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Owing to their central role in the initiation and regulation of antitumor immunity, dendritic cells (DCs) have been widely tested for use in cancer immunotherapy. Despite several encouraging clinical applications, existing DC-based immunotherapy efforts have yielded inconsistent results. Recent work has identified strategies that may allow for more potent DC-based vaccines, such as the combination with antitumor agents that have the potential to synergistically enhance DC functions. Selected cytotoxic agents may stimulate DCs either by directly promoting their maturation or through the induction of immunogenic tumor cell death. Moreover, they may support DC-induced adaptive immune responses by disrupting tumor-induced immunosuppressive mechanisms via selective depletion or inhibition of regulatory subsets, such as myeloid-derived suppressor cells and/or regulatory T cells (Tregs). Here, we summarize our current knowledge on the capacity of anticancer chemotherapeutics to modulate DC phenotype and functions and the results of ongoing clinical trials evaluating the use of DC-based immunotherapy in combination with chemotherapy in cancer patients.
- MeSH
- antitumorózní látky terapeutické užití MeSH
- dendritické buňky imunologie MeSH
- imunoterapie MeSH
- klinické zkoušky jako téma MeSH
- lidé MeSH
- protinádorové vakcíny imunologie MeSH
- vakcinace * MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- přehledy MeSH
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- Publikační typ
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- Publikační typ
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Dendritic cells (DCs) are specific antigen-presenting cells that play critical roles in the initiation and polarization of immune responses. DCs residing in the lungs might be detected in the bronchoalveolar lavage fluid (BALF). We analysed DC compartment in the peripheral blood and BALF of patients with allergy and in controls. Plasmacytoid and four distinct subsets of myeloid DCs [characterized by the expression of blood dendritic cell antigen (BDCA)-1+ and -3+ and CD16 positivity or negativity] were detected in both tested compartments. We further evaluated the expression of C-type lectins [mannose receptor (MR), dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin (DC-SIGN) and dendritic and epithelial cells (DEC)-205] relevant to the pathogenesis of asthma. Interestingly, we found a selective increase in the frequency of myeloid DC-expressing BDCA-3 and MR particularly in BALF from allergic patients. Specific and highly statistically significant increase in BDCA-3+ and/or MR+ DCs brings a novel characteristic to BAL analysis in allergic patients.
- MeSH
- bronchiální astma krev imunologie MeSH
- bronchoalveolární lavážní tekutina cytologie imunologie MeSH
- dendritické buňky cytologie imunologie MeSH
- dítě MeSH
- dospělí MeSH
- GPI-vázané proteiny krev imunologie MeSH
- imunofenotypizace metody MeSH
- lektiny typu C krev imunologie MeSH
- lidé MeSH
- molekuly buněčné adheze krev imunologie MeSH
- neparametrická statistika MeSH
- plíce cytologie imunologie MeSH
- průtoková cytometrie MeSH
- receptory buněčného povrchu krev imunologie MeSH
- receptory IgG krev imunologie MeSH
- Check Tag
- dítě MeSH
- dospělí MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Hyper-immunoglobulin (Ig)E syndrome (HIES) is a primary immunodeficiency associated with mutations in STAT3 resulting in impaired development of T helper type 17 (Th17) lymphocytes. HIES patients with a reduced frequency of Th17 cells present with infections caused by Staphylococcus aureus and/or Candida strains. The same spectrum of pathogens is present in patients with chronic granulomatous disease (CGD).We analysed the characteristics of the Th17 compartment in HIES and CGD. HIES patients showed very low numbers of Th17 cells. By contrast, the frequency of Th17 cells and production of Th17-derived cytokines was significantly higher among CGD patients when compared to both control samples and HIES. Naive CD4(+) cells in CGD patients had a normal capacity to differentiate into IL-17-producing cells and the numbers of Th17 cells in the CGD patients normalized following successful bone marrow transplantation. Our findings complement recent data on the importance of Th17 cells for elimination of infections with C. albicans and S. aureus.
- MeSH
- buněčná diferenciace imunologie MeSH
- buňky Th17 imunologie metabolismus patologie MeSH
- Candida albicans růst a vývoj MeSH
- chronická granulomatózní nemoc genetika imunologie metabolismus patologie terapie MeSH
- dítě MeSH
- dospělí MeSH
- ELISA MeSH
- homologní transplantace MeSH
- interferon gama imunologie metabolismus MeSH
- interleukin-17 imunologie metabolismus MeSH
- Jobův syndrom genetika imunologie metabolismus patologie MeSH
- kandidóza imunologie mikrobiologie MeSH
- lidé MeSH
- membránové glykoproteiny genetika imunologie metabolismus MeSH
- mutace MeSH
- NADPH-oxidasy genetika imunologie metabolismus MeSH
- počet lymfocytů MeSH
- předškolní dítě MeSH
- průtoková cytometrie MeSH
- stafylokokové infekce imunologie mikrobiologie MeSH
- Staphylococcus aureus růst a vývoj MeSH
- studie případů a kontrol MeSH
- transkripční faktor STAT3 genetika imunologie metabolismus MeSH
- transplantace hematopoetických kmenových buněk MeSH
- Check Tag
- dítě MeSH
- dospělí MeSH
- lidé MeSH
- mužské pohlaví MeSH
- předškolní dítě MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
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