• Publikační typ
• abstrakt z konference MeSH

Lactobacillus, Bifidobacterium and Enterococcus cultures are increasingly used as probiotics for humans and pigs. The aim of this study was to investigate if co-cultivation of porcine and human neutrophils with probiotics can lead to increased apoptosis in vitro. Ten adult Large white pigs and 10 healthy human donors were used in this study. Neutrophils were isolated by dextran sedimentation and cultivated with and without the lactic acid bacteria Bifidobacterium bifidum, Lactobacillus rhamnosus, and Enterococcus faecium for 2, 4, 24 and 48 h. Early and late apoptosis was measured using flow cytometry, and cell lysis was detected based on lactate dehydrogenase activity (LDH). A significant (P < 0.05; P < 0.01) increase in apoptotic neutrophils and LDH was observed at 24 h and 48 h in vitro. All probiotics exerted their greatest effects on the early apoptosis of porcine neutrophils, while the effects of L. rhamnosus were most pronounced on late apoptosis and those of B. bifidumon LDH release of human neutrophils. The increased neutrophil apoptosis caused by probiotic bacteria can be beneficial for more efficient efferocytosis and faster resolution of inflammation and tissue regeneration. In conclusion, we have demonstrated that the interaction of B. bifidum, L. rhamnosus,and E. faecium with human and porcine neutrophils leads to their apoptosis.

• MeSH
• apoptóza MeSH
• Bifidobacterium bifidum fyziologie   imunologie   MeSH
• Enterococcus faecium fyziologie   imunologie   MeSH
• Lacticaseibacillus rhamnosus fyziologie   imunologie   MeSH
• lidé MeSH
• neutrofily MeSH
• probiotika * aplikace a dávkování   farmakologie   metabolismus   MeSH
• průtoková cytometrie metody   MeSH
• Sus scrofa MeSH
• techniky in vitro MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• zvířata MeSH

• Publikační typ
• abstrakt z konference MeSH

Pigs are considered as one of the major sources of zoonotic strains of Salmonella enterica for humans. Out of many S. enterica serovars, S. Typhimurium dominates in pigs, however, in several countries in Central Europe, S. Enteritidis is also quite frequent in pig herds. In this study we therefore compared the colonisation of pigs with S. Typhimurium and S. Enteritidis. We found that 3 weeks after infection S. Enteritidis 147 colonised the intestinal tract in higher quantities but was shed in faeces in lower quantities than S. Typhimurium 17C10. In a second experiment we found out that S. Enteritidis 147 and its SPI-1 and SPI-4 mutants increased proinflammatory cytokine (IL-1β and IL-8) signalling in the ileum 5 days post infection. On the other hand, independent of SPI-1 or SPI-4, S. Enteritidis 147 suppressed expression of IL-18, MCP1, TLR2, CD86, IL-7, IL-10 and IL-15 in the palatine tonsils. The suppression of cytokine signalling may facilitate the initial colonisation of the palatine tonsils by Salmonella. Moreover, immune suppression may also influence pig resistance to opportunistic pathogens and Salmonella infection in pigs thus may become an issue not only in terms of pork contamination but also in terms of affecting the immunological status of pig herds.

• MeSH
• cytokiny imunologie   metabolismus   MeSH
• krční mandle imunologie   mikrobiologie   MeSH
• lidé MeSH
• maso MeSH
• nemoci prasat imunologie   mikrobiologie   MeSH
• prasata MeSH
• Salmonella enteritidis metabolismus   fyziologie   MeSH
• Salmonella typhimurium metabolismus   fyziologie   MeSH
• salmonelová infekce u zvířat imunologie   mikrobiologie   MeSH
• Sus scrofa MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Evropa MeSH

Very little is known about the occurrence of immune system cells in the canine uterus. The aim of this study was to generate information about lymphocyte subsets that are present in the healthy canine uterus and that are recruited under inflammatory conditions caused by pyometra. Using immunohistochemistry and flow cytometry, a significant influx of γδ T lymphocytes was found in pyometra samples mainly due to recruitment of γδ(+)/CD8(-) T lymphocytes. The relative expression of genes encoding selected cytokines/chemokines was evaluated in samples from healthy and pyometra-affected uteri. Expression of pro-inflammatory cytokines (including IL-1β, TNF-α, IL-8, IL-17 and IFN-γ) and chemokines (including CXCL10, CCL4 and CCL5) was upregulated in pyometra samples confirming the presence of inflammation. In contrast, the expression of the homeostatic chemokine CCL25 and of the anti-inflammatory cytokine IL-10 was downregulated and unchanged, respectively.

• MeSH
• chemokiny genetika   imunologie   metabolismus   MeSH
• cytokiny genetika   imunologie   metabolismus   MeSH
• imunohistochemie veterinární   MeSH
• kvantitativní polymerázová řetězová reakce veterinární   MeSH
• nemoci psů imunologie   metabolismus   MeSH
• průtoková cytometrie veterinární   MeSH
• psi genetika   imunologie   metabolismus   MeSH
• pyometra imunologie   metabolismus   veterinární   MeSH
• regulace genové exprese MeSH
• T-lymfocyty - podskupiny imunologie   metabolismus   MeSH
• uterus imunologie   metabolismus   patofyziologie   MeSH
• zvířata MeSH
• Check Tag
• psi genetika   imunologie   metabolismus   MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

The protective role of hyperimmune serum in the prevention of Haemophilus parasuis infections in post-weaned piglets was assessed by experimental challenge. The hyperimmune serum was obtained from a pig vaccinated with a commercial vaccine against Glässer's disease. Thirty-eight weaned piglets were divided into four groups: three groups were immunised intramuscularly with 10 ml of hyperimmune serum and one group consisted of unimmunised control animals. All piglets were subsequently infected intraperitoneally with H. parasuis serotype 5 at different times after immunisation. The use of hyperimmune serum provided the piglets with partial protection against experimental infection. The levels of protection indirectly depend on time between serum inoculation and challenge infection. The best protection of piglets against experimental infection was obtained in the group immunised 1 week before inoculation; the same group in which the highest levels of antibodies were detected at the time of challenge.

• MeSH
• ELISA veterinární   MeSH
• Haemophilus parasuis imunologie   izolace a purifikace   MeSH
• hemofilové infekce imunologie   prevence a kontrola   veterinární   MeSH
• hemofilové vakcíny imunologie   MeSH
• lidé MeSH
• nemoci prasat imunologie   prevence a kontrola   MeSH
• odstavení MeSH
• pasivní imunizace metody   MeSH
• polymerázová řetězová reakce veterinární   MeSH
• prasata MeSH
• protilátky bakteriální krev   MeSH
• Sus scrofa MeSH
• tkáňová distribuce MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• hodnotící studie MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Česká republika MeSH