complex modification
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... protein kinases -- Sulfuryl transfers: action of protein sulfotransferases and aryl sulfatases -- Modifications ... ... Protein methylation -- Protein N-acetylation -- Protein lipidation -- Proteolytic posttranslational modification ...
xxi, 490 s. : il., tab. ; 27 cm
- MeSH
- posttranslační úpravy proteinů MeSH
- proteiny metabolismus MeSH
- Publikační typ
- monografie MeSH
- Konspekt
- Biochemie. Molekulární biologie. Biofyzika
- NLK Obory
- biologie
- biochemie
- MeSH
- DNA restrikčně-modifikační enzymy izolace a purifikace klasifikace MeSH
- finanční podpora výzkumu jako téma MeSH
- hospodářská zvířata mikrobiologie MeSH
- plazmidy diagnostické užití izolace a purifikace MeSH
- restrikční enzymy izolace a purifikace klasifikace MeSH
- Staphylococcus aureus enzymologie MeSH
- Streptococcus agalactiae enzymologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Klíčová slova
- BBR 3464,
- MeSH
- adukty DNA MeSH
- DNA nádorová MeSH
- DNA MeSH
- protinádorové látky škodlivé účinky MeSH
- replikace DNA MeSH
- sloučeniny platiny metabolismus MeSH
- techniky in vitro MeSH
- vztahy mezi strukturou a aktivitou MeSH
- Publikační typ
- kongresy MeSH
Antagonisté NMDA receptorů mají silný antikonvulzivní účinek, ale také výrazné nežádoucí vedlejší účinky. Kombinace podprahových dávek nekompetitivního antagonisty NMDA receptorů dizocilpinu s antagonistou I. typu metabotropních glutamátových receptorů MPEP zesilovala antikonvulzivní působení u mláďat laboratorního potkana.
Antagonists of NMDA receptors exhibit potent anticonvulsant action but also marked unwanted side effects. Combination of a subthreshold dose of noncompetitive NMDA antagonist dizocilpine with an antagonist of glutamate metabotropic receptors MPEP in immature rats resulted in potentiation of the anticonvulsant action.
Phosphorylation of Type I restriction-modification (R-M) enzymes EcoKI, EcoAI, and EcoR124I - representatives of IA, IB, and IC families, respectively - was analysed in vivo by immunoblotting of endogenous phosphoproteins isolated from Escherichia coli strains harbouring the corresponding hsd genes, and in vitro by a phosphorylation assay using protein kinase present in subcellular fractions of E. coli. From all three R-M enzymes, the HsdR subunit of EcoKI system was the only subunit that was phosphorylated. Further, evidence is presented that HsdR is phosphorylated in vivo only when coproduced with HsdM and HsdS subunits - as part of assembled EcoKI restriction endonuclease, while the individually produced HsdR subunit is not phosphorylated. In vitro phosphorylation of the HsdR subunit of purified EcoKI endonuclease occurs on Thr, and is strictly dependent on the addition of a catalytic amount of cytoplasmic fraction isolated from E. coli. So far this is the first case of phosphorylation of a Type I R-M enzyme reported.
- MeSH
- chromatografie na tenké vrstvě MeSH
- elektroforéza MeSH
- Escherichia coli enzymologie metabolismus MeSH
- financování organizované MeSH
- fosfoaminokyseliny metabolismus MeSH
- fosforylace MeSH
- imunoprecipitace MeSH
- podjednotky proteinů metabolismus MeSH
- proteiny z Escherichia coli metabolismus MeSH
- restrikční endonukleasy typu I metabolismus MeSH
- restrikční enzymy metabolismus MeSH
- western blotting MeSH
A disturbing interaction of PAN membranes and the bradykinin generation system particularly in the presence of angiotensin converting enzyme inhibitors has been described. A modified new membrane, SPAN (special PAN), was produced by varying the polymer components in type and composition, in particular by a reduction in Na-Methallylsulfonate. Although the SPAN membrane successfully averted the bradykinin generating ability of PAN, it was important to determine whether such a modification did not lead to a loss of the satisfactory biocompatibility profile characteristic of the parent membrane. For this purpose, we conducted the present clinical study in nine patients comparing 3 membranes; (i) a polysulphone membrane (F60S); (ii) PAN; and (iii) SPAN, to examine the clinical biocompatibility profile and performance of the new membrane. A small increase in C5a with F60S and SPAN was found which is in the range expected for highly biocompatible synthetic membranes. The three dialysers had a similar inert profile for terminal complement complex arterial values, and had similar venous values. A minimal nonsignificant decline in white cell count was observed at 15 min for all dialysers, but otherwise WBC counts were unchanged. Platelet counts were unchanged throughout treatment for the three dialysers. Arterial and venous thrombin-anti-thrombin complex values were similar for all three dialysers. F60S and SPAN dialysers had similar urea clearances.(ABSTRACT TRUNCATED AT 250 WORDS)
- MeSH
- beta-2-mikroglobulin metabolismus MeSH
- biokompatibilní materiály chemie terapeutické užití MeSH
- chronické selhání ledvin metabolismus terapie MeSH
- dialýza ledvin * přístrojové vybavení MeSH
- dospělí MeSH
- komplement C5a metabolismus MeSH
- lidé středního věku MeSH
- lidé MeSH
- membranolytický komplex metabolismus MeSH
- membrány umělé * MeSH
- mladiství MeSH
- močovina metabolismus MeSH
- počet leukocytů MeSH
- senioři MeSH
- trombin metabolismus MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- senioři MeSH
- Publikační typ
- klinické zkoušky MeSH
- randomizované kontrolované studie MeSH
- MeSH
- azbest škodlivé účinky MeSH
- experimenty na zvířatech MeSH
- instilace léků MeSH
- křemen škodlivé účinky MeSH
- měď terapeutické užití MeSH
- modely nemocí na zvířatech MeSH
- plicní fibróza MeSH
- potkani Wistar MeSH
- rutin terapeutické užití MeSH
- suspenze MeSH
- trachea MeSH
- zvířata MeSH
- Check Tag
- ženské pohlaví MeSH
- zvířata MeSH
