sv.
sv.
- MeSH
- parodontologie MeSH
- Publikační typ
- periodika MeSH
- Konspekt
- Stomatologie
- NLK Obory
- zubní lékařství
Influence of different forms of glutathione on Lactobacillus casei ssp. rhamnosus culture. Lactobacillus casei ssp. rhamnosus (L. rhamnosus) of the genus Lactobacillus belongs to the facultatively heterofermentative lactic acid bacteria. In the human body it occurs mainly in the intestine, where it brings a beneficial effect on the immune system. Further, the antioxidant properties and action against oxidative stress belong among its major advantages. In the genus Lactobacillus also the ability to reduce clinical symptoms of influenza was demonstrated. Similar properties to that of the genus Lactobacillus are demonstrated also in glutathione (γ-glutamylcysteinylglycin), one of the most important thiols present in both plant and animal tissues, and also in number of microorganisms. Due to the wide field of application of bacteria of the genus Lactobacillus and possibilities to improve some of their properties in the presence of glutathione, the use of lactobacilli with the addition of glutathione can be considered for the treatment of influenza and support the treatment of influenza illness.
- Klíčová slova
- redukovaný glutathion, oxidovaný glutathion,
- MeSH
- chřipka lidská farmakoterapie MeSH
- glutathion * metabolismus terapeutické užití MeSH
- glutathiondisulfid metabolismus MeSH
- Lacticaseibacillus rhamnosus * metabolismus MeSH
- lidé MeSH
- S-nitrosoglutathion metabolismus MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- práce podpořená grantem MeSH
- přehledy MeSH
- MeSH
- jedlé rostliny analýza anatomie a histologie MeSH
- léčivé rostliny analýza anatomie a histologie MeSH
- Publikační typ
- srovnávací studie MeSH
Meiotic maturation of oocyte relies on pre-synthesised maternal mRNA, the translation of which is highly coordinated in space and time. Here, we provide a detailed polysome profiling protocol that demonstrates a combination of the sucrose gradient ultracentrifugation in small SW55Ti tubes with the qRT-PCR-based quantification of 18S and 28S rRNAs in fractionated polysome profile. This newly optimised method, named Scarce Sample Polysome Profiling (SSP-profiling), is suitable for both scarce and conventional sample sizes and is compatible with downstream RNA-seq to identify polysome associated transcripts. Utilising SSP-profiling we have assayed the translatome of mouse oocytes at the onset of nuclear envelope breakdown (NEBD)-a developmental point, the study of which is important for furthering our understanding of the molecular mechanisms leading to oocyte aneuploidy. Our analyses identified 1847 transcripts with moderate to strong polysome occupancy, including abundantly represented mRNAs encoding mitochondrial and ribosomal proteins, proteasomal components, glycolytic and amino acids synthetic enzymes, proteins involved in cytoskeleton organization plus RNA-binding and translation initiation factors. In addition to transcripts encoding known players of meiotic progression, we also identified several mRNAs encoding proteins of unknown function. Polysome profiles generated using SSP-profiling were more than comparable to those developed using existing conventional approaches, being demonstrably superior in their resolution, reproducibility, versatility, speed of derivation and downstream protocol applicability.
- MeSH
- jaderný obal genetika metabolismus MeSH
- meióza genetika MeSH
- myši MeSH
- oocyty růst a vývoj metabolismus MeSH
- polyribozomy genetika MeSH
- proteiny vázající RNA genetika MeSH
- RNA messenger skladovaná genetika MeSH
- RNA ribozomální 18S genetika MeSH
- RNA ribozomální 28S genetika MeSH
- sekvenování transkriptomu MeSH
- vývojová regulace genové exprese genetika MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
