The former perception of the urothelium as an impermeable barrier has been revised during the last decade, as increasing evidence of changes in urine composition during its passage of the urinary tract has been presented. Since differences in urothelial permeability between upper and lower urinary tract have been found, our aim is to demonstrate whether changes in urine composition occur during passage through the ureter. We studied consecutive urine samples from both renal pelvises in six pigs and compared them to samples from the bladder and distal ureter. We further sampled urine during storage in the bladder at a fixed volume. All samples were analysed by measuring osmolality and pH, along with the concentration of the following parameters: Na(+), K(+), Cl(-), creatinine, urea. Urine alkalinity increased significantly during passage of the ureter. Creatinine concentration, pH and K(+) increased significantly during the passage from pelvis to the bladder. All other parameters increased non-significantly during the passage to the bladder. The increase in concentration was more pronounced at low concentrations in the pelvis. During storage in the bladder, there was a significant increase in urea concentration. Changes in the composition of urine occur during its passage from the renal pelvis to the bladder and during storage in the bladder. Despite the brief transit time, significant changes in alkalinity were found already during passage through the ureter.
- MeSH
- časové faktory MeSH
- chloridy moč MeSH
- draslík moč MeSH
- koncentrace vodíkových iontů MeSH
- koncentrační schopnost ledvin * MeSH
- kreatinin moč MeSH
- moč chemie MeSH
- močovina moč MeSH
- močový měchýř metabolismus MeSH
- osmolární koncentrace MeSH
- sodík moč MeSH
- Sus scrofa MeSH
- ureter metabolismus MeSH
- zvířata MeSH
- Check Tag
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- srovnávací studie MeSH
Pseudo-Bartterov syndróm je zriedkavá, ale dobre známa komplikácia cystickej fibrózy, charakterizovaná hypochloremickou hypokaliemickou metabolickou alkalózou. Autori opisujú zaujímavú kazuistiku 3-mesačného dojčaťa s negatívnym novorodeneckým skríningom a závažnou poruchou acidobázy a elektrolytov, ktorá viedla k podozreniu na cystickú fibrózu. Molekulovo-genetickým vyšetrením boli identifikované obidve mutácie CFTR génu (F508del/Glu831X). Pozoruhodné je, že ide o prvé dieťa rómskeho etnika s cystickou fibrózou na Slovensku. Pri pátraní po príčine hypochloremickej alkalózy treba vylúčiť cystickú fibrózu aj u dojčiat s negatívnym novorodeneckým skríningom.
Pseudo-Bartter syndrome is a rare but well known complication of cystic fibrosis characterized by hypochloremic hypokaliemic metabolic alkalosis. The authors describe an interesting case of a 3-month infant with negative newborn screening presented by severe acid-base and electrolytes disturbances, which led us to suspicion of the diagnosis of cystic fibrosis. Molecular-genetic testing identified both mutations of the CFTR gene (F508del/Glu831X). Remarkable is, that our patient is the first gypsy child with cystic fibrosis in Slovakia. In the searching for the cause of hypochloremic alkalosis, it is necessary to exclude cystic fibrosis also in infants with negative newborn screening.
- MeSH
- alkalóza * etiologie MeSH
- Bartterův syndrom diagnóza MeSH
- biochemická analýza krve MeSH
- chloridy moč MeSH
- cholecystolitiáza etiologie genetika ultrasonografie MeSH
- cystická fibróza * diagnóza patofyziologie prevence a kontrola MeSH
- diferenciální diagnóza MeSH
- elektrolyty MeSH
- infekce dýchací soustavy * etiologie patofyziologie MeSH
- klinické laboratorní techniky MeSH
- lidé MeSH
- novorozenec MeSH
- poruchy metabolismu vápníku etiologie MeSH
- pot MeSH
- protein CFTR MeSH
- Romové MeSH
- sodík moč MeSH
- vodní a elektrolytová nerovnováha * MeSH
- vrozené poruchy metabolismu metabolismus MeSH
- žlučové kameny etiologie MeSH
- Check Tag
- lidé MeSH
- novorozenec MeSH
- ženské pohlaví MeSH
- Publikační typ
- kazuistiky MeSH
