Wang, D Y*
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It was to investigate the clinical efficacy of the combination therapy of fluticasone propionate inhalation aerosol and vitamin D (VD) in pediatric bronchial asthma (BA) and analyze the correlation between serum 25-(OH)-D3 levels and immune function, as well as calcium-phosphorus metabolism. A total of 110 patients with BA were recruited. Regarding treatment plan, patients were randomly rolled into a single-drug treatment group (SDT, treated with fluticasone propionate inhalation aerosol alone) and a dual-drug treatment group (TDT, treated with the combination of fluticasone propionate inhalation aerosol and VD). The changes in serum 25-(OH)-D3 levels, immunoglobulins, T lymphocyte subsets, and inflammatory cytokine levels in children with BA under different treatment modalities were compared. Clinical symptom disappearance, asthma control, and quality of life (QoL) were assessed, and the total effective rate and adverse reactions (ARs) were compared. A control group consisting of 60 healthy children who underwent concurrent physical examinations was included. The differences in serum 25-(OH)-D3 levels, immunoglobulins, and T lymphocyte subset levels between children with BA and healthy controls were compared, and their correlations were analyzed. The TDT group showed a drastic reduction in the disappearance time of lung wheezing and dyspnea relative to the SDT group. Furthermore, the TDT group exhibited notable improvements in lung function parameters, including forced vital capacity (FVC), forced expiratory volume at one second (FEV1), FEV1/FVC, and peak expiratory flow (PEF). Blood gas analysis revealed a great decrease in PaCO2 and an increase in PaO2. The Childhood Asthma Control Test (C-ACT) scores for asthma control and Pediatric Asthma Quality of Life Questionnaire (PAQLQ) scores for QoL showed marked increases in the TDT group. Moreover, the TDT group demonstrated notable increases in serum 25-(OH)-D3 levels, immunoglobulins (IgA, IgG, and IgM), T lymphocyte subsets (CD4+ and CD8+), as well as blood calcium and phosphorus levels. Additionally, the TDT group exhibited a prominent increase in the anti-inflammatory cytokine interleukin (IL)-10 level and a drastic decrease in the pro-inflammatory cytokines IL-6 and tumor necrosis factor alpha (TNF-alpha) levels (all P<0.05). The total effective rates of treatment in the SDT group and TDT group were 83.64 % and 96.36 %, respectively, with AR rates of 16.36 % and 7.27 %. The TDT group exhibited a superior total effective rate and an inferior incidence of ARs to the SDT group (both P<0.05). Additionally, in contrast to the control group, the BA group showed notable decreases in serum 25-(OH)-D3 levels, immunoglobulins (IgA, IgG, and IgM), T lymphocyte subsets (CD4+, CD8+, and CD4+/CD8+), as well as blood calcium and phosphorus levels (all P<0.05). Prior to treatment, there was a positive correlation between serum 25-(OH)-D3 levels and immunoglobulins (IgA, IgG, and IgM), T lymphocyte subsets (CD4+ and CD8+), as well as blood calcium and phosphorus levels in children with BA (P<0.05). In patients with BA, combined treatment with inhaled fluticasone propionate aerosol and VD may have a regulatory effect on serum 25-hydroxyVD levels, immune function, and calcium-phosphate metabolism. The correlation between serum 25-(OH)-D3 levels and immune function, as well as calcium-phosphate metabolism, suggested that VD may play a crucial role in the immune regulation and calcium-phosphate metabolism of BA.
