- MeSH
- Audiovisual Aids * utilization MeSH
- Histology * education MeSH
- Microscopy MeSH
- Computer-Assisted Instruction methods trends MeSH
- Computing Methodologies MeSH
- Programmed Instructions as Topic * MeSH
- Education, Medical methods MeSH
- Publication type
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Czech Republic MeSH
With the development of light microscopy, it is becoming increasingly easy to obtain detailed multicolor fluorescence volumetric data. The need for their appropriate visualization has become an integral part of fluorescence imaging. Virtual reality (VR) technology provides a new way of visualizing multidimensional image data or models so that the entire 3D structure can be intuitively observed, together with different object features or details on or within the object. With the need for imaging advanced volumetric data, demands for the control of virtual object properties are increasing; this happens especially for multicolor objects obtained by fluorescent microscopy. Existing solutions with universal VR controllers or software-based controllers with the need to define sufficient space for the user to manipulate data in VR are not usable in many practical applications. Therefore, we developed a custom gesture-based VR control system with a custom controller connected to the FluoRender visualization environment. A multitouch sensor disk was used for this purpose. Our control system may be a good choice for easier and more comfortable manipulation of virtual objects and their properties, especially using confocal microscopy, which is the most widely used technique for acquiring volumetric fluorescence data so far.
- MeSH
- Gestures * MeSH
- Microscopy, Confocal MeSH
- Software MeSH
- Virtual Reality * MeSH
- Publication type
- Journal Article MeSH
Mezi hlavni přednosti konfokální mikroskopie ve srovnaní s klasickou optickou mikroskopii patři možnost zaznamenávat série digitalizovaných obrazů relativně tenkých optických řezů vedených i tlustším vzorkem. V článkuje ukázáno využití těchto sérií obrazů, jak při vizualizaci, tak i při kvantifikaci trojrozměrných objektů: Jsou popsány různé typy rekonstrukcí objektů, které lze proostřit konfokálním mikroskopem, i měření jejich geometrických charakteristik s využitím stereologických metod. Podrobněji je přiblížena stereologická metoda užívající virtuální fakirské sondy pro odhad objemu i povrchu studovaného objektu.
The possibility to record series of digitized images of relatively thin optical sections within a thick speciment is one of the main advantages ofconfocal mircoscopy if compared with the conventional optical microscopy. It is shown how such series can be exploited in visualization and quantification of three-dimensional objects: different types of reconstructions of objects that can be focussed through by a confocal microscope as well as the estimation of their geometrical characteristics by stereological methods are described. The stereological method using virtual fakir probes for volume and surface area estimation is presented in detail.
- MeSH
- Microscopy, Confocal MeSH
- Humans MeSH
- Software MeSH
- Computational Biology MeSH
- Check Tag
- Humans MeSH
[1st ed.] XVII, 857 s.
INTRODUCTION: Virtual microscopy, used as a method to teach histology, has many undeniable advantages. However, the usefulness of this method is somewhat limited by the difficulties students face in finding their way through huge amounts of digital data, compounded by decreased interaction between students and teachers. We describe the results of a recent pilot project which combined the modern teaching methods of active learning, where students themselves present histological slides and make use of the virtual microscopy system. METHODS: Students' responses to a structured questionnaire and examination results were evaluated. RESULTS: We found that a combination of both electronic materials and textbooks was commonly used by students to prepare for practical teaching sessions, with electronic resources being used regularly by the majority of students. No statistically relevant differences were found between the approaches of dentistry vs general medicine students. Cooperation between students' groups during the preparation for individual presentations was seen to be beneficial by a majority of dentistry students; they reported that the introduction of student-led presentations improved their quality of preparation for practical lessons, as well as increasing their participation and activity level in the lessons themselves. These different approaches and motivations between students of dentistry and general medicine are reflected in the test results where dentistry students are more successful. CONCLUSION: We confirm that there are differences in motivation, approaches and examination results between both groups of students, which should be taken into account and which could lead to differentiation of future curricula for both study courses.
- MeSH
- Histology education MeSH
- Humans MeSH
- Microscopy methods MeSH
- Pilot Projects MeSH
- Surveys and Questionnaires MeSH
- Students, Medical psychology MeSH
- Students, Dental psychology MeSH
- Education, Dental methods MeSH
- Teaching Materials MeSH
- Learning * MeSH
- Virtual Reality * MeSH
- Teaching * MeSH
- Check Tag
- Humans MeSH
- Male MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Comparative Study MeSH
BACKGROUND: Multifunctional two-photon laser scanning microscopy provides attractive advantages over conventional two-photon laser scanning microscopy. For the first time, simultaneous measurement of the second harmonic generation (SHG) signals in the forward and backward directions and two photon excitation fluorescence were achieved from the deep shade plant Selaginella erythropus. RESULTS: These measurements show that the S. erythropus leaves produce high SHG signals in both directions and the SHG signals strongly depend on the laser's status of polarization and the orientation of the dipole moment in the molecules that interact with the laser light. The novelty of this work is (1) uncovering the unusual structure of S. erythropus leaves, including diverse chloroplasts, various cell types and micromophology, which are consistent with observations from general electron microscopy; and (2) using the multifunctional two-photon laser scanning microscopy by combining three platforms of laser scanning microscopy, fluorescence microscopy, harmonic generation microscopy and polarizing microscopy for detecting the SHG signals in the forward and backward directions, as well as two photon excitation fluorescence. CONCLUSIONS: With the multifunctional two-photon laser scanning microscopy, one can use noninvasive SHG imaging to reveal the true architecture of the sample, without photodamage or photobleaching, by utilizing the fact that the SHG is known to leave no energy deposition on the interacting matter because of the SHG virtual energy conservation characteristic.
- MeSH
- Chloroplasts chemistry ultrastructure MeSH
- Microscopy, Confocal methods MeSH
- Plant Leaves chemistry ultrastructure MeSH
- Microscopy, Fluorescence, Multiphoton methods MeSH
- Image Processing, Computer-Assisted methods MeSH
- Selaginellaceae chemistry ultrastructure MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
