Q30322455
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- MeSH
- antioxidancia * analýza MeSH
- elektrochemické techniky * metody využití MeSH
- klinické laboratorní techniky metody trendy MeSH
- lidé MeSH
- oxidační stres MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- práce podpořená grantem MeSH
- přehledy MeSH
The application of nanotechnology in tissue engineering is an emerging and rapidly growing area of research. The advances of nanotechnology can bring additional functionality to vascular scaffolds and optimize internal vascular graft surface. Metals and their salts are used for their antibacterial effects, Ag(I) and Cu(II) ions ranking among the most studied ones. Recently antibacterial effects of Pt, Pd and Rh have also been demostrated. Natural polymers are also suitable for modification and improvement of the properties of vascular grafts, chitosan and hyaluronic acid being their main representatives. In addition to antimicrobial effects they support cells endothelisation and increase vascular permeability.
- MeSH
- biokompatibilní materiály MeSH
- biotechnologie * metody MeSH
- cévní protézy * trendy MeSH
- chitosan MeSH
- elastin MeSH
- katetrizace * MeSH
- katétry MeSH
- kolagen MeSH
- kovové nanočástice * využití MeSH
- kyselina hyaluronová MeSH
- lidé MeSH
- nanostruktury využití MeSH
- nanotechnologie * metody MeSH
- nanovlákna MeSH
- průchodnost cév * MeSH
- tkáňové inženýrství * MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- práce podpořená grantem MeSH
In the food industry, in the process of creating new agricultural plant products, and in the testing of anti-cancer drugs there is often a need to assay multiple samples of low molecular weight antioxidants, plant samples and foods rich in antioxidants, with minimal additional costs and low degrees of uncertainty. With these demands in mind, we decided to study the fully automated assay of antioxidants using not only automated sample measurements but also automated processing of samples and application of reagents. The automated pipetting system epMotion 5075 and the automated spectrophotometer BS 400 were chosen for the assay purposes. Five methods were introduced for the automation: 2-diphenyl-1-picrylhydrazyl (DPPH) test, ferric reducing antioxidant power (FRAP) method, 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) based test, N,N-dimethyl-1,4-diaminobenzene (DMPD) based test and the free radicals method. Samples containing one of the four antioxidants (standard rutin, quercitrin, ferulic and gallic acid) in a range 1–1000 μg/ml were used throughout. All of the tested methods were found suitable for implementation in an automated assay. However, some of them, such as the ABTS test failed to assay all tested antioxidants. The coefficients of determination were also unequal. From the analytical point of view, FRAP methods provided the most reliable results in the automated assay; because of the capacity of the method, approximately 240 samples per hour (one sample per 15 seconds) can be assayed using the automated protocol. We were encouraged by the data received and we expect further interest in the practical performance of such automation. As a mean of testing the robustness of our method, in the next step of our study, oxidative status was assessed in model cell lines derived from prostate cancer (PC-3, PNT1A and 22RV1) that were cultured on ellipticine (0, 0.5, 1, 1.5, 2, 2.5, 5, 7.5, 10, 15 μmol/l) supplemented agar. Antioxidant activity was assessed (DPPH, ABTS, FRAP, DMPD, FR) and calculated on the phenolic antioxidant level (rutin, quercitrin, ferulic and gallic acid), and thus an estimation was formulated of the oxidative stress as a result of the impact of anti-cancer drugs. It can be demonstrated that the new method has wide applicability.
- MeSH
- antioxidancia analýza MeSH
- chemické techniky analytické metody přístrojové vybavení statistika a číselné údaje MeSH
- elipticiny analýza chemie metabolismus MeSH
- financování organizované MeSH
- FRAP MeSH
- kalibrace MeSH
- kyselina gallová analýza chemie MeSH
- kyseliny kumarové analýza chemie MeSH
- laboratorní automatizace metody přístrojové vybavení MeSH
- luminiscenční měření MeSH
- nádorové buněčné linie MeSH
- oxidační stres účinky léků MeSH
- protinádorové látky chemie metabolismus toxicita MeSH
- quercetin analogy a deriváty analýza chemie MeSH
- reprodukovatelnost výsledků MeSH
- rutin analýza chemie MeSH
- screeningové testy protinádorových léčiv metody statistika a číselné údaje MeSH
- spektrofotometrie metody přístrojové vybavení statistika a číselné údaje MeSH
- viabilita buněk účinky léků MeSH
- volné radikály analýza MeSH
- vztahy mezi strukturou a aktivitou MeSH
- Publikační typ
- hodnotící studie MeSH
- statistiky MeSH
- tabulky MeSH
Currently, the influenza virus infects millions of individuals every year. Since the influenza virus represents one of the greatest threats, it is necessary to develop a diagnostic technique that can quickly, inexpensively, and accurately detect the virus to effectively treat and control seasonal and pandemic strains. This study presents an alternative to current detection methods. The flow-injection analysis-based biosensor, which can rapidly and economically analyze a wide panel of influenza virus strains by using paramagnetic particles modified with glycan, can selectively bind to specific viral A/H5N1/Vietnam/1203/2004 protein-labeled quantum dots. Optimized detection of cadmium sulfide quantum dots (CdS QDs)-protein complexes connected to paramagnetic microbeads was performed using differential pulse voltammetry on the surface of a hanging mercury drop electrode (HMDE) and/or glassy carbon electrode (GCE). Detection limit (3 S/N) estimations based on cadmium(II) ions quantification were 0.1 μg/mL or 10 μg/mL viral protein at HMDE or GCE, respectively. Viral protein detection was directly determined using differential pulse voltammetry Brdicka reaction. The limit detection (3 S/N) of viral protein was estimated as 0.1 μg/mL. Streptavidin-modified paramagnetic particles were mixed with biotinylated selective glycan to modify their surfaces. Under optimized conditions (250 μg/mL of glycan, 30-min long interaction with viral protein, 25°C and 400 rpm), the viral protein labeled with quantum dots was selectively isolated and its cadmium(II) content was determined. Cadmium was present in detectable amounts of 10 ng per mg of protein. Using this method, submicrogram concentrations of viral proteins can be identified.
