Cílem studie je zjistit kolonizaci dolního genitálního traktu Ureaplasma urealyticum u pacientek s předčasným odtokem plodové vody mezi 24. až 34. týdnem gestace. Typ studie: Retrospektivní studie. Název a sídlo pracoviště: Lékařská fakulta Hradec Králové, Univerzita Karlova Praha. Porodnická a gynekologická klinika FN Hradec Králové Metodika: U 150 pacientek s předčasným odtokem plodové vody mezi 24. až 34. týdnem gravidity byly provedeny standardní odběry z hrdla na anaerobní a aerobní kultivaci. U poloviny z nich byly provedeny i odběry na genitální mykoplazmata. Kontrolní skupinu tvoří pacientky s fyziologickým průběhem gravidity. Výsledky: Ureaplasma urealyticum byla zjištěna u 96 % (72/75) pacientek s předčasným odtokem plodové vody mezi 24. až 34. týdnem gestace a u 32 % (24/75) těhotných s fyziologickým průběhem gravidity. Závěr: Kolonizace dolního genitálního traktu Ureaplasma urealyticum může být spojena s předčasným odtokem plodové vody a chorioamniitidou.
The purpose of this study was to evaluate the prevalence of the maternal lower genital tract colonization by Ureaplasma urealiticum and Mycoplasma hominis in patiens with preterm premature rupture of the membranes. DESIGN: Retrospective study. SETTING: Department of Obstetrics and Gynecology Medical Faculty Charles University Hradec Králové. METHODS: We studied 150 women between 24 and 34 weeks of gestation with preterm premature rupture of the membranes. These patients were divided into 2 groups. In group 1 swabs were obtained for genital mycoplasmas. In both groups 1 and 2 were obtained standard swabs for aerobic and anaerobic cultivation. Control group 3 were women with normal pregnancy. RESULTS: Ureaplasma urealyticum was detected in 96% (72/75) of the patients with PPROM between 24 and 34 weeks of gestation and in 32% (24/75) of the patiens of the control group (normal pregnancy). CONCLUSION: The maternal lower genital tract colonization by Ureaplasma urealyticum might be associated with preterm premature rupture of the membranes and chorioamniitis.
- MeSH
- Cervix Uteri microbiology MeSH
- Pregnancy Complications, Infectious MeSH
- Humans MeSH
- Mycoplasma hominis isolation & purification MeSH
- Mycoplasma Infections complications MeSH
- Fetal Membranes, Premature Rupture microbiology MeSH
- Pregnancy MeSH
- Ureaplasma urealyticum isolation & purification MeSH
- Ureaplasma Infections diagnosis complications MeSH
- Check Tag
- Humans MeSH
- Pregnancy MeSH
- Female MeSH
- MeSH
- Chlamydia trachomatis MeSH
- Child MeSH
- Adult MeSH
- Infant MeSH
- Humans MeSH
- Mothers microbiology MeSH
- Mycoplasma hominis MeSH
- Infant, Newborn microbiology MeSH
- Pregnancy MeSH
- Ureaplasma MeSH
- Infectious Disease Transmission, Vertical statistics & numerical data MeSH
- Check Tag
- Child MeSH
- Adult MeSH
- Infant MeSH
- Humans MeSH
- Infant, Newborn microbiology MeSH
- Pregnancy MeSH
- Female MeSH
Cíle: Mycoplasma hominis a Ureaplasma urealyticum jsou podmíněně patogenní mikroorganismy, které se frekventovaně vyskytují v urogenitálním ústrojí žen. Uvedené bakteriální druhy mohou způsobovat infekce urogenitálního traktu a jsou rovněž spojovány s nepříznivým vlivem na průběh těhotenství, případně s lidskou neplodností. Cílem této práce bylo zjistit četnost výskytu M. hominis a U. urealyticum u žen z asistované reprodukce ve srovnání s kontrolní skupinou plodných žen. Metody: Průkaz mykoplazmat v cervikálních stěrech byl prováděn kultivačním vyšetřením a polymerázovou řetězovou reakcí. U izolovaných kmenů byla následně ověřena mikrodiluční bujónovou metodou jejich citlivost k azithromycinu, ciprofloxacinu, doxycyklinu a erythromycinu. Výsledky: Celkově bylo vyšetřeno 111 žen s poruchou reprodukční funkce. U. urealyticum bylo prokázáno ve stěrech 44 žen (39,6 %). Výskyt M. hominis byl zaznamenán pouze u 9 žen (8,1 %). Z tohoto počtu bylo 6 žen (5,4 %) pozitivních na přítomnost obou bakteriálních druhů. Kontrolní skupinu tvořilo 23 žen. Přítomnost U. urealyticum byla detekována u 8 žen (34,7 %). M. hominis bylo detekováno pouze ve směsi s U. urealyticum, a to ve 3 případech (13,0 %). Nejvyšší inhibiční aktivita na oba testované druhy byla pozorována u doxycyklinu. Závěr: Výsledky ukazují na vyšší incidenci M. hominis a U. urealyticum v genitálním traktu žen s problémy v reprodukci ve srovnání s kontrolní skupinou. Potenciální negativní efekt na reprodukční schopnost žen nebyl pozorován.
