cervical fluid Dotaz Zobrazit nápovědu
OBJECTIVE: To determine the cervical and vaginal fluid soluble Toll-like receptor-2 (sTLR2) levels in pregnancies complicated by preterm prelabor rupture of membranes (PPROM) and their correlation to microbial invasion of the amniotic cavity (MIAC) and/or histological chorioamnionitis (HCA). METHODS: Sixty-eight women with singleton pregnancies complicated by PPROM were included in this study. Cervical and vaginal fluid was collected at the time of admission, and levels of sTLR2 in the cervical and vaginal fluid were determined using enzyme-linked immunosorbent assay. RESULT: Women with MIAC and both MIAC and HCA did not have different cervical and vaginal fluid sTLR2 levels compared to those without MIAC and without both MIAC and HCA. Women with HCA had higher cervical fluid sTLR2 levels in crude analysis (with HCA: median 11.6 pg/mL versus without HCA: median 5.5 pg/mL; p = 0.04) but not after adjustment for gestational age at sampling (p = 0.19). No difference in vaginal fluid sTLR2 levels between women with and without HCA was found. A positive correlation between cervical and vaginal fluid sTLR2 levels was identified (rho = 0.54; p < 0.0001). CONCLUSIONS: Cervical and vaginal fluid sTLR2 levels did not reflect the presence of MIAC and/or HCA.
- MeSH
- cervix uteri metabolismus MeSH
- chorioamnionitida metabolismus MeSH
- dospělí MeSH
- ELISA MeSH
- lidé MeSH
- novorozenec MeSH
- plodová voda mikrobiologie MeSH
- předčasný odtok plodové vody metabolismus patologie MeSH
- těhotenství MeSH
- tělesné tekutiny metabolismus MeSH
- toll-like receptor 2 metabolismus MeSH
- vagina metabolismus MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- novorozenec MeSH
- těhotenství MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- MeSH
- cysty diagnóza MeSH
- edém komplikace MeSH
- krční obratle zranění MeSH
- lidé MeSH
- mozkomíšní mok fyziologie MeSH
- nemoci míchy komplikace MeSH
- shunty pro odvod mozkomíšního moku MeSH
- ventrikulostomie MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- Publikační typ
- kazuistiky MeSH
UNLABELLED: Abstract Objective: To determine the cervical fluid interleukin (IL)-6 and IL-8 levels in pregnancies complicated by preterm prelabor rupture of membranes (PPROM) and the association of these interleukins with microbial invasion of the amniotic cavity (MIAC) and histological chorioamnionitis (HCA). METHODS: Sixty women with singleton pregnancies were included in this study. Cervical fluid was sampled at the time of admission using Dacron polyester swabs, which were placed into the endocervical canal for 20 s. IL-6 and IL-8 levels were determined by ELISA. The management of PPROM was active management (except for in pregnancies <28 weeks of gestation) and occurs not later than 72 h after the rupture of membranes. RESULT: The women with MIAC had higher IL-6 and IL-8 levels than did the women without MIAC (IL-6: p=0.01; IL-8: p=0.003). There was no difference in IL-6 levels between women with and without HCA (p=0.37). The women with HCA had higher IL-8 levels only in the crude analysis (p=0.01) but not after adjustment for gestational age (p=0.06). The women with both MIAC and HCA had higher levels of IL-6 and IL-8 than did the other women (IL-6: p=0.003; IL-8: p=0.001). IL-8 level of 2653 pg/mL was found to be the best cut-off point in the identification of PPROM pregnancies complicated by both MIAC and HCA with a likelihood ratio of 24. CONCLUSIONS: The presence of MIAC is the most important factor impacting the local cervical inflammatory response, which is determined by IL-6 and IL-8 levels in the cervical fluid. IL-8 levels seem to be a promising non-invasive marker for the prediction of pregnancies complicated by the presence of both MIAC and HCA.