- MeSH
- aplikace inhalační MeSH
- bronchiální astma * farmakoterapie krev imunologie MeSH
- bronchodilatancia aplikace a dávkování terapeutické užití MeSH
- dítě MeSH
- flutikason aplikace a dávkování terapeutické užití MeSH
- fosfáty krev MeSH
- kombinovaná farmakoterapie * MeSH
- kvalita života MeSH
- lidé MeSH
- mladiství MeSH
- předškolní dítě MeSH
- vápník krev MeSH
- vitamin D * krev analogy a deriváty terapeutické užití MeSH
- výsledek terapie MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- mladiství MeSH
- mužské pohlaví MeSH
- předškolní dítě MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- randomizované kontrolované studie MeSH
Cíl Provést systematický přehled prospektivních studií hodnotících vztah mezi příjmem vitaminu D nebo koncentrací 25-hydroxyvitaminu D [25(OH)D] v krvi a rizika kolorektálního karcinomu ve formě metaanalýzy. Metody Relevantní studie byly vyhledány v databázích MEDLINE a EMBASE bez jakýchkoli omezení, a to do října 2010. Byly zařazeny prospektivní studie, které uváděly odhady relativního rizika (relative risk, RR) s 95% intervalem spolehlivosti (confi dence interval, CI) pro vztah příjmu vitaminu D nebo koncentrace 25(OH)D v krvi s rizikem kolorektálního karcinomu, karcinomu tlustého střeva nebo karcinomu rekta. Do naší analýzy bylo zahrnuto přibližně 1 000 000 účastníků z několika zemí. Výsledky Do metaanalýzy bylo zahrnuto devět studií příjmu vitaminu D a devět studií koncentrace 25(OH)D v krvi. Sdružená RR kolorektálního karcinomu vypočtená pro nejvyšší kategorii proti nejnižším kategoriím příjmu vitaminu D a pro tyto kategorie u koncentrace 25(OH)D v krvi byla 0,88 (95% CI 0,80–0,96), resp. 0,67 (95% CI 0,54–0,80). Nebyla zjištěna žádná heterogenita mezi studiemi příjmu vitaminu D (p = 0,19) ani mezi studiemi koncentrace 25(OH)D v krvi (p = 0,96). Zvýšení koncentrace 25(OH)D v krvi o 10 ng/ml bylo spojeno s hodnotou RR 0,74 (95% CI 0,63–0,89). Závěr V této metaanalýze byly příjem vitaminu D a koncentrace 25(OH)D v krvi ve vztahu nepřímé úměry s rizikem kolorektálního karcinomu.
- MeSH
- kohortové studie MeSH
- kolorektální nádory epidemiologie krev prevence a kontrola MeSH
- lidé MeSH
- prospektivní studie MeSH
- rizikové faktory MeSH
- studie případů a kontrol MeSH
- vitamin D analogy a deriváty aplikace a dávkování krev MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- práce podpořená grantem MeSH
- přehledy MeSH
- MeSH
- alopurinol * terapeutické užití MeSH
- diabetes mellitus 2. typu * farmakoterapie metabolismus patofyziologie MeSH
- dospělí MeSH
- hodnoty glomerulární filtrace fyziologie MeSH
- hyperurikemie * farmakoterapie metabolismus MeSH
- ledviny * metabolismus účinky léků MeSH
- lidé středního věku MeSH
- lidé MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- komentáře MeSH
- souhrny MeSH
Autophagy can regulate cell growth, proliferation, and stability of cell environment. Its dysfunction can be involved in a variety of diseases. Hydrogen sulfide (H(2)S) is an important signaling molecule that regulates many physiological and pathological processes. Recent studies indicate that H(2)S plays an important protective role in many diseases through influencing autophagy, but its mechanism is not fully understood. This article reviewed the progress about the effect of H(2)S on autophagy in diseases in recent years in order to provide theoretical basis for the further research on the interaction of H(2)S and autophagy and the mechanisms involved.
- MeSH
- autofagie fyziologie MeSH
- cytoprotekce účinky léků fyziologie MeSH
- gasotransmitery metabolismus MeSH
- ischemie farmakoterapie metabolismus MeSH
- lidé MeSH
- proliferace buněk účinky léků fyziologie MeSH
- sulfan aplikace a dávkování metabolismus MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- přehledy MeSH
Citrulline ureidase (CTU, EC3.5.1.20) degrades citrulline into ornithine, carbon dioxide, and ammonia. Here, we present the report on expression of recombinant CTU in Escherichia coli. The soluble and active recombinant CTU was expressed in the periplasmic space with the vector pET-22b and the His-tagged CTU was purified with Ni-Affinity Chromatography. The yield of soluble recombinant protein was significantly increased when 1% sorbitol was supplemented in medium. By using phenylisothiocyanate (PITC) pre-column derivatization HPLC, the enzyme activity of recombinant CTU was determined via measuring of the substrate citrulline and the corresponding products. Our results could be useful in the study of CTU biochemical characteristics, enzymatic preparation of ornithine and development of an enzymatic detection method of citrulline.
