The aim of this study was to develop the potential tissue engineering applications of d-glucosamine (GlcN) immobilized onto the surface of a biodegradable matrix in order to induce a desired biological effect at biointerfaces. Thus, for sample preparation we used a novel multistep physicochemical approach. In the first step the poly(lactic acid) (PLA) films were exposed to a low pressure plasma in air atmosphere, followed by radical graft copolymerization with acrylic acid to yield a carboxyl-functionalized spacer layer on the PLA surface. The carboxyl groups were then coupled to GlcN molecules via the carbodiimide chemistry. The developed surfaces were characterized by X-ray Photoelectron Spectroscopy (XPS), Contact angle measurements and Atomic Force Microscopy (AFM). A preliminary study on the proliferation of fibroblasts on the developed surfaces was performed using the NIH/3T3 cell line.
- MeSH
- Biocompatible Materials chemistry MeSH
- Photoelectron Spectroscopy MeSH
- Glucosamine chemistry MeSH
- Microscopy, Atomic Force MeSH
- Polyesters chemistry MeSH
- Surface Properties MeSH
- Cell Proliferation MeSH
- Regeneration * MeSH
- Wettability MeSH
- Tissue Engineering * MeSH
- Publication type
- Journal Article MeSH
Synthesis of theranostic nanoparticles, which combine both therapeutic and diagnostic capabilities in one platform can be considered as a step forward personalized medicine, since it allows tracing the delivery of the drug to targeted organ. Thus, the aim of this work was to prepare gadolinium alginate gel nanoparticles (gadolinum nanogels - GdNG) by the reverse microemulsions and physical crosslinking method as the vehicles able to carry hydrophilic drugs and to be traced by the Magnetic Resonance Imaging (MRI). The average size of synthesized nanoparticles was about 110nm and the batch concentration was 10(10) particles/ml. The morphology of nanogeles was visualized by Cryo-Scanning Electron Microscopy. Surface of nanogels particles was modified by the Layer-by-Layer (LbL) technique using natural polyelectrolytes. The cytotoxicity of non-modified and LbL modified nanogels was evaluated by the cellular viability quantification and cell death assessments using MTT and LDH biochemical tests, respectively. We encapsulated the model compound - fluorescent dye (Rhodamine b) in nanogels networks and proved the possibility of GdNG visualization by MRI.
- MeSH
- Alginates chemistry MeSH
- Cell Death MeSH
- Fluorescent Dyes chemistry MeSH
- Gadolinium chemistry MeSH
- Humans MeSH
- Magnetic Resonance Imaging MeSH
- Cell Line, Tumor MeSH
- Polyethylene Glycols chemical synthesis chemistry MeSH
- Polyethyleneimine chemical synthesis chemistry MeSH
- Theranostic Nanomedicine * MeSH
- Cell Survival MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
The growing number of people suffering from civilization diseases increases the amount of medication taken. Thus, novel methods for drug delivery must be developed which will constitute an alternative to oral administration. A new hope for patients bring transdermal drug delivery systems. To overcome skin barrier function, they must be prepared from materials which increase cell membrane permeability for the medication. Therefore, there is an increasing need for novel, advanced transdermal systems capable of controlled active substance release under specific stimuli. The aim of this research was to obtain novel hydrogel-based transdermal delivery systems through crosslinking process of chitosan using azelaic acid followed by doping with ZnO nanorods to enhance its drug sorption properties. Ready materials were investigated over their structure, morphology and durability. Drug loading capacity, controlled drug release ability and its kinetics were determined on medication used in treatment of cardiovascular system diseases - acetylsalicylic acid. Finally, lack of cytotoxicity was confirmed by XTT assay and cell morphology study carried out on L929 mouse fibroblasts. Obtained results show a great potential of the developed transdermal delivery systems in active substances administration through skin tissue and may help to protect digestive tract of the patients in the future.
