The endogenous auxin and cytokinin contents of in vitro regenerated Tulbaghia simmleri maintained on applied plant growth regulators in Murashige and Skoog (MS) medium were investigated using UHPLC-MS analysis. The highest number of shoots (27.6 per leaf) were produced in MS medium supplemented with 2.5 μM thidiazuron. A higher number of these shoots were rooted with 10 μM 6-(2-hydroxy-3-methylbenzylamino) purine (PI-55, cytokinin antagonist). Production of somatic embryos (SEs: 16.4-4.6, globular to cotyledonary stages) improved significantly with liquid MS medium containing 2.5 μM picloram, 2.5 μM phloroglucinol (PG) and 1.5 μM gibberellic acid or 1.5 μM PI-55 and 1.0 μM trans-zeatin. SEs (torpedo and cotyledonary stages) germinated (100%) in plant growth regulator free MS medium. The plantlets were acclimatized and all survived in the greenhouse. Higher levels of endogenous auxin, 2-oxindole-3-acetic acid (oxIAA, 371.52 pmol/g DW) and indole-3-acetylaspartate (IAAsp, 141.56 pmol/g DW) were detected in shoots from PG treatments. The roots of garden-grown mother plants possessed the highest level of indole-3-acetic acid (IAA, 630.54 pmol/g DW) and oxIAA (515.26 pmol/g DW). Cytokinins [CKs: trans-zeatin-O-glucoside (tZOG), cis-zeatin (cZ) and N6-isopentenyladenosine-5'-monophosphate (iPRMP)] levels were relatively high in shoots and roots of plantlets in vitro. However, PI-55 treatments influenced the development of plantlets promoting a higher biosynthesis level of iPRMP (418.06 pmol/g DW in root) and cZRMP (904.61 pmol/g DW in roots and 1427.83 pmol/g DW in shoots). The presented protocols offer organogenesis and somatic organogenesis systems for rapid plant regeneration of T. simmleri. In addition, the importance of exogenous and endogenous hormonal effects on in vitro plant growth and development as well as endogenous hormone metabolism signalling and transport related to the physiological processes of CK metabolism and transport are illustrated for in vitro development of T. simmleri.

• MeSH
• Allium fyziologie   MeSH
• botanika metody   MeSH
• regenerace * MeSH
• regulátory růstu rostlin metabolismus   MeSH
• techniky in vitro metody   MeSH
• Publikační typ
• časopisecké články MeSH

• MeSH
• biflavonoidy MeSH
• fenoly farmakologie   MeSH
• floroglucinol farmakologie   MeSH
• regulátory růstu rostlin MeSH
• rostliny MeSH
• vývoj rostlin MeSH

• MeSH
• ekotoxikologie MeSH
• infertilita MeSH
• rozmnožování MeSH
• techniky in vitro MeSH
• testy toxicity MeSH
• vystavení vlivu životního prostředí MeSH
• Publikační typ
• kongresy MeSH
• sborníky MeSH
• zprávy MeSH

• Konspekt
• Farmacie. Farmakologie
• NLK Obory
• toxikologie
• reprodukční lékařství

• MeSH
• biomedicínský výzkum MeSH
• experimentální terapie MeSH
• intravitální mikroskopie MeSH
• metastázy nádorů MeSH
• techniky in vitro MeSH
• Publikační typ
• monografie MeSH

• Konspekt
• Patologie. Klinická medicína
• NLK Obory
• onkologie
• experimentální medicína

• MeSH
• buněčná diferenciace MeSH
• buňky kostní dřeně MeSH
• hematopoetické kmenové buňky MeSH
• hydrokortison nedostatek   MeSH
• kultivační média MeSH
• kultivované buňky MeSH
• počet buněk MeSH
• techniky in vitro MeSH

• MeSH
• embryo savčí fyziologie   MeSH
• fertilizace in vitro veterinární   MeSH
• přežvýkavci embryologie   genetika   MeSH
• skot MeSH
• translokace genetická MeSH
• vývoj plodu MeSH
• zvířata MeSH
• Check Tag
• skot MeSH
• zvířata MeSH

• MeSH
• acetylcholin MeSH
• adrenalin MeSH
• embryo savčí MeSH
• embryonální vývoj MeSH
• krysa rodu rattus MeSH
• nervový přenos MeSH
• novorozená zvířata růst a vývoj   MeSH
• plod MeSH
• srdeční frekvence MeSH
• techniky in vitro MeSH
• vývoj plodu MeSH
• Check Tag
• krysa rodu rattus MeSH

• MeSH
• chrupavka chemie   MeSH
• kosti a kostní tkáň MeSH
• minerály MeSH
• osteogeneze MeSH
• techniky in vitro MeSH
• vývoj kostí MeSH
• želatina MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH

In this article, we focused on the impact of precisely chemically modified FLI maturation medium enriched with fibroblast growth factor 2 (FGF2), leukemia inhibitory factor (LIF), insulin-like growth factor 1 (IGF1), and polyvinyl alcohol (PVA) and its potential to improve the efficiency of in vitro production of porcine embryos. We hypothesized that enhancing the composition of the maturation medium could result in an elevated production of embryos in vitro and can affect EGA. FLI medium resulted in a significantly higher rate of oocyte blastocyst maturation and formation compared to the control DMEM medium. In addition, immunocytochemical labelling confirmed the detection of UBF in 4-cell FLI parthenogenic embryos, suggesting similarities with natural embryo development. Through RNAseq analysis, upregulated genes present in 4-cell FLI embryos were found to play key roles in important biological processes such as cell proliferation, cell differentiation, and transcriptional regulation. Based on our findings, we demonstrated the positive influence of FLI medium in the evaluation of in vitro embryo production, EGA detection, transcriptomic and proteomic profile, which was confirmed by the positive activation of the embryonal genome in the 4-cell stage of parthenogenetically activated embryos.

• MeSH
• blastocysta účinky léků   metabolismus   MeSH
• fertilizace in vitro MeSH
• fibroblastový růstový faktor 2 * farmakologie   MeSH
• insulinu podobný růstový faktor I * farmakologie   MeSH
• kultivační média * chemie   farmakologie   MeSH
• leukemický inhibiční faktor * farmakologie   MeSH
• oocyty MeSH
• prasata embryologie   genetika   MeSH
• proteomika MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH

• MeSH
• buněčná imunita MeSH
• růst a vývoj MeSH
• techniky in vitro MeSH
• vývojová biologie MeSH