Aims: Mycoplasma hominis and Ureaplasma urealyticum are potentially pathogenic bacterial species that are frequently isolated from the urogenital tract of women. These pathogens could be responsible for various genitourinary diseases and have been associated with adverse pregnancy outcomes and female fertility problems. The aim of this study was to analyse the presence of M. hominis and U. urealyticum in the cervical canal of uterus of women with and without fertility problems. Methods: Endocervical swabs obtained from women with reproductive problems and fertile women were tested by both cultivation and polymerase chain reaction. The antimicrobial susceptibility to the azithromycin, ciprofloxacin, doxycycline and erythromycine of the isolated strains of M. hominis and U. urealyticum was also tested by the microdilution broth method. Results: A total of 111 women with fertile problems were examined. U. urealyticum was detected in samples from 44 (39.6%) women. M. hominis was detected in significantly fewer samples, i.e. only from 9 (8.1%) samples. From these, 6 (5.4%) women were positive for both microorganisms. The fertile group consisted from 23 women. The presence of U. urealyticum was detected in 8 (34.7%) of them. M. hominis was detected only in the mixture with U. urealyticum in 3 (13.0%) cases. The most effective antibiotic against both species in our study was doxycycline. Conclusion: The results show slightly higher incidence of M. hominis and U. urealyticum in the genitourinary tract of women with fertility problems compare with control group. The potential negative effect of these species on the reproduction ability of women was not observed.
- MeSH
- Anti-Bacterial Agents therapeutic use MeSH
- Reproductive Techniques, Assisted MeSH
- Azithromycin therapeutic use MeSH
- Ciprofloxacin therapeutic use MeSH
- DNA analysis MeSH
- Doxycycline therapeutic use MeSH
- Erythromycin therapeutic use MeSH
- Humans MeSH
- Mycoplasma hominis * pathogenicity drug effects MeSH
- Polymerase Chain Reaction methods MeSH
- Prevalence MeSH
- Ureaplasma urealyticum pathogenicity drug effects MeSH
- Infertility, Female * complications therapy MeSH
- Check Tag
- Humans MeSH
- Female MeSH
- MeSH
- Vaginosis, Bacterial microbiology MeSH
- Chlamydia Infections epidemiology MeSH
- Humans MeSH
- Mycoplasma Infections epidemiology MeSH
- Uterine Cervical Diseases microbiology MeSH
- Sexually Transmitted Diseases, Bacterial MeSH
- Ureaplasma Infections epidemiology MeSH
- Infertility, Female microbiology MeSH
- Check Tag
- Humans MeSH
- Female MeSH
Článek se zabývá mykoplazmatickými infekcemi urogenitálního traktu. Shrnuje definici, morfologii, biochemické vlastnosti, patogenetické aspekty, jakož i klinický obraz, komplikace, konsekvence, diagnostiku a doporučenou terapii urogenitálních mykoplazmatických infekcí.
Mycoplasma and Ureaplasma infections of genitourinary tract are reviewed. Definiton, pathogen morphology, biochemial properties,pathogenesis as well as clinical presentation, diagnostical tools and recommended management of such infections aresummarized.
