- MeSH
- analýza potravin * MeSH
- anthokyaniny MeSH
- chléb analýza MeSH
- jedlá semena MeSH
- karotenoidy MeSH
- lidé MeSH
- mouka MeSH
- nutriční hodnota * MeSH
- potravní vláknina MeSH
- proteiny MeSH
- pšenice MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- práce podpořená grantem MeSH
Bread wheat (Triticum aestivum L.) is one of the most valuable cereal crops for human consumption. Its grain storage proteins define bread quality, though they may cause food intolerances or allergies in susceptible individuals. Herein, we discovered a diversity of grain proteins in three Ukrainian wheat cultivars: Sotnytsia, Panna (both modern selection), and Ukrainka (landrace). Firstly, proteins were isolated with a detergent-containing buffer that allowed extraction of various groups of storage proteins (glutenins, gliadins, globulins, and albumins); secondly, the proteome was profiled by the two-dimensional gel electrophoresis. Using multi-enzymatic digestion, we identified 49 differentially accumulated proteins. Parallel ultrahigh-performance liquid chromatography separation followed by direct mass spectrometry quantification complemented the results. Principal component analysis confirmed that differences among genotypes were a major source of variation. Non-gluten fraction better discriminated bread wheat cultivars. Various accumulation of clinically relevant plant proteins highlighted one of the modern genotypes as a promising donor for the breeding of hypoallergenic cereals.
- MeSH
- 2D gelová elektroforéza MeSH
- albuminy chemie genetika metabolismus MeSH
- chléb analýza MeSH
- gliadin chemie genetika MeSH
- globuliny chemie genetika MeSH
- gluteny chemie genetika MeSH
- jedlá semena chemie genetika MeSH
- lidé MeSH
- obilninové proteiny chemie klasifikace MeSH
- proteom genetika MeSH
- pšenice chemie genetika MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
KEY MESSAGE: Using COS markers, the study reveals homeologous relationships between tetraploid Agropyron cristatum and bread wheat to support alien introgression breeding of wheat. Crested wheatgrass (Agropyron cristatum L. Gaertn.) is a wild relative of wheat that possesses many genes that are potentially useful in wheat improvement. The species comprises a complex of diploid, tetraploid and hexaploid forms. In this study, wheat-A. cristatum chromosome, telosome and translocation lines were used to characterize syntenic relationships between tetraploid A. cristatum and bread wheat. Prior to mapping COS markers, the cytogenetic stock lines were characterized for fertility and by FISH and GISH for karyotype stability. Out of 328 COS markers selected for the study, 279 consistently amplified products in tetraploid A. cristatum, and, out of these, 139 were polymorphic between tetraploid crested wheatgrass and wheat. Sixty-nine markers were found to be suitable for the detection of tetraploid A. cristatum chromosomes 1P-6P in wheat, ranging from 6 to 17 markers per chromosome. BLASTn of the source ESTs resulted in significant hits for 67 markers on the wheat pseudomolecules. Generally, COS markers of the same homeologous group were detected on similar arms in both Agropyron and wheat. However, some intragenomic duplications and chromosome rearrangements were detected in tetraploid A. cristatum. These results provide new insights into the structure and evolution of the tetraploid A. cristatum genome and will facilitate the exploitation of the wild species for introgression breeding of bread wheat.
- MeSH
- Agropyron genetika růst a vývoj MeSH
- chléb analýza MeSH
- chromozomy rostlin MeSH
- genetické markery * MeSH
- genom rostlinný genetika MeSH
- genotyp MeSH
- hybridizace genetická * MeSH
- mapování chromozomů MeSH
- pšenice genetika růst a vývoj MeSH
- tetraploidie * MeSH
- translokace genetická MeSH
- Publikační typ
- časopisecké články MeSH
- Klíčová slova
- cereální výrobky, pekařské výrobky, cukrářské výrobky, trvanlivé pečivo, pečivo,
- MeSH
- chléb * analýza klasifikace MeSH
- jedlá semena klasifikace MeSH
- manipulace s potravinami MeSH
- mouka analýza klasifikace MeSH
- potravinářská technologie metody MeSH
- průmysl zpracování potravin MeSH
- stravovací služby * MeSH
The anthocyanin composition of blue (Triticum aestivum L., cv. Skorpion) and purple wheat (Triticum aethiopicum JAKUBZ cv. Abyssinskaja arrasajta cv. Abyssinskaja arrasajta), cultivated in the Czech Republic, and of the prepared whole blue and purple wheat bread was determined. In blue and purple wheat, 19 and 26 anthocyanins, respectively, were tentatively identified by liquid chromatography and mass spectrometry. The total content of anthocyanins determined in blue and purple wheat was 9.26 and 13.23 mgkg(-1), respectively. The breads were baked at 240 and 180 °C. Some significant differences in anthocyanins content were observed between breads prepared at different baking temperatures. The content of cyanidin-3-glucoside, delphinidin-3-glucoside and pelargonidin-3-glucoside was determinated in starting material, whole meal flours and baked breads. These kinds of wheat are suitable for baking bread, since intake of anthocyanins may play an important role in the prevention of human diseases.
- MeSH
- anthokyaniny analýza chemie MeSH
- chléb analýza MeSH
- chromatografie kapalinová MeSH
- druhová specificita MeSH
- glukosidy analýza MeSH
- hmotnostní spektrometrie s elektrosprejovou ionizací MeSH
- jedlá semena chemie MeSH
- lidé MeSH
- mouka analýza MeSH
- pšenice chemie MeSH
- vaření * MeSH
- vysoká teplota * MeSH
- vysokoúčinná kapalinová chromatografie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
- Klíčová slova
- domácí pečení chleba,
- MeSH
- bezlepková dieta * využití MeSH
- celiakie * diagnóza dietoterapie prevence a kontrola MeSH
- chléb * analýza klasifikace normy využití zásobování a distribuce MeSH
- jedlá semena MeSH
- lidé MeSH
- lipidy MeSH
- minerály MeSH
- mouka MeSH
- nutriční hodnota MeSH
- potravinářské přísady MeSH
- potravinářský průmysl MeSH
- proteiny MeSH
- sacharidy MeSH
- Check Tag
- lidé MeSH
