenosine (m6A), a mark of the epitranscriptome, was common in RNAs accumulated at UV-damaged chromatin; however, inhibitors of RNA polymerases I and II did not affect the m6A RNA level at the irradiated genomic regions. After genome injury, m6A RNAs either diffused to the damaged chromatin or appeared at the lesions enzymatically. DNA damage did not change the levels of METTL3 and METTL14 methyltransferases. In a subset of irradiated cells, only the METTL16 enzyme, responsible for m6A in non-coding RNAs as well as for splicing regulation, was recruited to microirradiated sites. Importantly, the levels of the studied splicing factors were not changed by UVA light. Overall, if the appearance of m6A RNAs at DNA lesions is regulated enzymatically, this process must be mediated via the coregulatory function of METTL-like enzymes. This event is additionally accompanied by radiation-induced depletion of 2,2,7-methylguanosine (m3G/TMG) in RNA. Moreover, UV-irradiation also decreases the global cellular level of N1-methyladenosine (m1A) in RNAs. Based on these results, we prefer a model in which m6A RNAs rapidly respond to radiation-induced stress and diffuse to the damaged sites. The level of both (m1A) RNAs and m3G/TMG in RNAs is reduced as a consequence of DNA damage, recognized by the nucleotide excision repair mechanism.

• MeSH
• Adenosine analogs & derivatives   metabolism   MeSH
• Chromatin metabolism   MeSH
• DNA Demethylation radiation effects   MeSH
• Stress, Physiological radiation effects   MeSH
• Guanosine analogs & derivatives   metabolism   MeSH
• DNA Methylation genetics   radiation effects   MeSH
• Methylation radiation effects   MeSH
• Mice MeSH
• Cell Line, Tumor MeSH
• RNA, Untranslated metabolism   MeSH
• Genomic Instability radiation effects   MeSH
• DNA Damage MeSH
• RNA metabolism   MeSH
• Ultraviolet Rays * MeSH
• Animals MeSH
• Check Tag
• Mice MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH

• MeSH
• Urinalysis methods   instrumentation   MeSH
• Blood Chemical Analysis methods   instrumentation   MeSH
• Magnesium blood   urine   MeSH
• Clinical Chemistry Tests * methods   instrumentation   MeSH
• Humans MeSH
• Reproducibility of Results MeSH
• Check Tag
• Humans MeSH

The affiliation number 1, "Institute of Biophysics of the Czech Academy of Sciences, Královopolská 135, 612 65 Brno, Czech Republic" changed its zip code to 612 00 [...].

• Publication type
• Published Erratum MeSH

• MeSH
• Cell Growth Processes MeSH
• Child MeSH
• Hormone Replacement Therapy MeSH
• Genetics, Medical MeSH
• Medical Oncology MeSH
• Human Growth Hormone deficiency   MeSH
• Neoplasms genetics   MeSH
• Growth Disorders genetics   MeSH
• Check Tag
• Child MeSH
• Publication type
• Handbook MeSH

• Conspectus
• Patologie. Klinická medicína
• NML Fields
• endokrinologie
• genetika, lékařská genetika
• onkologie

• MeSH
• Genetics, Medical MeSH
• Molecular Biology MeSH
• Growth Disorders genetics   MeSH
• Short Stature Homeobox Protein deficiency   MeSH
• Publication type
• Handbook MeSH

• Conspectus
• Patologie. Klinická medicína
• NML Fields
• endokrinologie
• genetika, lékařská genetika

• MeSH
• DNA analysis   MeSH
• Genetic Techniques MeSH
• Genetics, Medical MeSH
• Human Growth Hormone deficiency   MeSH
• Growth Disorders genetics   MeSH
• Publication type
• Handbook MeSH

• Conspectus
• Patologie. Klinická medicína
• NML Fields
• endokrinologie
• genetika, lékařská genetika

OBJECTIVES: This study evaluates the effect of post-activation potentiation (PAP) after 5x5s maximal voluntary isometric contractions (activation stimulus, AS) on tensiomyography (TMG) and torque twitch contractile parameters of vastus lateralis (VL) and medialis (VM), respectively. Further, we validated the decomposition of TMG response to separate responses of three fiber types. METHODS: 15 healthy individuals participated in this study (40% women; age 19±2.3 years). A decomposition of VL TMG response was done after optimal fitting of three exponential curves. RESULTS: We found main effects in contraction time (Tc) for muscle, method and time. Furthermore, we found interactions between muscle*method, method*time and muscle*method*time. Compared to PRE AS, we found shorter TMG Tc in VL and VM during the first two minutes after AS. Torque Tc remained unchanged in VL, while it increased in VM within 30 seconds after AS. A decomposition of VL TMG response confirmed PAP effects being present only in decomposed type IIb muscle fibers. CONCLUSION: The TMG is a sensitive method to detect PAP effects with a sensor mounted directly above the muscle belly. After the decomposition of the TMG signal to three separate muscle fiber phenotypes, we provided a non-invasive insight in the contribution of each muscle fiber phenotype to the PAP of the whole muscle.