- MeSH
- amnion metabolismus mikrobiologie patologie MeSH
- cervix uteri chemie metabolismus MeSH
- chorioamnionitida diagnóza metabolismus mikrobiologie patologie MeSH
- dospělí MeSH
- infekční komplikace v těhotenství diagnóza metabolismus MeSH
- interleukin-6 analýza metabolismus MeSH
- interleukin-8 analýza metabolismus MeSH
- lidé MeSH
- mladý dospělý MeSH
- plodová voda chemie metabolismus MeSH
- předčasný odtok plodové vody metabolismus MeSH
- těhotenství MeSH
- tělesné tekutiny chemie metabolismus MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mladý dospělý MeSH
- těhotenství MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
PROBLEM: To determine the changes of pentraxin 3 (PTX3) level in noninvasively obtained cervical fluid samples from women with preterm prelabor rupture of membranes (PPROM) based on the presence of microbial invasion of the amniotic cavity (MIAC) and/or intra-amniotic inflammation (IAI), and intra-amniotic infection (the presence of both MIAC and IAI). METHODS OF STUDY: A total of 160 women with PPROM were included. Cervical fluid samples were obtained using a Dacron polyester swab and amniotic fluid samples were obtained by transabdominal amniocentesis. Cervical fluid PTX3 levels were assessed using enzyme-linked immunosorbent assay. RESULTS: PTX3 was found in all the cervical fluid samples and its levels were higher in women with MIAC, IAI, and intra-amniotic infection than in women without these conditions. When the women were categorized into four subgroups based on the presence of MIAC and/or IAI, women with intra-amniotic infection had higher cervical fluid PTX3 levels than those with sterile IAI (IAI alone), colonization (MIAC alone), or no MIAC or IAI. A cervical fluid PTX3 level of 11 ng/mL was the best value for identifying the presence of intra-amniotic infection in women with PPROM. CONCLUSIONS: PTX3 is a constituent of cervical fluid of women with PPROM. Cervical fluid PTX3 level reflects the situation in the intra-amniotic compartments of women with PPROM. Cervical fluid PTX3 is a potential marker for the noninvasive identification of intra-amniotic infection in PPROM.
- MeSH
- biologické markery metabolismus MeSH
- C-reaktivní protein metabolismus MeSH
- cervix uteri metabolismus MeSH
- chorioamnionitida diagnóza metabolismus mikrobiologie MeSH
- ELISA MeSH
- lidé MeSH
- plodová voda mikrobiologie MeSH
- předčasný odtok plodové vody diagnóza metabolismus mikrobiologie MeSH
- prediktivní hodnota testů MeSH
- retrospektivní studie MeSH
- sérový amyloidový protein metabolismus MeSH
- těhotenství MeSH
- upregulace MeSH
- Check Tag
- lidé MeSH
- těhotenství MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
OBJECTIVE: To evaluate Ureaplasma species and Mycoplasma hominis DNA in the cervical fluid and their association with microbial invasion of the amniotic cavity (MIAC) and/or histological chorioamnionitis (HCA) in pregnancies complicated by preterm prelabor rupture of membranes (PPROM). STUDY DESIGN: A prospective study of 68 women with singleton pregnancies complicated by PPROM between 24(0/7) and 36(6/7) weeks was conducted. Cervical fluid and amniotic fluid were collected from all women at the time of admission. The Ureaplasma species and Mycoplasma hominis DNA in the cervical fluid were identified using specific real-time PCR. RESULTS: Ureaplasma species and Mycoplasma hominis DNA were identified in 59% (40/69) of the cervical fluid samples. Women with the presence of Ureaplasma species DNA with and without Mycoplasma hominis DNA in the cervical fluid had a higher rate of MIAC alone [35% (14/40) versus 11% (3/28); p = 0.02] and a higher rate of the presence of both MIAC and HCA [30% (12/40) versus 4% (1/28); p = 0.01] than women without Ureaplasma species and Mycoplasma hominis DNA in the cervical fluid. CONCLUSIONS: The presence of Ureaplasma species DNA with and without Mycoplasma hominis DNA in the cervical fluid is associated with a higher risk of MIAC or MIAC and HCA together in pregnancies complicated by PPROM.
