Lactoperoxidase (LP) is an important enzyme of the salivary and mammary glands. It has been proven to increase the shelf life of raw milk by inhibiting the growth of bacteria, especially Listeria monocytogenes, Escherichia coli, Staphylococcus aureus, and Pseudomonas spp. The aim of this work was to verify the use of LP to extend the shelf life of meat products. In vitro experiments showed inhibitory effects on the selected bacteria (Listeria innocua (ATCC 33090), Staphylococcus saprophyticus (CP054440.1), and Pseudomonas fluorescens (ATCC 13525) due to a prolongation of the lag phase of growth curves. A lower increase in viable counts (p < 0.05) was also found by testing pork cubes' surface treated with LP solution (5%) + L. innocua and stored for 7 days at 15 °C. LP has also been studied at concentrations of 0.25 and 0.50% in meat products (pork ham and pâté) during refrigerated storage (4 °C for 28 days). Lower viable counts were observed throughout the storage experiment, especially for 0.50% LP (p < 0.05). Meat products containing LP also showed lower levels of oxidation (MAD) (p < 0.05). According to these results, LP could extend the shelf life of a wider range of products.

• Publikační typ
• časopisecké články MeSH

The virulence factors, antibiotic resistance patterns, and the associated genetic elements have been investigated in Staphylococcus species. A total of 100 strains has been isolated from clinical samples in the Microbiology Laboratory of Hesperia Hospital, Modena, Italy, and identified as Staphylococcus aureus (65), Staphylococcus epidermidis (24), Staphylococcus hominis (3), Staphylococcus saprophyticus (3), and Staphylococcus warneri (5). All the strains were analyzed to determine phenotypic and genotypic characters, notably the virulence factors, the antibiotics susceptibility, and the genetic determinants. The highest percentage of resistance in Staphylococcus spp. was found for erythromycin and benzylpenicillin (87% and 85%, respectively). All S. aureus, two S. epidermidis (8.3%), and one S. saprophyticus (33.3%) strains were resistant to oxacillin. The methicillin resistance gene (mecA) was detected by polymerase chain reaction (PCR) amplification in 65 S. aureus strains and in 3 coagulase-negative staphylococci (CoNS) (8.6%). With regard to the virulence characteristics, all the S. aureus were positive to all virulence tests, except for slime test. Among the CoNS isolates, 19 (79.1%) S. epidermidis and one (33.3%) S. saprophyticus strains resulted positive for the slime test only. The results obtained are useful for a more in-depth understanding of the function and contribution of S. aureus and CoNS antibiotic resistance and virulence factors to staphylococcal infections. In particular, the production of slime is very important for CoNS, a virulence factor frequently found in infections caused by these strains. Further investigations on the genetic relatedness among strains of different sources will be useful for epidemiological and monitoring purposes and will enable us to develop new strategies to counteract the diffusion of methicillin-resistant S. aureus (MRSA) and CoNS strains not only in clinical field, but also in other related environments.

• MeSH
• antibakteriální látky * farmakologie   MeSH
• bakteriální léková rezistence * MeSH
• bakteriální proteiny genetika   MeSH
• faktory virulence * genetika   MeSH
• lidé MeSH
• mikrobiální testy citlivosti * MeSH
• stafylokokové infekce * mikrobiologie   MeSH
• Staphylococcus * genetika   účinky léků   izolace a purifikace   patogenita   klasifikace   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Itálie MeSH

Cervical lymphadenopathy in children usually has an infectious or reactive etiology, but patients with persistent findings, adolescent age and accompanying systemic symptoms or abnormal laboratory findings should undergo an early biopsy. In our fifteen-year-old girl, who observed cervical lymphomegaly for 4 weeks without improvement, Staphylococcus saprophyticus was cultured from a peroperative biopsy. Coagulase-negative staphylococci (CoNS) represent the main part of the microflora of the skin and mucous membranes of humans, but they can also be used as important opportunistic pathogens.