- MeSH
- Bacteria MeSH
- bakteriologické techniky metody využití MeSH
- chromatografie afinitní metody využití MeSH
- citrulin * izolace a purifikace metabolismus MeSH
- enzymatické testy metody využití MeSH
- Escherichia coli enzymologie imunologie izolace a purifikace MeSH
- Francisella tularensis * enzymologie izolace a purifikace metabolismus MeSH
- hydrolasy izolace a purifikace MeSH
- ornithin * izolace a purifikace metabolismus MeSH
- rekombinantní proteiny genetika izolace a purifikace metabolismus MeSH
- sorbitol diagnostické užití MeSH
- statistika jako téma MeSH
- tularemie diagnóza etiologie mikrobiologie MeSH
- ureasa izolace a purifikace metabolismus MeSH
- vysokoúčinná kapalinová chromatografie metody využití MeSH
- Publikační typ
- práce podpořená grantem MeSH
Xylanase III (Xyn III), a specific endoxylanase that belongs to family 10 of the glycoside hydrolases, was overexpressed in Trichoderma reesei QM9414 using a constitutive strong promoter of the gene encoding pyruvate decarboxylase (pdc). The maximum recombinant xylanase activity achieved was 817.2 ± 65.2 U/mL in the transformant fermentation liquid. The productivities of Xyn III accounted for approximately 53% of the total protein secreted by the recombinant. The enzyme was optimally active at 60 °C and pH 6. The recombinant Xyn III was stable at pH 5-8. This is the first report on the homologous expression of xyn3 in T. reesei QM9414. The properties of Xyn III make it promising in a variety of industrial use.
Diabetic kidney disease (DKD) is the leading cause of end-stage renal disease. Therefore, understanding the molecular regulatory mechanisms underlying the pathogenesis of DKD is imperative. In this study, we aimed to explore the molecular mechanisms of tubule region endothelial dysfunction in early DKD. Early-stage DKD model was established in 16-week-old female db/db mice for 16 weeks. Body weight, glucose level, and urine albumin-to-creatinine ratio (UACR) were measured. Hematoxylin and eosin (H&E) and periodic acid-Schiff (PAS) staining were performed to evaluate pathological lesions. RNA sequencing data of the kidneys and integrated publicly available single-cell and spatial transcriptome datasets were used to investigate the mechanism of endothelial dysfunction. There was a significant increase in body weight (p = 0.001), glucose levels (p=0.0008), and UACR (p=0.006) in db/db mice compared with db/m mice. H&E and PAS staining showed that vacuolar lesions and protein casts of tubules were the major histopathological changes observed in early-stage DKD mice. The apoptotic pathway in endothelial cells was notably activated in DKD, and Thbs1 was identified as the central gene involved in this apoptotic process. Deconvolution of the cell composition in the RNA sequencing data showed a decrease in the proportion of endothelial cells in the DKD mice. Further analysis of the activity and regulatory network of transcription factors showed that Creb1 was activated in both mouse and human early-stage DKD, suggesting that Creb1 activation may be involved in early kidney injury. The endothelial cell apoptotic pathway is activated in DKD, and the proportion of endothelial cells was reduced in the DKD mice, which is significantly associated with Thbs1. Keywords: Diabetic kidney disease, Endothelial dysfunction, RNA sequencing,Thbs1, Creb1.
- MeSH
- apoptóza MeSH
- diabetické nefropatie * patologie metabolismus patofyziologie genetika MeSH
- endoteliální buňky metabolismus patologie MeSH
- ledvinové kanálky patologie metabolismus MeSH
- myši inbrední C57BL MeSH
- myši MeSH
- progrese nemoci * MeSH
- protein vázající cAMP responzivní element metabolismus genetika MeSH
- thrombospondin 1 metabolismus genetika MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- MeSH
- dospělí MeSH
- elektronické zdravotní záznamy MeSH
- karcinom MeSH
- lidé středního věku MeSH
- lidé MeSH
- lymfatické metastázy MeSH
- lymfatické uzliny patologie MeSH
- mnohočetné primární nádory chirurgie patologie MeSH
- nádory hlavy a krku epidemiologie patologie sekundární MeSH
- nádory štítné žlázy chirurgie patologie MeSH
- nemocnice univerzitní MeSH
- papilární karcinom epidemiologie chirurgie patologie sekundární MeSH
- pohlavní dimorfismus MeSH
- průřezové studie MeSH
- retrospektivní studie MeSH
- rizikové faktory MeSH
- tumor burden MeSH
- věkové faktory MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- souhrny MeSH
- Geografické názvy
- Čína MeSH
