Recombinant inbred (RI) strains (Prague HXB/BXH set) represent a unique model that allows for permanent summation of genetic and physiological information as well as the study of age-dependent changes in phenotypes and/or gene regulation. This study compared blood pressure (BP) measured in adult animals of RI strains by radiotelemetry with BP values obtained in conscious rats of comparable age subjected to short-term carotid catheterization or with those obtained by direct carotid puncture under ether anesthesia (almost 20 years ago). After radiotelemetry recording, the contribution of major vasoactive systems to BP maintenance was studied by consecutive inhibition of the renin-angiotensin system (RAS), sympathetic nervous system (SNS), and nitric oxide synthase. We found highly significant interrelationships among baseline BP values obtained by radiotelemetry, carotid catheterization, or carotid puncture. This indicates considerable stability of RI strains over the course of their long existence, and confirms the reliability of BP values used for genetic studies performed in the past. Subsequent analysis of vasoactive system participation revealed the importance of SNS for the maintenance of BP, as determined by either radiotelemetry or catheterization. The BP of catheterized rats also correlated closely with acute captopril-induced BP changes, but this was not the case for rats measured by radiotelemetry. NO-dependent vasodilatation matched the BP effects of SNS and RAS in both measuring conditions. Residual BP (recorded at sodium nitroprusside-induced dilatation of resistance vessels) was also responsible for a significant portion of the BP variation in RI strains. Our study confirms the validity of RI strains for the further genetic and physiological research of hypertension.
- MeSH
- Antihypertensive Agents pharmacology MeSH
- Financing, Organized MeSH
- Hypertension drug therapy genetics physiopathology MeSH
- Rats, Inbred Strains genetics MeSH
- Catheterization MeSH
- Blood Pressure genetics MeSH
- Rats MeSH
- Disease Models, Animal MeSH
- Monitoring, Physiologic MeSH
- Rats, Inbred SHR genetics MeSH
- Recombinant Proteins genetics MeSH
- Renin-Angiotensin System physiology MeSH
- Heart Rate genetics MeSH
- Sympathetic Nervous System physiology MeSH
- Nitric Oxide Synthase metabolism MeSH
- Telemetry MeSH
- Organ Size genetics MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Animals MeSH
- MeSH
- Hypertension MeSH
- Animals, Inbred Strains * genetics MeSH
- Recombination, Genetic MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Letter MeSH
- Comment MeSH
3rd ed. XI, 855-1807 s. : obr., tab., grafy ; 28 cm
- MeSH
- Mice, Inbred Strains MeSH
- Mice, Mutant Strains MeSH
- Mice, Transgenic MeSH
- Mice MeSH
- Check Tag
- Mice MeSH
- Conspectus
- Patologie. Klinická medicína
- NML Fields
- embryologie a teratologie
- genetika, lékařská genetika
- MeSH
- Animals, Inbred Strains MeSH
- Models, Genetic MeSH
- Disease Models, Animal MeSH
- Recombination, Genetic MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Comparative Study MeSH
- MeSH
- Alleles genetics MeSH
- Phenotype genetics MeSH
- Genetic Markers MeSH
- Hypertension genetics MeSH
- Rats, Inbred Strains genetics classification MeSH
- Rats MeSH
- Chromosome Mapping MeSH
- Recombination, Genetic MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Animals MeSH
- Publication type
- Review MeSH
- MeSH
- Hypertension genetics MeSH
- Mice, Inbred Strains MeSH
- Recombination, Genetic MeSH
- Research MeSH
- Publication type
- Review MeSH
- MeSH
- Abnormalities, Drug-Induced MeSH
- Genotype MeSH
- Rats, Inbred Strains MeSH
- Rats MeSH
- Fetus abnormalities drug effects MeSH
- Polydactyly etiology MeSH
- Teratogens MeSH
- Tretinoin adverse effects MeSH
- Fetal Development MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Animals MeSH
- Publication type
- Comparative Study MeSH