• MeSH
• antibakteriální látky aplikace a dávkování   terapeutické užití   MeSH
• hnisání MeSH
• koagulasa MeSH
• krk * chirurgie   patologie   MeSH
• lidé MeSH
• lymfadenitida * chirurgie   diagnóza   terapie   MeSH
• mladiství MeSH
• stafylokokové infekce diagnóza   farmakoterapie   MeSH
• Staphylococcus saprophyticus izolace a purifikace   MeSH
• Check Tag
• lidé MeSH
• mladiství MeSH
• ženské pohlaví MeSH
• Publikační typ
• kazuistiky MeSH

Vysokoškolská učebnice, která se zaměřuje na různé druhy mikroorganismů a jejich ekologii a patogenitu.

• MeSH
• ekologie MeSH
• infekční nemoci MeSH
• mikrobiologické jevy MeSH
• mikrobiologie životního prostředí MeSH
• molekulární biologie MeSH
• Publikační typ
• monografie MeSH

• Konspekt
• Mikrobiologie
• Učební osnovy. Vyučovací předměty. Učebnice
• NLK Obory
• mikrobiologie, lékařská mikrobiologie
• NLK Publikační typ
• učebnice vysokých škol

V článku je popsána analýza 2 449 kmenů koguláza negativních stafylokoků (KNS) izolovaných z humánního klinického materiálu, které byly zaslány do NRL pro stafylokoky k identifikaci v letech 2000–2020. Celkem bylo zjištěno 35 různých (pod)druhů KNS, nejčastěji kmeny Staphylococcus epidermidis, S. haemolyticus, S. hominis a S. lugdunensis, z močí potom S. saprophyticus. Dále byla identifikována řada stafylokoků vyskytujících se spíše ve veterinárním materiálu nebo v prostředí. Zachyceny byly i oxidáza pozitivní kmeny nově uvedeného rodu Mammaliicoccus (bývalá „S. sciuri group“).

In total, 2449 strains of coagulase-negative staphylococci (CNS) isolated from human clinical specimens were referred to the National Reference Laboratory for Staphylococci for identification in 2000–2020. Thirty-five CNS species and subspecies were identified, and the most frequent were Staphylococcus epidermidis, S. haemolyticus, S. hominis and S. lugdunensis. S.saprophyticus was the most common isolate from urine. A number of staphylococcal species normally detected in veterinary or environmental specimens were also recorded. In addition, oxidase-positive strains of the newly described genus Mammaliicoccus (former „S. sciuri group“) were identified.

Stafylokoky jsou ubikvitérní mikroorganismy vyskytující se běžně na předmětech denního užívání. Pouze některé ze 40 druhů a poddruhů se uplatňují v humánní medicíně. Největší význam pro člověka mají Staphylococcus aureus, S. epidermidis a S. saprophyticus. Výskyt stafylokoků na dětských hračkách u hospitalizovaných dětí není rutinně sledován. Zahraniční studie naznačují, že by mohly být zdrojem nákaz spojených se zdravotní péčí. Proto byla v letech 2016 a 2017 provedena šetření v pražských nemocnicích. Z výsledků šetření vyplývá, že záchyt stafylokoků na hračkách je ovlivněn zejména materiálem, ze kterého jsou hračky vyrobeny, a dále i prostředím, ve kterém jsou umístěny.

Staphylococci are ubiquitous microorganisms commonly found in daily use articles. Only some of the 40 species and subspecies are used in human medicine. The most important for humans are Staphylococcus aureus, S. epidermidis and S. saprophyticus.Information on the presence of staphylococci on children's toys in hospitalized children is not available on routine basis. Foreign studies conducted abroad suggest that the toys could be sources of healthcare-associated infections. Consequently a survey was carried out in Prague hospitals in 2016 and 2017. The results of the study show that capture of staphylococci on toys is influenced, in particular, by the material from which the toys are produced and also by the environment.

