- MeSH
- 1-alfa-hydroxylasa 25-hydroxyvitaminu D3 izolace a purifikace krev MeSH
- Anguilliformes MeSH
- hydroxycholekalciferoly izolace a purifikace krev metabolismus MeSH
- hydroxylace fyziologie imunologie účinky záření MeSH
- játra enzymologie metabolismus MeSH
- ledviny enzymologie metabolismus MeSH
- lidé MeSH
- losos MeSH
- metabolické nemoci kostí diagnóza etiologie prevence a kontrola MeSH
- metabolismus fyziologie účinky léků účinky záření MeSH
- nedostatek vitaminu D * diagnóza etiologie prevence a kontrola MeSH
- potravní doplňky využití MeSH
- ryby klasifikace MeSH
- směrnice pro lékařskou praxi jako téma MeSH
- statistika jako téma MeSH
- ultrafialové záření * klasifikace MeSH
- vitamin D * fyziologie izolace a purifikace normy MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- kazuistiky MeSH
In this work, we studied model stratum corneum lipid mixtures composed of the hydroxylated skin ceramides N-lignoceroyl 6-hydroxysphingosine (Cer[NH]) and α-hydroxylignoceroyl phytosphingosine (Cer[AP]). Two model skin lipid mixtures of the composition Cer[NH] or Cer[AP], N-lignoceroyl sphingosine (Cer[NS]), lignoceric acid (C24:0) and cholesterol in a 0.5:0.5:1:1 molar ratio were compared. Model membranes were investigated by differential scanning calorimetry and 2H solid-state NMR spectroscopy at temperatures from 25 °C to 80 °C. Each component of the model mixture was specifically deuterated for selective detection by 2H NMR. Thus, the exact phase composition of the mixture at varying temperatures could be quantified. Moreover, using X-ray powder diffraction we investigated the lamellar phase formation. From the solid-state NMR and DSC studies, we found that both hydroxylated Cer[NH] and Cer[AP] exhibit a similar phase behavior. At physiological skin temperature of 32 °C, the lipids form a crystalline (orthorhombic) phase. With increasing temperature, most of the lipids become fluid and form a liquid-crystalline phase, which converts to the isotropic phase at higher temperatures (65-80 °C). Interestingly, lignoceric acid in the Cer[NH]-containing mixture has a tendency to form two types of fluid phases at 65 °C. This tendency was also observed in Cer[AP]-containing membranes at 80 °C. While Cer[AP]-containing lipid models formed a short periodicity phase featuring a repeat spacing of d = 5.4 nm, in the Cer[NH]-based model skin lipid membranes, the formation of unusual long periodicity phase with a repeat spacing of d = 10.7 nm was observed.
- MeSH
- biologické modely MeSH
- ceramidy chemie metabolismus MeSH
- cholesterol chemie MeSH
- deuterium chemie MeSH
- hydroxylace fyziologie MeSH
- kůže chemie metabolismus MeSH
- lidé MeSH
- lipidové dvojvrstvy chemie metabolismus MeSH
- magnetická rezonanční spektroskopie metody MeSH
- permeabilita buněčné membrány MeSH
- prášková difrakce metody MeSH
- rentgenové záření MeSH
- teplota kůže fyziologie MeSH
- teplota MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- MeSH
- hydroxylace fyziologie MeSH
- lidé MeSH
- metabolická inaktivace MeSH
- psychofarmakologie MeSH
- Check Tag
- lidé MeSH
