- MeSH
- elektronické předepisování * ekonomika MeSH
- monitorování léčiv * metody MeSH
- Publikační typ
- novinové články MeSH
- rozhovory MeSH
A novel procedure for the determination of stability constants in systems with neutral analytes and charged complexation agents by affinity capillary electrophoresis was established. This procedure involves all necessary corrections to achieve precise and reliable data. Temperature, ionic strength, and viscosity corrections were applied. Based on the conductivity measurements, the average temperature of the background electrolyte in the capillary was kept at the constant value of 25°C by decreasing the temperature of the cooling medium. The viscosity correction was performed using the viscosity ratio determined by an external viscosimeter. The electrophoretical measurements were performed, at first, at constant ionic strength. In this case, the increase of ionic strength caused by increasing complexation agent concentration was compensated by changing of the running buffer concentration. Subsequently the dependence of the analyte effective mobility on the complexation agent concentration was measured without the ionic strength compensation (at variable ionic strength). The new procedure for determination of the stability constants even from such data was established. These stability constants are in a very good agreement with those obtained at the constant ionic strength. The established procedure was applied for determination of the thermodynamic stability constants of (R, R)-(+)- and (S, S)-(-)-hydrobenzoin and R- and S-(3-bromo-2-methylpropan-1-ol) complexing with 6-monodeoxy-6-mono(3-hydroxy)propylamino-β-cyclodextrin hydrochloride.
The complete mathematical model of electromigration dispersion in systems that contain a neutral complex forming agent and a fully charged analyte was introduced in the previous part of this series of papers (Part III - Theory). The model was implemented in the newest version of our simulation program PeakMaster 5.3 that calculates the effective mobility of the analyte and its nonlinear electromigration mobility slope, S(EMD), in the presence of a complex forming agent in the background electrolyte. The mathematical model was verified by both experiments and simulations, which were performed by our dynamic simulator Simul 5 Complex. Three separation systems differing in the chiral selector used (having different values for the complexation constant and the mobility of the complex) were chosen for the verification. The nonlinear electromigration mobility slope values were calculated from the simulations and the experiments that were performed at different complex forming agent concentrations. These data agree very well with those predicted by the mathematical model and provided the foundation for the discussion and explanation of the electromigration dispersion process that occurs in systems which contain a complex forming agent. The new version of PeakMaster 5.3 was shown to be a powerful tool for optimization of the separation conditions by minimizing electromigration dispersion which improves the symmetry of the analyte peaks and their resolution.
- MeSH
- farmaceutický průmysl MeSH
- generika terapeutické užití MeSH
- lidé MeSH
- náhrada léků využití MeSH
- spotřeba léčiv statistika a číselné údaje MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- novinové články MeSH
Centrální úložiště elektronických receptů bylo zřízeno dle zákona o léčivech, jako organizační součást Státního ústavu pro kontrolu léčiv. Komunikace přes centrální úložiště povede ke vzniku systému elektronických lékových záznamů občanů a významně zefektivní a omezí rizika v komunikaci mezi lékaři, lékárnami a pacienty. Zároveň umožní výdej léčivých přípravků v nové kategorii výdeje bez lékařského předpisu s omezením.
The central repository of electronic prescriptions was established in confirmity to the Act on Pharmaceuticals as an organisational part of the State Institute for Drug Control. Communication via the central repository will lead to the creation of citizens' electronic drug records and will significantly limit risks of communication among doctors, pharmacies and patients. It will also make possible to launch a new category of medicinal products – medicinal products dispensed over-the-counter with restriction.
- MeSH
- databáze jako téma využití MeSH
- elektronické předepisování MeSH
- formuláře a záznamy - kontrola a vedení metody využití MeSH
- internet trendy využití MeSH
- kontrola léčiv a omamných látek metody zákonodárství a právo MeSH
- léčivé přípravky ekonomika MeSH
- lékárny ekonomika organizace a řízení trendy MeSH
- lékařská elektronika metody trendy zákonodárství a právo MeSH
- lékové předpisy ekonomika normy MeSH
- lidé MeSH
- zákonodárství lékové využití MeSH
- Check Tag
- lidé MeSH