• MeSH
• Electromyography MeSH
• Isometric Contraction * physiology   MeSH
• Muscle, Skeletal physiology   MeSH
• Humans MeSH
• Muscle Contraction * physiology   MeSH
• Torque MeSH
• Check Tag
• Humans MeSH
• Male MeSH
• Female MeSH
• Publication type
• Journal Article MeSH

• MeSH
• Hormone Replacement Therapy MeSH
• Dwarfism, Pituitary genetics   therapy   MeSH
• Clinical Studies as Topic MeSH
• Genetics, Medical MeSH
• Human Growth Hormone genetics   therapeutic use   MeSH
• Mutation genetics   MeSH
• Neuroendocrinology MeSH
• Growth and Development genetics   MeSH
• Developmental Disabilities genetics   therapy   MeSH
• Publication type
• Handbook MeSH

• Conspectus
• Obecná genetika. Obecná cytogenetika. Evoluce
• NML Fields
• pediatrie
• genetika, lékařská genetika
• endokrinologie

The diagnostic accuracy of tensiomyography (TMG) parameters compared to the gold standard in neuromuscular fatigue evaluation using voluntary and electrically induced muscle activation is unclear. This study aimed to investigate the diagnostic accuracy of TMG parameters to detect individual changes after interventions that were designed to induce central or peripheral fatigue. Nineteen males (age: 32.2 ± 9.3 years) performed two interventions, consisting of maintaining 25% of maximal voluntary contraction (MViC25%) and a 30 s all-out cycling test (Wingate), respectively. TMG parameters, maximum voluntary contraction (PtMViC), voluntary activation (VA%) and electrically elicited double twitches (Dtw) were assessed on the knee extensors before (PRE), one minute (POST) and seven minutes after (POST7) the intervention. The diagnostic accuracy (AUC) of TMG parameters were evaluated in comparison to two criteria measures (PtMViC and Dtw). RM ANOVA revealed a significant interaction between the effects of intervention and time on VA% (p = 0.001) and Dtw (p < 0.001) but not for PtMViC (p = 0.420). AUC showed that TMG parameters had a good ability in detecting muscular fatigue assessed by Dtw but not by PtMViC. The results of the current study suggest that TMG parameters can be used to monitor peripheral neuromuscular fatigue.

• MeSH
• Lower Extremity MeSH
• Adult MeSH
• Electromyography MeSH
• Knee Joint physiology   MeSH
• Knee physiology   MeSH
• Muscle, Skeletal * physiology   MeSH
• Humans MeSH
• Young Adult MeSH
• Muscle Contraction physiology   MeSH
• Muscle Fatigue * physiology   MeSH
• Check Tag
• Adult MeSH
• Humans MeSH
• Young Adult MeSH
• Male MeSH
• Publication type
• Journal Article MeSH

This systematic literature review (SLR) aimed to comprehensively synthesize existing studies that have reported on TMG-derived parameters of lower extremities in soccer players. The PubMed, Web of Science, and EBSCOHost (including MEDLINE, SPORTDiscuss, ERIC, DOAJ, and SCOPUS) databases were searched from inception to the 31st of August, 2023. Reports were eligible if they satisfied the following criteria: recruited active soccer players, with no restriction on race, sex, age, level of expertise, or health status; studies utilizing TMG for measuring muscle contractile properties. In total, 25 published journal articles from 22 original studies were included in the current review, encompassing a total of 1224 participants (4% females). The analysis considered various muscles, with the biceps femoris (BF), rectus femoris (RF), vastus lateralis (VL), vastus medialis (VM), semitendinosus (ST), gastrocnemius lateralis (GL), and gastrocnemius medialis (GM) being investigated. Significant variations were observed in TMG parameters across different muscles, age categories, and levels of play. The quality of evidence varied from low to moderate for all analyses. The meta-regression analysis indicated that age moderated several TMG-derived parameters in lower limb muscles including BF Vc, RF Td and Vc, ST Dm and sustain time, VL Dm, Tc, Td and relaxation time (Tr), and VM Tc, Td and Tr, respectively. In conclusion, the current review illuminated the multifaceted applications of TMG in assessing lower extremity muscles in soccer players. Beyond evaluating muscle contractile properties in various superficial muscles of the lower limbs in soccer players, TMG-derived parameters may serve as potentially valuable markers in identifying neuromuscular risk factors for anterior cruciate ligament injuries and predicting hamstring-related injuries.

• Publication type
• Journal Article MeSH
• Review MeSH