PIN proteins perform a rate-limiting function in cellular auxin efflux
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.
PubMed
16601150
DOI
10.1126/science.1123542
PII: 1123542
Knihovny.cz E-resources
- MeSH
- ATP-Binding Cassette Transporters genetics metabolism MeSH
- Arabidopsis cytology growth & development metabolism physiology MeSH
- Biological Transport MeSH
- Cell Membrane metabolism MeSH
- Phthalimides pharmacology MeSH
- Gravitropism MeSH
- HeLa Cells MeSH
- Kinetics MeSH
- Plant Roots physiology MeSH
- Cells, Cultured MeSH
- Indoleacetic Acids metabolism MeSH
- Naphthaleneacetic Acids metabolism MeSH
- Humans MeSH
- Membrane Transport Proteins genetics metabolism MeSH
- Mutation MeSH
- Arabidopsis Proteins genetics metabolism MeSH
- Saccharomyces cerevisiae genetics MeSH
- Nicotiana MeSH
- Transfection MeSH
- Transformation, Genetic MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
- Names of Substances
- 1-naphthaleneacetic acid MeSH Browser
- ATP-Binding Cassette Transporters MeSH
- alpha-naphthylphthalamic acid MeSH Browser
- ATPGP1 protein, Arabidopsis MeSH Browser
- Phthalimides MeSH
- Indoleacetic Acids MeSH
- Naphthaleneacetic Acids MeSH
- Membrane Transport Proteins MeSH
- PIN1 protein, Arabidopsis MeSH Browser
- PIN2 protein, Arabidopsis MeSH Browser
- PIN4 protein, Arabidopsis MeSH Browser
- PIN7 protein, Arabidopsis MeSH Browser
- Arabidopsis Proteins MeSH
Intercellular flow of the phytohormone auxin underpins multiple developmental processes in plants. Plant-specific pin-formed (PIN) proteins and several phosphoglycoprotein (PGP) transporters are crucial factors in auxin transport-related development, yet the molecular function of PINs remains unknown. Here, we show that PINs mediate auxin efflux from mammalian and yeast cells without needing additional plant-specific factors. Conditional gain-of-function alleles and quantitative measurements of auxin accumulation in Arabidopsis and tobacco cultured cells revealed that the action of PINs in auxin efflux is distinct from PGP, rate-limiting, specific to auxins, and sensitive to auxin transport inhibitors. This suggests a direct involvement of PINs in catalyzing cellular auxin efflux.
References provided by Crossref.org
WAVY GROWTH Arabidopsis E3 ubiquitin ligases affect apical PIN sorting decisions
Lessons Learned from the Studies of Roots Shaded from Direct Root Illumination
GmPIN-dependent polar auxin transport is involved in soybean nodule development
DIOXYGENASE FOR AUXIN OXIDATION 1 catalyzes the oxidation of IAA amino acid conjugates
No Time for Transcription-Rapid Auxin Responses in Plants
AGC kinases and MAB4/MEL proteins maintain PIN polarity by limiting lateral diffusion in plant cells
The TOR-Auxin Connection Upstream of Root Hair Growth
The lipid code-dependent phosphoswitch PDK1-D6PK activates PIN-mediated auxin efflux in Arabidopsis
Salicylic Acid Targets Protein Phosphatase 2A to Attenuate Growth in Plants
CRK5 Protein Kinase Contributes to the Progression of Embryogenesis of Arabidopsis thaliana
The Nuts and Bolts of PIN Auxin Efflux Carriers
Auxins and Cytokinins-The Role of Subcellular Organization on Homeostasis
WRKY23 is a component of the transcriptional network mediating auxin feedback on PIN polarity
What Has Been Seen Cannot Be Unseen-Detecting Auxin In Vivo