- MeSH
- chorioamnionitida mikrobiologie MeSH
- dospělí MeSH
- lidé MeSH
- mladý dospělý MeSH
- Mycoplasma hominis izolace a purifikace MeSH
- plodová voda mikrobiologie MeSH
- předčasný odtok plodové vody mikrobiologie MeSH
- těhotenství MeSH
- Ureaplasma izolace a purifikace MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mladý dospělý MeSH
- těhotenství MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
OBJECTIVE: The study aimed to determine the cervical calreticulin and cathepsin-G concentrations in pregnancies complicated by preterm prelabor rupture of membranes (PPROM) with respect to the presence of microbial invasion of the amniotic cavity (MIAC) and intra-amniotic inflammation (IAI). METHODS: Eighty women with singleton pregnancies complicated by PPROM were included in this study. Cervical and amniotic fluids were obtained at the time of admission, and concentrations of calreticulin and cathepsin-G in cervical fluid were determined using ELISA. The MIAC was defined as a positive PCR analysis for Ureaplasma species, Mycoplasma hominis, and/or Chlamydia trachomatis and/or by positivity for the 16S rRNA gene. IAI was defined as amniotic fluid bedside IL-6 concentrations ≥745 pg/mL Result: Neither women with MIAC nor with IAI had different cervical fluid concentrations of calreticulin (with MIAC: median 18.9 pg/mL vs. without MIAC: median 14.7 pg/mL, p = 0.28; with IAI: median 14.3 pg/mL vs. without IAI: median 15.6 pg/mL, p = 0.57;) or of cathepsin-G (with MIAC: median 30.7 pg/mL vs. without MIAC: median 24.7 pg/mL, p = 0.28; with IAI: median 27.3 pg/mL vs. without IAI: median 25.1 pg/mL, p = 0.80) than women without those complications. No associations between amniotic fluid IL-6 concentrations, gestational age at sampling, and cervical fluid calreticulin and cathepsin-G concentrations were found. CONCLUSIONS: Cervical fluid calreticulin and cathepsin-G concentrations did not reflect the presence of MIAC or IAI in women with PPROM.
- MeSH
- biologické markery analýza MeSH
- chorioamnionitida diagnóza mikrobiologie MeSH
- dospělí MeSH
- ELISA MeSH
- gestační stáří MeSH
- interleukin-6 analýza MeSH
- kalretikulin analýza MeSH
- kathepsin G analýza MeSH
- lidé MeSH
- mladý dospělý MeSH
- plodová voda chemie metabolismus mikrobiologie MeSH
- předčasný odtok plodové vody metabolismus mikrobiologie MeSH
- prospektivní studie MeSH
- senzitivita a specificita MeSH
- těhotenství MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mladý dospělý MeSH
- těhotenství MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
OBJECTIVE: To determine if cervical fluid interleukin (IL)-6 concentrations in women with preterm prelabor rupture of membranes (PPROM) allows identification of microbial invasion of the amniotic cavity (MIAC) and/or intra-amniotic inflammation (IAI). METHODS: One hundred forty-four women with singleton pregnancies complicated by PPROM were included in this prospective cohort study. Cervical and amniotic fluids were collected at the time of admission and concentrations of IL-6 were measured using an ELISA and point-of-care test, respectively. Cervical fluid was obtained using a Dacron polyester swab and amniotic fluid was obtained by transabdominal amniocentesis. MIAC was diagnosed based on a positive PCR result for Ureaplasma species, M. hominis, and/or C. trachomatis and/or by positivity for the 16 S rRNA gene. IAI was defined as amniotic fluid point-of-care IL-6 concentrations ≥745 pg/mL. The women were assigned to four subgroups based on the presence of MIAC and/or IAI: microbial-associated IAI (both MIAC and IAI), sterile IAI (IAI alone), MIAC alone, and without either MIAC or IAI. RESULTS: (1) Women with microbial-associated IAI had higher cervical fluid IL-6 concentrations (median 560 pg/mL) than did women with sterile IAI (median 303 pg/mL; p = .001), women with MIAC alone (median 135 pg/mL; p = .0004), and women without MIAC and IAI (median 180 pg/mL; p = .0001). (2) No differences were found in cervical fluid IL-6 concentrations among women with sterile IAI, with MIAC alone, and without MIAC and IAI. (3) A positive correlation was observed between cervical fluid IL-6 concentrations and the amount of Ureaplasma species in amniotic fluid (copies DNA/mL; rho = 0.57, p < .0001). (4) A weak positive correlation was detected between cervical and amniotic fluid IL-6 concentrations (rho = 0.33, p < .0001). CONCLUSIONS: The presence of microbial-associated IAI is associated with the highest cervical fluid IL-6 concentrations. Cervical IL-6 can be helpful in the identification of microbial-associated IAI.
