OBJECTIVE: To establish the effect of chlorinated organic compounds on fertility. DESIGN: We analyzed the influence of polychlorinated biphenyls (PCBs) and pesticides in follicular fluid on the number of fertilized oocytes, the quality of oocytes and embryos, pregnancy rate, and number of embryos cryoconserved in IVF-ET program. SETTING: Department of Gynecology and Obstetrics, Charles University, First Faculty of Medicine and General Teaching Hospital, Prague, Czech Republic. PATIENT(S): Patients from the Center of Assisted Reproduction. INTERVENTION(S): We collected the follicular fluid of 99 infertile women. MAIN OUTCOME MEASURE(S): The levels of xenobiotics in follicular fluid were examined by high-resolution mass spectrometry and gas chromatography. Correlations between the levels of xenobiotics and IVF-ET program results were statistically analyzed. RESULT(S): The PCB levels varied from 0.1 to 254.6 ng/g of fat. Levels of DDT and its metabolites varied from 0.3 to 35 228.8 ng/g of fat. A correlation was found between the number of diploid oocytes and the level of DDT, the fertilization of oocytes and level of PCB 158, and the pregnancy rate and the number of embryos cryoconserved and levels of PCB 47. CONCLUSION(S): Xenobiotics can be detected in the fat compartment of the follicular fluid. Some correlation to the IVF-ET program results was established, to a degree that might be considered of concern, although it was not statistically significant at the 5% level.
- MeSH
- DDT pharmacology MeSH
- Adult MeSH
- Fertilization in Vitro methods MeSH
- Follicular Fluid drug effects physiology MeSH
- Environmental Pollutants pharmacology MeSH
- Humans MeSH
- Pesticides pharmacology MeSH
- Polychlorinated Biphenyls pharmacology MeSH
- Embryo Transfer methods MeSH
- Prognosis MeSH
- Pregnancy MeSH
- Pregnancy Rate MeSH
- Treatment Outcome MeSH
- Xenobiotics pharmacology MeSH
- Infertility, Female chemically induced diagnosis therapy MeSH
- Check Tag
- Adult MeSH
- Humans MeSH
- Pregnancy MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Clinical Trial MeSH
- Research Support, Non-U.S. Gov't MeSH
The aim of our study was to obtain data on the exposure of breast-fed infants to polychlorinated biphenyls (PCBs) and selected organochlorine pesticides (OCPs) in different urban areas of the Czech Republic. The PCB and OCP levels were determined in 90 human milk samples collected in seven urban areas in 1999-2000 according to the WHO protocol. The estimated daily intake (EDI) was calculated for each of the analytes and compared with the respective tolerable daily intake (TDI). Significant local differences in the sum of 35 PCB congeners analyzed (total PCBs) as well as in the most prominent indicator congeners 138, 153, and 180 values were observed, with the highest levels being found in breast milk samples from mothers living in the vicinity of a former plant using PCBs in Uherske Hradiste (median and ranges 3410 and 1448-13,754 ng g(-1)lipid weight (lw)). Non-exposed mothers from the same area had about threefold lower levels (median and ranges 1073 and 757-2139 ng/g lw, respectively). The lowest levels of total PCBs were found in Telc (median and ranges 480 and 293-731 ng g(-1)lw, respectively). In all study areas, EDIs for PCBs in breast-fed infants exceeded the TDI of 0.4 microg kg(-1)bwd(-1) recommended in the Czech Republic. EDI for HCB exceeded the recommended TDI of 0.16 microg kg(-1), EDI for DDT was slightly below TDI of 10 microg kg(-1) bw d(-1) and HCHs EDI was negligible. The database of analytical results from this study was used for Bayesian modeling of breast-fed infant exposure to PCBs and OCPs during the recommended 6 months of exclusive breastfeeding.
- MeSH
- Hydrocarbons, Chlorinated analysis chemistry MeSH
- Demography MeSH
- Adult MeSH
- Breast Feeding MeSH
- Humans MeSH
- Milk, Human chemistry MeSH
- Maternal Exposure MeSH
- Mothers MeSH
- Young Adult MeSH
- Environmental Monitoring MeSH
- Pesticides analysis chemistry MeSH
- Polychlorinated Biphenyls analysis chemistry MeSH
- Check Tag
- Adult MeSH
- Humans MeSH
- Young Adult MeSH
- Female MeSH
- Publication type
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Czech Republic MeSH
The aim of this study was to detect DDT and its metabolites, 1,1-dichloro-2,2-bis(4-chlorophenyl)ethene (DDE) and 1,1-dichloro-2,2-bis(4-chlorophenyl)ethane (DDD), in blood and follicular fluid of infertile women undergoing in vitro fertilization and the embryo transfer program. The accumulation of DDT, DDE and DDD in follicular fluid of 30 infertile women was confirmed. The concentrations of the compounds in blood were 1.7 ? 5985 in follicular fluid and 3.5 ? 35 229 ng g?1 fat, respectively.
Závěrečná zpráva o řešení grantu Interní grantové agentury MZ ČR
73 l. : il. ; 32 cm
Studium produktů teplotní degradace cizorodých látek obsažených v potravinách. XXX XXX XXX
- Conspectus
- Lékařské vědy. Lékařství
- NML Fields
- nutriční terapie, dietoterapie a výživa
- environmentální vědy
- NML Publication type
- závěrečné zprávy o řešení grantu IGA MZ ČR
Acta hygienica, epidemiologica et microbiologica, ISSN 0231-6544 Příl. č. 1/1993
50 s. : il., tab. ; 21 cm
- MeSH
- Biomarkers analysis MeSH
- Environmental Health classification MeSH
- Organic Chemicals analysis MeSH
- Gas Chromatography-Mass Spectrometry methods standards utilization MeSH
- Environmental Pollution analysis MeSH
- Publication type
- Guideline MeSH
- Conspectus
- Znečištění a poškození životního prostředí
- NML Fields
- environmentální vědy
- hygiena