• MeSH
• bezpečnost pacientů MeSH
• dítě MeSH
• hospitalizace MeSH
• hra a hračky * MeSH
• infekce spojené se zdravotní péčí MeSH
• lidé MeSH
• stafylokokové infekce * přenos   prevence a kontrola   MeSH
• Staphylococcus MeSH
• výzkum MeSH
• Check Tag
• dítě MeSH
• lidé MeSH

Chronic wounds and their associated bacterial infections are major issues in modern health care systems. Therefore, antimicrobial resistance (AMR), treatment costs, and number of disability-adjusted life-years have gained more interest. Recently, photodynamic therapy emerged as an effective approach against resistant and naïve bacterial strains with a low probability of creating AMR. In this study, needleless electrospinning was used to produce an indocyanine green (ICG) loaded poly(d,l-lactide) nanofibrous mesh as a photoresponsive wound dressing. The non-woven mesh had a homogeneous nanofibrous structure and showed long-term hydrolytic stability at different pH values. The antimicrobial activity was tested against several bacterial strains, namely Staphylococcus saprophyticus subsp. bovis, Escherichia coli DH5 alpha, and Staphylococcus aureus subsp. aureus. Upon irradiation with a laser of a specific wavelength (λ = 810 nm), the bacterial viability was significantly reduced by 99.978% (3.66 log10), 99.699% (2.52 log10), and 99.977% (3.64 log10), respectively. The nanofibrous mesh showed good biocompatibility, which was confirmed by the proliferation of mouse fibroblasts (L929) on the surface and into deeper parts of the mesh. Furthermore, a favorable proangiogenic effect was observed in ovo using the chorioallantoic membrane assay. In general, it can be concluded that ICG loaded nanofibers as an innovative wound dressing represent a promising strategy against chronic wounds associated with skin infections.

• MeSH
• biokompatibilní materiály chemie   farmakologie   MeSH
• buněčné linie MeSH
• chorioalantoická membrána krevní zásobení   účinky léků   MeSH
• Escherichia coli účinky léků   růst a vývoj   MeSH
• fibroblasty cytologie   účinky léků   MeSH
• fotochemoterapie MeSH
• indokyanová zeleň chemie   farmakologie   MeSH
• mikrobiální viabilita účinky léků   MeSH
• myši MeSH
• nanovlákna MeSH
• obvazy MeSH
• polyestery chemie   MeSH
• proliferace buněk účinky léků   MeSH
• Staphylococcus aureus účinky léků   růst a vývoj   MeSH
• Staphylococcus saprophyticus účinky léků   růst a vývoj   MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH

OBJECTIVES: This study was aimed to assess the concentration of microbial aerosol and species composition of airborne staphylococci in 10 healthcare facilities in southern Poland including primary healthcare units and hospital wards; and to assess whether the selected components of microbial aerosol pose a threat of severe infections to either patients or the personnel. METHODS: The study was conducted at monthly intervals over a period of one year. Air samples were collected by MAS-100 sampler. The number of mesophilic bacteria, mould fungi, actinomycetes and staphylococci was determined on general and selective media. The species identification of staphylococci was conducted using API tests for strains that were pre-selected based on macroscopic and microscopic observations. RESULTS: A total number of 1,584 samples were collected during the sampling period. The numbers of airborne microorganisms varied between the examined premises and between the seasons of the year. The observed differences were statistically significant with one exception for actinomycetes and their differences between the examined premises. The concentrations of mesophilic bacteria varied from 5 to 297 CFU/m3 of air, for Staphylococcus the values ranged from 1 to 96 CFU/m3, for fungi - from 1 to 100 CFU/m3, and the number of actinomycetes ranged from 7 to 321 CFU/m3. Ten species of coagulase-negative staphylococci (CoNS) were identified among 55 isolates with S. saprophyticus and S. warneri being the most frequently detected (n = 14 and 13, respectively). S. haemolyticus, which is one of the most common causal agents of nosocomial infections was observed in four facilities (n = 5). CONCLUSIONS: The microbial concentrations varied both between the seasons of the year and between the examined facilities. The highest bioaerosol concentrations were observed in most crowded premises. The identified species of staphylococci, although not typically associated with human infections, are common causal agents of nosocomial infections and infections in immunocompromised people.