- MeSH
- amniocentéza MeSH
- amnion mikrobiologie MeSH
- biologické markery analýza MeSH
- chorioamnionitida diagnóza mikrobiologie MeSH
- dospělí MeSH
- ELISA MeSH
- gestační stáří MeSH
- interleukin-6 analýza MeSH
- lidé MeSH
- mladý dospělý MeSH
- novorozenec MeSH
- plodová voda chemie mikrobiologie MeSH
- point of care testing MeSH
- polymerázová řetězová reakce MeSH
- předčasný odtok plodové vody etiologie genetika metabolismus mikrobiologie MeSH
- prospektivní studie MeSH
- těhotenství MeSH
- Ureaplasma izolace a purifikace MeSH
- ureaplasmatické infekce diagnóza mikrobiologie MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mladý dospělý MeSH
- novorozenec MeSH
- těhotenství MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
Cíl studie: Zjistit,zda se fruktóza a glukóza vyskytují ve folikulární tekutině stimulovaných ženv programu IVF a zda jejich hladiny souvisí s úspěšností cyklu,se stimulačním protokolem nebos faktorem neplodnosti těchto žen.Typ studie:Retrospektivní studie.Název a sídlo pracoviště:Gynekologicko-porodnická klinika FN v Plzni.Metodika:U 202 pacientek podstupujících IVF léčbu v období 11/1999 až 02/2001 byl odebrán přiaspiraci folikulů vzorek krve a vzorek folikulární tekutiny (FF).V séru a supernatantu FF jsmeu těchto pacientek změřili hladiny fruktózy i glukózy.Výsledky jsme porovnávali s úspěšností vesledovaném cyklu,s délkou stimulačního protokolu a s faktorem neplodnosti těchto žen.Kontrolní skupinu tvořilo 23 prakticky zdravých žen,jejichž faktor neplodnosti byl jen čistě andrologický.Výsledky:U stimulovaných pacientek je v den odběru oocytů nalačno koncentrace glukózy v séruv průměru 5,43 ±1,35 mmol/l a fruktózy 25,05 ±9,26 m ml,oproti 5,26 ±1,00 mmol/l a 23,69 ±8,68m ml v kontrolní skupině.Ve FF se nachází koncentrace glukózy i fruktózy v přímé závislosti najejich sérové hladině.Průměrně 3,75 ±1,32 mmol/l glukózy;asi 70 %sérové koncentrace a 11,34 ±5,29 m ml;asi 48 %sérové hladiny.U kontrolní skupiny 3,78 ±0,91 mmol/l (73 %sérové koncentrace)glukózy a 12,37 ±7,06 m ml (53 %sérové koncentrace)fruktózy ve FF.Závěr:Předpokládáme možnost ovlivnění fertilizačního procesu jednoduchými sacharidy,jakýmijsou glukóza a fruktóza,na základě již obecně známých poznatků o fruktóze v seminální tekutině,jejím vlivu na fertilizační schopnost spermií a na základě dalších prací,které se věnovaly studiusacharidů v endometriálním sekretu a cervikálním hlenu.Předpokládali jsme,že u pacienteks úspěšným cyklem bude pravděpodobně koncentrace fruktózy ve FF spíše nižší.Z našich výsledků je obsah fruktózy v séru i FF u žen,které dosáhly klinické gravidity ve sledovaném cyklu,opravdu lehce nižší,ale tento rozdíl nedosáhl v našem souboru statistické významnosti.Potvrdilijsme tedy výskyt fruktózy ani glukózy ve FF a přímou souvislost s její sérovou hladinou.Závislostkoncentrace fruktózy ve FF na úspěšnost IVF cyklu jsme však nepotvrdili.
