Our goal was to target silencing of the Plum pox virus coat protein (PPV CP) gene independently expressed in plants. Clone C-2 is a transgenic plum expressing CP. We introduced and verified, in planta, the effects of the inverse repeat of CP sequence split by a hairpin (IRSH) that was characterized in the HoneySweet plum. The IRSH construct was driven by two CaMV35S promoter sequences flanking the CP sequence and had been introduced into C1738 plum. To determine if this structure was enough to induce silencing, cross-hybridization was made with the C1738 clone and the CP expressing but PPV-susceptible C2 clone. In total, 4 out of 63 clones were silenced. While introduction of the IRSH is reduced due to the heterozygous character in C1738 plum, the silencing induced by the IRSH PPV CP is robust. Extensive studies, in greenhouse containment, demonstrated that the genetic resource of C1738 clone can silence the CP production. In addition, these were verified through the virus transgene pyramiding in the BO70146 BlueByrd cv. plum that successfully produced resistant BlueByrd BO70146 × C1738 (HybC1738) hybrid plums.
- MeSH
- Biotechnology methods MeSH
- Genetic Engineering methods MeSH
- Disease Resistance * MeSH
- Prunus genetics virology MeSH
- Transgenes MeSH
- Gene Silencing * MeSH
- Capsid Proteins genetics metabolism MeSH
- Plum Pox Virus genetics pathogenicity MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
According to Darwin's theory, endless evolution leads to a revolution. One such example is the Clustered Regularly Interspaced Palindromic Repeats (CRISPR)-Cas system, an adaptive immunity system in most archaea and many bacteria. Gene editing technology possesses a crucial potential to dramatically impact miscellaneous areas of life, and CRISPR-Cas represents the most suitable strategy. The system has ignited a revolution in the field of genetic engineering. The ease, precision, affordability of this system is akin to a Midas touch for researchers editing genomes. Undoubtedly, the applications of this system are endless. The CRISPR-Cas system is extensively employed in the treatment of infectious and genetic diseases, in metabolic disorders, in curing cancer, in developing sustainable methods for fuel production and chemicals, in improving the quality and quantity of food crops, and thus in catering to global food demands. Future applications of CRISPR-Cas will provide benefits for everyone and will save countless lives. The technology is evolving rapidly; therefore, an overview of continuous improvement is important. In this review, we aim to elucidate the current state of the CRISPR-Cas revolution in a tailor-made format from its discovery to exciting breakthroughs at the application level and further upcoming trends related to opportunities and challenges including ethical concerns.
- MeSH
- Archaea metabolism MeSH
- Bacteria metabolism MeSH
- CRISPR-Cas Systems * MeSH
- History, 20th Century MeSH
- History, 21st Century MeSH
- Livestock MeSH
- Gene Editing methods MeSH
- Genetic Engineering history methods MeSH
- Genome MeSH
- Humans MeSH
- Clustered Regularly Interspaced Short Palindromic Repeats MeSH
- Crops, Agricultural genetics MeSH
- Animals MeSH
- Check Tag
- History, 20th Century MeSH
- History, 21st Century MeSH
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Historical Article MeSH
- Review MeSH
- MeSH
- Genetic Engineering methods MeSH
- Organisms, Genetically Modified * genetics MeSH
- Gene Library MeSH
- Genes genetics MeSH
- Interdisciplinary Research methods MeSH
- Mice genetics MeSH
- Research MeSH
- Check Tag
- Mice genetics MeSH
- Publication type
- Newspaper Article MeSH
- Geographicals
- Czech Republic MeSH
We report for the first time, a comparison of two approaches for artificially induced triploidy in zebrafish (Danio rerio) using cold shock and heat shock treatments. Of the two methods, heat shock treatment proved more effective with a triploid production rate of 100% in particular females. Subsequently, triploid zebrafish larvae were used as recipients for intraperitoneal transplantation of ovarian and testicular cells originating from vas:EGFP strain in order to verify their suitability for surrogate reproduction. Production of donor-derived sperm was achieved in 23% of testicular cell recipients and 16% of ovarian cell recipients, indicating the suitability of triploids as surrogate hosts for germ cell transplantation. Success of the transplantation was confirmed by positive GFP signal detected in gonads of dissected fish and stripped sperm. Germline transmission was confirmed by fertilization tests followed by PCR analysis of embryos with GFP specific primers. Reproductive success of germline chimera triploids evaluated as fertilization rate and progeny development was comparable to control groups.
- MeSH
- Breeding methods MeSH
- Zebrafish genetics MeSH
- Genetic Engineering methods veterinary MeSH
- Flow Cytometry MeSH
- Temperature MeSH
- Triploidy * MeSH
- Germ Cells transplantation MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Comparative Study MeSH
- MeSH
- Biological Therapy ethics methods MeSH
- Targeted Gene Repair ethics methods MeSH
- CRISPR-Cas Systems genetics MeSH
- Gene Editing * ethics methods MeSH
- Fertilization in Vitro methods MeSH
- Genetic Therapy * ethics methods MeSH
- Genetic Engineering ethics methods MeSH
- HIV genetics MeSH
- Humans MeSH
- Receptors, CCR5 genetics MeSH
- Check Tag
- Humans MeSH
- Publication type
- Newspaper Article MeSH
Fakulta vojenského zdravotnictví Univerzity obrany, Hradec Králové Proteasy jsou skupina enzymů mikrobiálního, rostlinného i živočišného původu s nezastupitelným významem v základním metabolismu. Jedná se však i o materiál, který je vhodný k biotechnologickým aplikacím v širokém spektru oblastí jako je potravinový průmysl, výroba čistících prostředků a medicínské využití. Tento přehledový článek shrnuje základní informace o proteasach a nastiňuje příklady jejich použití.
Proteases are enzymes microbial, plant or animal origin with crucial importance in the basic metabolism. It is also a suitable material for biotechnological applications in a wide range of fields of interests like food industry, cleaning agents and medicine applications. This review paper surveys basic data about proteases and provides representative examples of their application.
- MeSH
- Biomedical Technology methods trends MeSH
- Biotechnology * methods trends MeSH
- Chemical Industry methods trends MeSH
- Enzymes chemistry metabolism MeSH
- Drug Industry methods trends MeSH
- Genetic Engineering methods trends utilization MeSH
- Microorganisms, Genetically-Modified enzymology genetics metabolism MeSH
- Hydrolysis * drug effects MeSH
- Humans MeSH
- Peptides chemistry metabolism MeSH
- Food Industry methods trends MeSH
- Peptide Hydrolases * chemistry metabolism MeSH
- Proteins chemistry metabolism MeSH
- Statistics as Topic MeSH
- Agriculture methods trends MeSH
- Check Tag
- Humans MeSH
- Publication type
- Review MeSH
- Keywords
- luspatercept,
- MeSH
- Anemia * therapy MeSH
- Receptors, Chimeric Antigen therapeutic use MeSH
- Genetic Engineering methods MeSH
- Immunoglobulin Fc Fragments therapeutic use MeSH
- Congresses as Topic MeSH
- Humans MeSH
- Recombinant Fusion Proteins therapeutic use MeSH
- Check Tag
- Humans MeSH
- Publication type
- News MeSH