- MeSH
- Administration, Cutaneous MeSH
- Chitosan * MeSH
- Hydrogels MeSH
- Dicarboxylic Acids MeSH
- Drug Delivery Systems MeSH
- Humans MeSH
- Mice MeSH
- Zinc Oxide * MeSH
- Drug Liberation MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
Polymers with functionalized surfaces have attracted a lot of attention in the last few years. Due to the progress in the techniques of polymer micro-patterning, miniaturized bioanalytical assays and biocompatible devices can be developed. In the presented work, we performed surface modification of polyethylene naphthalate (PEN) foil by an excimer laser beam through a photolithographic contact mask. The aim was to fabricate micro-patterned areas with surface functional groups available for localized covalent immobilization of biotin. It was found out that depending on the properties of the laser scans, a polymer surface exhibits different degrees of modification and as a consequence, different degrees of surface biotinylation can be achieved. Several affinity tests with optical detection of fluorescently labeled streptavidin were successfully performed on biotinylated micro-patterns of a PEN foil. The polymer surface properties were also evaluated by electrokinetic analysis, Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). The results have shown that PEN foils can be considered suitable substrates for construction of micro-patterned bioanalytical affinity assays.
- MeSH
- Biotin chemistry MeSH
- Biotinylation MeSH
- Photochemical Processes MeSH
- Lab-On-A-Chip Devices MeSH
- Lasers, Excimer MeSH
- Microtechnology MeSH
- Naphthalenes chemistry radiation effects MeSH
- Polyethylenes chemistry radiation effects MeSH
- Surface Properties MeSH
- Streptavidin chemistry MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Herein, we report a novel concept of low-cost flexible platform for fluorescence-based biosensor. The surface of polyethylene naphthalate (PEN) foil was exposed to KrF excimer laser through a photolitographic contact mask. Laser initiated surface modification resulted in micro-patterned areas with surface functional groups available for localized covalent immobilization of biotin. High affinity binding protein (albumin-binding domain (ABD) of protein G, Streptococcus G148) recognizing human serum albumin (HSA), genetically fused with streptavidin (SA-ABDwt), was immobilized on the micro-patterned surface through biotin-streptavidin coupling. Fluorescently labelled HSA analyte was detected in several blocking environments, in 1% bovine serum albumin (BSA) and 6% fetal serum albumin (FBS), respectively. We conclude that the presented novel concept enabled us to micropattern functional biosensing layers on the surface of PEN foil in a fast and easy way. It brings all necessary aspects for continuous roll-to-roll fabrication of low-cost optical bioanalytical devices.
- MeSH
- Biotin metabolism MeSH
- Photoelectron Spectroscopy MeSH
- Humans MeSH
- Microtechnology methods MeSH
- Naphthalenes chemistry MeSH
- Optical Phenomena * MeSH
- Polyethylenes chemistry MeSH
- Surface Properties MeSH
- Serum Albumin metabolism MeSH
- Streptavidin metabolism MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
To employ dual advantages of emulsion and gel, a facile approach was investigated to fabricate core/shells structured hydrogel beads based on sodium alginate (SA) via Pickering emulsion template and in situ gelation. The encapsulation and controlled release behavior were further studied using lysozyme (Ly) as the model protein. The optical micrographs and SEM images indicated the SA beads could well disperse with the size about 150 μm. CaCO3 microparticles were strong adhesive onto SA gel. It showed that 96.51 ± 0.62% Ly was loaded into the hydrogel beads. The released behavior of Ly could be regulated by external pH condition, and displayed highest release rate at pH 5.0. Whereas the lowest release rate was recorded at pH 7.0. The released behavior well followed the Hixcon-Crowell model which indicated that the release mechanism of Ly followed the corrosion diffusion law. The worth-while endeavor provide an artful and facile approach using Pickering emulsion template and in situ gelation to fabricate core/shells structured SA beads with high load capacity and controlled regulation of the entrapped functional component.
- MeSH
- Alginates chemistry MeSH
- Diffusion MeSH
- Emulsions MeSH
- Hydrogels chemistry MeSH
- Kinetics MeSH
- Hydrogen-Ion Concentration MeSH
- Delayed-Action Preparations * MeSH
- Muramidase chemistry MeSH
- Drug Compounding methods MeSH
- Solutions MeSH
- Calcium Carbonate chemistry MeSH
- Drug Liberation MeSH
- Phase Transition MeSH
- Publication type
- Journal Article MeSH
Magnetic harvesting of microalgal biomass provides an attractive alternative to conventional methods. The approach to this issue has so far been pragmatic, focused mainly on finding cheap magnetic agents in combination with harvestable microalgae species. The aim of this work was to study experimentally and theoretically the mechanisms leading to cell-magnetic agent attachment/detachment using real experiments and predictions made by colloidal adhesion (XDLVO) model. Two types of well defined magnetic beads (MBs) carrying ion exchange functional groups (DEAE - diethylaminoethyl and PEI - polyethylenimine) were studied in connection with microalgae (Chlorella vulgaris). Optimal harvesting efficiencies (>90%) were found for DEAE and PEI MBs, while efficient detachment was achieved only for DEAE MBs (>90%). These findings were in accordance with the predictions by XDLVO model. Simultaneously there was found a discrepancy between the XDLVO prediction and the poor detachment of PEI MBs from microalgal surface. This can be ascribed to an additional interaction (probably covalent bonds) between PEI and algal surface, which the XDLVO model is unable to capture given by its non-covalent nature.