Objective: To find out if there is some amount of fructose in the follicular fluid of IVF stimulatedpatients.I so then to compare fructose and glucose levels with IVF outcome,type of stimulationand infertility factors.Design:Retrospective study.Setting:Department of Gynaecology and Obstetrics,Charles University,the Czech Republic.Methods:202 women undergoing IVF treatment since 11/1999 till 02/2001 were evaluated.Wemeasured a level of fructose and glucose in a sample of serum and follicular fluid (FF)obtained atovum pick-up.Then the results were compared with the IVF outcome,the length of stimulationand infertility factors.The control group consists of 23 women with a pure andrologic factor ofinfertility.Results:At the day of pick-up the serum concentration of glucose and fructose taken on an emptystomach is on average 5.43 ±1.35 mmol/l and 25.05 ±9.26 m ml compared to 5.26 ±1.00 mmol/l and23.69 ±m ml in control group.The concentration of both glucose and fructose in FF correlateswith their serum level.The FF concentration of glucose is on average 3.75 ±1.32 mmol/l (70%oserum concentration)and fructose 11.34 ±5.29 m ml (48%of serum concentration)by comparisonwith 3.78 ± 0.91 mmol//l (73 %)and 12.37 ±7.06 m ml (53%)in control group.Conclusion:There is a possible influence of follicular fluid saccharides like glucose and fructosein process of fertilization.We derived this hypothesis from studies evaluating the role of sacchari-des in endometrial secretion,cervical mucous and seminal plasma.We assumed that patients witha successful IVF cycle have a lower fructose concentration in FF.The clinical pregnancy is reallymildly lower but this difference is not statistically significant.We confirmed FF fructose is inclose correlation with its serum concentration.But we did not confirm the FF glucose and FFfructose concentration is related to the IVF outcome.
OBJECTIVE: To determine the association between microbial invasion of the amniotic cavity (MIAC) and/or intra-amniotic inflammation (IAI) and the cervical prevalence of Gardnerella vaginalis DNA in pregnancies with preterm prelabor rupture of membrane (PPROM). METHOD: In total, 405 women with singleton pregnancies complicated with PPROM were included. Cervical fluid and amniotic fluid samples were collected at the time of admission. Bacterial and G. vaginalis DNA were assessed in the cervical fluid samples using quantitative PCR technique. Concentrations of interleukin-6 and MIAC were evaluated in the amniotic fluid samples. Loads of G. vaginalis DNA ≥ 1% of the total cervical bacterial DNA were used to define the cervical prevalence of G. vaginalis as abundant. Based on the MIAC and IAI, women were categorized into four groups: with intra-amniotic infection (both MIAC and IAI), with sterile IAI (IAI without MIAC), with MIAC without IAI, and without either MIAC or IAI. RESULTS: The presence of the abundant cervical G. vaginalis was related to MIAC (with: 65% vs. without: 44%; p = 0.0004) but not IAI (with: 52% vs. without: 48%; p = 0.70). Women with MIAC without IAI had the highest load of the cervical G. vaginalis DNA (median 2.0 × 104 copies DNA/mL) and the highest presence of abundant cervical G. vaginalis (73%). CONCLUSIONS: In women with PPROM, the presence of cervical G. vaginalis was associated with MIAC, mainly without the concurrent presence of IAI.
- MeSH
- cervix uteri mikrobiologie MeSH
- chorioamnionitida mikrobiologie MeSH
- dospělí MeSH
- Gardnerella vaginalis izolace a purifikace MeSH
- interleukin-6 analýza MeSH
- lidé MeSH
- plodová voda chemie mikrobiologie MeSH
- předčasný odtok plodové vody mikrobiologie MeSH
- prospektivní studie MeSH
- těhotenství MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- těhotenství MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
