surface plasmons Dotaz Zobrazit nápovědu
Fundamentals of surface plasmon resonance (SPR) sensors 5 -- 3. Present state of the art 6 -- 3.1. Surface plasmon resonance method 10 -- 5.1. Theory of SPR sensors 10 -- 5.2. Multi-surface-plasmon spectroscopy for information-rich biosensing 13 -- 5.4. Long-range surface plasmons for high-performance SPR sensors 14 -- 5.5. SPRCD approach to spectroscopy of surface plasmons 15 -- 6.
32 s. : il. ; 21 cm
- MeSH
- biofyzika MeSH
- biosenzitivní techniky MeSH
- mikrochemie MeSH
- optické jevy MeSH
- povrchová plasmonová rezonance MeSH
- Publikační typ
- vysokoškolské kvalifikační práce MeSH
- Konspekt
- Biochemie. Molekulární biologie. Biofyzika
- NLK Obory
- fyzika, biofyzika
We report a new approach to characterization of thin (bio)molecular films based on spectroscopy of Bragg-scattered surface plasmons (BSSPs) generated by diffraction-coupling of counterpropagating surface plasmons on a metal-coated diffraction grating. The BSSPs exhibit fields with different penetration depths into the medium adjacent to the metal and therefore exhibit unequal sensitivities to the presence of (bio)molecular films on the surface of the metal. Therefore, spectroscopy of BSSPs enables in situ observation of the formation of biomolecular films and determination of both their refractive index and thickness. We demonstrate this capacity of spectroscopy of BSSPs in a model experiment in which growth of protein layers on a gold surface is studied.
Cell volume and its regulation are key factors for cellular integrity and also serve as indicators of various cell pathologies. SPR sensors represent an efficient tool for real-time and label-free observations of changes in cell volume and shape. Here, we extend this concept by employing the use of long-range surface plasmons (LRSP). Due to the enhanced penetration depth of LRSP (~1μm, compared to ~0.4μm of a conventional surface plasmon), the observation of refractive index changes occurring deeper inside the cells is possible. In this work, the responses of a confluent normal rat kidney (NRK) epithelial cell layer to osmotic stress are studied by both conventional and long-range surface plasmons. Experiments are conducted in parallel using cell layers grown and stimulated under the same conditions to enable direct comparison of the results and discrimination of the osmotic stress-induced effects in different parts of the cell.
- MeSH
- analýza selhání vybavení MeSH
- barvení a značení MeSH
- biosenzitivní techniky přístrojové vybavení MeSH
- buněčné linie MeSH
- design vybavení MeSH
- krysa rodu rattus MeSH
- ledviny cytologie fyziologie MeSH
- osmotický tlak MeSH
- počítačové systémy MeSH
- povrchová plasmonová rezonance přístrojové vybavení MeSH
- reprodukovatelnost výsledků MeSH
- senzitivita a specificita MeSH
- velikost buňky MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Surface plasmon resonance (SPR) biosensors are affinity sensing devices exploiting a special mode of electromagnetic field-surface plasmon-polariton-to detect the binding of analyte molecules from a liquid sample to biomolecular recognition elements immobilized on the surface of the sensor. In this paper, we review advances of SPR biosensor technology towards detection systems for the simultaneous detection of multiple analytes (multi-analyte detection). In addition, we report application of a recently developed multichannel SPR sensor based on spectroscopy of surface plasmons and wavelength division multiplexing of sensing channels to multi-analyte detection.
Lectin biochips and biosensors are used to detect and study the protein glycosylation. Glycosylation changes are accompanied by changes in physiological state, which may be associated with certain types of diseases such as cancer, rheumatoid arthritis, multiple sclerosis, etc. In recent years, this issue has been attracting more and more scientists and enormous advances have been achieved in this field. This work is focused on the use of surface plasmon resonance (SPR) in combination with lectin biosensors and biochips enabling tracking glycosylation and its changes. SPR is commonly used to detect proteins and to study the protein-protein and protein-drug interactions. Lectin SPR biochips additionally allow us to detect the glycan (glycoprotein)-lectin (protein) interactions. The great advantage of SPR, as compared to most other methods used for this purpose, is the possibility of real-time and label-free measurements. On the other hand, the measurement of large number of samples is time consuming. This is possible to overcome by using the SPR imaging (SPRi) techniques allowing simultaneous measurement of several samples. Practical applications of the lectin SPR biosensors and biochips are not only in biology and biomedicine research and diagnosis of diseases and detection of pathogenic microorganisms, but also in environmental monitoring, food control and even in the military for the detection of substances based on glycoprotein toxins.