• MeSH
• aerosoly MeSH
• houby MeSH
• látky znečišťující vzduch v pracovním prostředí analýza   MeSH
• lidé MeSH
• mikrobiologie vzduchu * MeSH
• pracovní expozice * analýza   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Polsko MeSH

Antibiotic resistance is increasing among Staphylococcus saprophyticus strains isolated from urinary tract infection. This necessitates alternative therapies. For this, a lytic phage (vB_SsapS-104) against S. saprophyticus, which formed round and clear plaques on bacterial culture plates, was isolated from hospital wastewater and characterized. Microscopy analysis showed that it had a small head (about 50 nm), tail (about 80 nm), and a collar (about 22 nm in length and 19 nm in width) indicating to be a phage within Siphoviridae family. Phage vB_SsapS-104 showed a large latency period of about 40 min, rapid adsorption rate that was significantly enhanced by MgCl2 and CaCl2, and high stability to a wide range of temperatures and pH values. Restriction analyses demonstrated that phage consists of a double-stranded DNA with an approximate genome size of 40 Kb. BLAST results did not show high similarity (megablast) with other previously identified phages. But, in Blastn, similarity with Staphylococcus phages was observed. Phage vB_SsapS-104 represented high anti-bacterial activity against S. saprophyticus isolates in vitro as it was able to lyse 8 of the 9 clinical isolates (%88.8) obtained from a hospital in Gorgan, Iran. It was a S. saprophyticus-specific phage because no lytic activity was observed on some other pathogenic bacteria tested. Therefore, phage vB_SsapS-104 can be considered as a specific virulent phage against of S. saprophyitcus isolated from urinary tract infection. This study provided the partial genomic characterization of S. saprophyticus phage and its application against urinary tract infection associated with S. saprophyticus. This phage also can be considered as a good candidate for a therapeutic alternative in the future.

• MeSH
• antibakteriální látky farmakologie   MeSH
• DNA virů MeSH
• fágová terapie MeSH
• genom virový MeSH
• hostitelská specificita MeSH
• infekce močového ústrojí mikrobiologie   MeSH
• koncentrace vodíkových iontů MeSH
• latence viru MeSH
• lidé MeSH
• odpadní voda virologie   MeSH
• sekvenční analýza DNA MeSH
• Siphoviridae genetika   izolace a purifikace   ultrastruktura   MeSH
• stafylokokové bakteriofágy genetika   MeSH
• stafylokokové infekce mikrobiologie   MeSH
• Staphylococcus saprophyticus účinky léků   virologie   MeSH
• teplota MeSH
• transmisní elektronová mikroskopie MeSH
• virulence MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Írán MeSH

Byl popsán nový druh koaguláza-negativního stafylokoka. Staphylococcus edaphicus je novobiocin-rezistentní,oxidáza-negativní, podle fylogenetických vlastností je nejblíže k druhu Staphylococcus saprophyticus. Dva kme-ny nového druhu, které byly izolovány z prostředí na ostrově Jamese Rosse v Antarktidě, jsou rezistentník betalaktamovým antibiotikům. Je to již desátý stafylokok popsaný českými taxonomy.

A novel species of coagulase-negative Staphylococcus has been described. Staphylococcus edaphicus is resistant tonovobiocin, is oxidase negative, and has phylogenetic characteristics that are closest to those of Staphylococcussaprophyticus. Two strains of the novel species isolated from the environment on the James Ross Island in Antarcticaare resistant to beta-lactam antibiotics. This is the tenth staphylococcal species described by Czech taxonomists.

• MeSH
• bakteriální proteiny MeSH
• koagulasa MeSH
• methicilin rezistentní Staphylococcus aureus izolace a purifikace   MeSH
• Geografické názvy
• Antarktida MeSH