- MeSH
- Anti-Bacterial Agents therapeutic use MeSH
- Doxycycline therapeutic use MeSH
- Virulence Factors MeSH
- Urinary Tract Infections diagnosis etiology drug therapy MeSH
- Reproductive Tract Infections * diagnosis etiology drug therapy MeSH
- Clinical Laboratory Techniques MeSH
- Humans MeSH
- Macrolides therapeutic use MeSH
- Infertility, Male etiology MeSH
- Mycoplasma genitalium isolation & purification pathogenicity MeSH
- Mycoplasma cytology isolation & purification pathogenicity MeSH
- Mycoplasma Infections * diagnosis drug therapy physiopathology MeSH
- Tetracycline therapeutic use MeSH
- Ureaplasma cytology isolation & purification pathogenicity MeSH
- Ureaplasma Infections * diagnosis drug therapy physiopathology MeSH
- Urethritis diagnosis etiology drug therapy MeSH
- Infertility, Female etiology MeSH
- Check Tag
- Humans MeSH
- Male MeSH
- Female MeSH
- Publication type
- Review MeSH
OBJECTIVE: This study aimed to evaluate the amniotic fluid protein profiles and the intensity of intraamniotic inflammatory response to Ureaplasma spp. and other bacteria, using the multiplex xMAP technology. METHODS: A retrospective cohort study was undertaken in the Department of Obstetrics and Gynecology, University Hospital Hradec Kralove, Czech Republic. A total of 145 pregnant women with preterm prelabor rupture of membranes between gestational age 24+0 and 36+6 weeks were included in the study. Amniocenteses were performed. The presence of Ureaplasma spp. and other bacteria was evaluated using 16S rRNA gene sequencing. The levels of specific proteins were determined using multiplex xMAP technology. RESULTS: The presence of Ureaplasma spp. and other bacteria in the amniotic fluid was associated with increased levels of interleukin (IL)-6, IL-8, IL-10, brain-derived neurotropic factor, granulocyte macrophage colony stimulating factor, monocyte chemotactic protein-1, macrophage inflammatory protein-1, and matrix metalloproteinasis-9. Ureaplasma spp. were also associated with increased levels of neurotropin-3 and triggering receptor expressed on myeloid cells-1. CONCLUSIONS: The presence of Ureaplasma spp. in the amniotic fluid is associated with a slightly different protein profile of inflammatory response, but the intensity of inflammatory response to Ureaplasma spp. is comparable with the inflammatory response to other bacteria.
- MeSH
- Amnion immunology microbiology MeSH
- Pregnancy Complications, Infectious diagnosis immunology MeSH
- Cohort Studies MeSH
- Humans MeSH
- Young Adult MeSH
- Infant, Newborn MeSH
- Amniotic Fluid immunology microbiology MeSH
- Proteins analysis immunology MeSH
- Pregnancy MeSH
- Ureaplasma immunology isolation & purification MeSH
- Ureaplasma Infections diagnosis immunology MeSH
- Inflammation immunology microbiology MeSH
- Check Tag
- Humans MeSH
- Young Adult MeSH
- Infant, Newborn MeSH
- Pregnancy MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
OBJECTIVE: To evaluate Ureaplasma species and Mycoplasma hominis DNA in the cervical fluid and their association with microbial invasion of the amniotic cavity (MIAC) and/or histological chorioamnionitis (HCA) in pregnancies complicated by preterm prelabor rupture of membranes (PPROM). STUDY DESIGN: A prospective study of 68 women with singleton pregnancies complicated by PPROM between 24(0/7) and 36(6/7) weeks was conducted. Cervical fluid and amniotic fluid were collected from all women at the time of admission. The Ureaplasma species and Mycoplasma hominis DNA in the cervical fluid were identified using specific real-time PCR. RESULTS: Ureaplasma species and Mycoplasma hominis DNA were identified in 59% (40/69) of the cervical fluid samples. Women with the presence of Ureaplasma species DNA with and without Mycoplasma hominis DNA in the cervical fluid had a higher rate of MIAC alone [35% (14/40) versus 11% (3/28); p = 0.02] and a higher rate of the presence of both MIAC and HCA [30% (12/40) versus 4% (1/28); p = 0.01] than women without Ureaplasma species and Mycoplasma hominis DNA in the cervical fluid. CONCLUSIONS: The presence of Ureaplasma species DNA with and without Mycoplasma hominis DNA in the cervical fluid is associated with a higher risk of MIAC or MIAC and HCA together in pregnancies complicated by PPROM.
- MeSH
- Chorioamnionitis microbiology MeSH
- Adult MeSH
- Humans MeSH
- Young Adult MeSH
- Mycoplasma hominis isolation & purification MeSH
- Amniotic Fluid microbiology MeSH
- Fetal Membranes, Premature Rupture microbiology MeSH
- Pregnancy MeSH
- Ureaplasma isolation & purification MeSH
- Check Tag
- Adult MeSH
- Humans MeSH
- Young Adult MeSH
- Pregnancy MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