Surface plasmon resonance sensors have made vast advancements in the sensing technology and the number of applications achievable. New developments in surface plasmon resonance sensors have gained considerable momentum promoted by the urgent needs of fast, reliable and label-free methods for detection and quantification of analytes in molecular biology, medicine and other life sciences. However, even if enormous improvements in the limits of detections have been achieved, this technology still faces important challenges to be translated to clinical practice or in-field measurements. This paper reviews the important recent advances of this technology for the label-free detection in real biological samples and we discussed the key challenges to be overcome to transit from prototypes to commercial biosensors.
- MeSH
- biologie přístrojové vybavení metody MeSH
- diagnóza MeSH
- lidé MeSH
- povrchová plasmonová rezonance přístrojové vybavení metody MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- přehledy MeSH
We present a compact surface plasmon resonance (SPR) biosensor for the detection of bisphenol A (BpA), an endocrine-disrupting chemical. The biosensor is based on an SPR sensor platform (SPRCD) and the binding inhibition detection format. The detection of BpA in PBS and wastewater was performed at concentrations ranging from 0.05 to 1,000 ng/ml. The limit of detection for BpA in PBS and wastewater was estimated to be 0.08 and 0.14 ng/ml, respectively. It was also demonstrated that the biosensor can be regenerated for repeated use. Results achieved with the SPR biosensor are compared with those obtained using ELISA and HPLC methods.
Surface plasmon resonance microscopy and imaging are optical methods that enable observation and quantification of interactions of nano- and microscale objects near a metal surface in a temporally and spatially resolved manner. This review describes the principles of surface plasmon resonance microscopy and imaging and discusses recent advances in these methods, in particular, in optical platforms and functional coatings. In addition, the biological applications of these methods are reviewed. These include the detection of a broad variety of analytes (nucleic acids, proteins, bacteria), the investigation of biological systems (bacteria and cells), and biomolecular interactions (drug-receptor, protein-protein, protein-DNA, protein-cell).
- MeSH
- Bacteria izolace a purifikace ultrastruktura MeSH
- design vybavení MeSH
- lidé MeSH
- mapování interakce mezi proteiny přístrojové vybavení metody MeSH
- mikroskopie přístrojové vybavení metody MeSH
- nukleové kyseliny analýza MeSH
- povrchová plasmonová rezonance přístrojové vybavení metody MeSH
- proteiny analýza MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- přehledy MeSH
Bisphenol A (BpA) is a chemical that is extensively used in common plastic products, such as food and drink containers. It can leach from the plastics and penetrate into the human body, where it acts as an endocrine disruptor with significant risks to human health. In order to minimize the exposure of human populations to BpA, methods for the detection of BpA are needed. In this work, we present a novel surface plasmon resonance (SPR) biosensor for ultrasensitive detection of BpA. Our approach combines a binding inhibition assay with functionalized gold nanoparticles for the enhancement of sensor response. We demonstrate that the developed biosensor enables the detection of BpA in a wide range of concentrations (0.01 to 100,000 ng/mL) with an extremely low limit of detection-5.2 pg/mL.
Understanding the molecular mechanism of HIV-1 integrase (IN) activity is critical to find functional inhibitors for an effective AIDS therapy. A robust, fast, and sensitive method for studying IN activity is required. In this work, an assay for real-time label-free monitoring of the IN activity based on surface plasmon resonance was developed. This assay enabled direct monitoring of the integration of a viral doubled-stranded (ds) DNA into the host genome. The strand transfer reaction was detected by using two different DNA targets: supercoiled plasmid (pUC 19) and short palindrome oligonucleotide. The effect of the length of the DNA target on the possibility to monitor the actual process of the strand transfer reaction is discussed. The surface density of integrated ds-DNA was determined. IN binding to the oligonucleotide complexes and model DNA triplexes in the presence of various divalent ions as metal cofactors was investigated as well. The assay developed can serve as an important analytical tool to search for potential strand transfer reaction inhibitors as well as for the study of compounds interfering with the binding of ds long terminal repeats-IN complexes with the host DNA.