- MeSH
- Models, Biological MeSH
- Biomass MeSH
- Cell Adhesion MeSH
- Chlorella vulgaris isolation & purification physiology MeSH
- Ethanolamines chemistry MeSH
- Ion Exchange MeSH
- Colloids MeSH
- Magnetic Phenomena MeSH
- Magnetite Nanoparticles chemistry MeSH
- Microalgae isolation & purification physiology MeSH
- Polyethyleneimine chemistry MeSH
- Surface Properties MeSH
- Industrial Microbiology methods MeSH
- Fresh Water microbiology MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Polyaniline is a promising conducting polymer with still increasing application potential in biomedicine. Its surface modification can be an efficient way how to introduce desired functional groups and to control its properties while keeping the bulk characteristics of the material unchanged. The purpose of the study was to synthetize thin films of pristine conducting polyaniline hydrochloride, non-conducting polyaniline base and polyaniline modified with poly(2-acrylamido-2-methyl-1-propanesulfonic acid) (PAMPSA) and investigate chosen parameters of their hemocompatibility. The modification was performed either by introduction of PAMPSA during the synthesis or by reprotonation of polyaniline base. The polyaniline hydrochloride and polyaniline base had no impact on blood coagulation and platelet adhesion. By contrast, the polyaniline reprotonated with PAMPSA completely hindered coagulation thanks to its interaction with coagulation factors Xa, Va and IIa. The significantly lower platelets adhesion was also found on this surface. Moreover, this film maintains its conductivity at pH of 6, which is an improvement in comparison with standard polyaniline hydrochloride losing most of its conductivity at pH of 4. Polyaniline film with PAMPSA introduced during synthesis had an impact on platelet adhesion but not on coagulation. The combined conductivity, anticoagulation activity, low platelet adhesion and improved conductivity at pH closer to physiological, open up new possibilities for application of polyaniline reprotonated by PAMPSA in blood-contacting devices, such as catheters or blood vessel grafts.
A plasmonic biosensor for rapid detection of protein biomarkers in complex media is reported. Clinical serum samples were analyzed by using a novel biointerface architecture based on poly[(N-(2-hydroxypropyl) methacrylamide)-co-(carboxybetaine methacrylamide)] brushes functionalized with bioreceptors. This biointerface provided an excellent resistance to fouling even after the functionalization and allowed for the first time the direct detection of antibodies against hepatitis B surface antigen (anti-HBs) in clinical serum samples using surface plasmon resonance (SPR). The fabricated SPR biosensor allowed discrimination of anti-HBs positive and negative clinical samples in 10min. Results are validated by enzyme-linked immunoassays of the sera in a certified laboratory. The sensor could be regenerated by simple treatment with glycine buffer.
- MeSH
- Acrylamides chemistry MeSH
- Equipment Design MeSH
- Hepatitis B Surface Antigens immunology MeSH
- Hepatitis B blood immunology MeSH
- Humans MeSH
- Limit of Detection MeSH
- Surface Plasmon Resonance instrumentation MeSH
- Surface Properties MeSH
- Antibodies, Viral blood immunology MeSH
- Hepatitis B virus immunology isolation & purification MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Validation Study MeSH
Adsorption of HIV protease onto surfaces that are usually considered to be protein-resistant was studied quantitatively using surface plasmon resonance. Adsorption onto gold surfaces functionalized by OH-terminated alkyl chains was much stronger than onto oligo(ethylene glycol)-terminated surfaces. Equilibrium and kinetic adsorption constants were determined. An anomalous mutual attraction between adsorbate molecules was observed, indicating the possibility of two-dimensional crystallization of HIV protease. These results are applicable for the design of sensors/biosensors for HIV protease resistance detection and for proper manipulation of this enzyme in laboratory devices.