- MeSH
- biosenzitivní techniky přístrojové vybavení metody MeSH
- biotin chemie metabolismus MeSH
- elektrochemické techniky přístrojové vybavení metody MeSH
- elektrody MeSH
- hemaglutininové glykoproteiny viru chřipky analýza metabolismus MeSH
- kadmium analýza MeSH
- kvantové tečky MeSH
- limita detekce MeSH
- lineární modely MeSH
- magnetické nanočástice chemie MeSH
- průtoková injekční analýza přístrojové vybavení metody MeSH
- rtuť chemie MeSH
- sloučeniny kadmia chemie MeSH
- streptavidin chemie metabolismus MeSH
- sulfidy chemie MeSH
- uhlík chemie MeSH
- virus chřipky A, podtyp H5N1 chemie izolace a purifikace MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Klíčová slova
- ssDNA, square-ware voltametrie,
- MeSH
- antracykliny * aplikace a dávkování škodlivé účinky terapeutické užití MeSH
- cytostatické látky terapeutické užití MeSH
- doxorubicin MeSH
- elektrochemické techniky metody využití MeSH
- hodnotící studie jako téma MeSH
- lékové interakce * MeSH
- lidé MeSH
- oligonukleotidy biosyntéza MeSH
- Check Tag
- lidé MeSH
- Klíčová slova
- square-ware voltametrie,
- MeSH
- antracykliny * aplikace a dávkování škodlivé účinky terapeutické užití MeSH
- cytostatické látky terapeutické užití MeSH
- doxorubicin MeSH
- elektrochemické techniky metody využití MeSH
- hodnotící studie jako téma MeSH
- lékové interakce * MeSH
- lidé MeSH
- oligonukleotidy biosyntéza MeSH
- Check Tag
- lidé MeSH
Guanosine derivatives are important for diagnosis of oxidative DNA damage including 8-hydroxy-2'-deoxyguanosine (8-OHdG) as one of the most abundant products of DNA oxidation. This compound is commonly determined in urine, which makes 8-OHdG a good non-invasive marker of oxidation stress. In this study, we optimized and tested the isolation of 8-OHdG from biological matrix by using paramagnetic particles with an antibody-modified surface. 8-OHdG was determined using 1-naphthol generated by alkaline phosphatase conjugated with the secondary antibody. 1-Naphthol was determined by stopped flow injection analysis (SFIA) with electrochemical detector using a glassy carbon working electrode and by stationary electrochemical detection using linear sweep voltammetry. A special modular electrochemical SFIA system which needs only 10 μL of sample including working buffer for one analysis was completely designed and successfully verified. The recoveries in different matrices and analyte concentration were estimated. Detection limit (3 S/N) was estimated as 5 pg/mL of 8-OHdG. This method promises to be very easily modified to microfluidic systems as "lab on valve". The optimized method had sufficient selectivity and thus could be used for determination of 8-OHDG in human urine and therefore for estimation of oxidative DNA damage as a result of oxidation stress in prostate cancer patients.
- MeSH
- alkalická fosfatasa MeSH
- deoxyguanosin analogy a deriváty moč MeSH
- elektrochemické techniky metody MeSH
- ELISA metody MeSH
- lidé středního věku MeSH
- lidé MeSH
- magnety MeSH
- mikrofluidní analytické techniky přístrojové vybavení metody MeSH
- mikrosféry MeSH
- nádory prostaty moč MeSH
- naftoly MeSH
- oxidační stres MeSH
- protilátky MeSH
- průtoková injekční analýza MeSH
- robotika přístrojové vybavení metody MeSH
- senioři MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
