Psoriasis and metabolic syndrome (MetS), a common comorbidity of psoriasis, are associated with mild chronic systemic inflammation that increases oxidative stress and causes cell and tissue damage. At the cellular level, chromosomal and DNA damage has been documented, thus confirming their genotoxic effect. The main objective of our study was to show the genotoxic potential of chronic inflammation and determine whether the presence of both pathologies increases chromosomal damage compared to psoriasis alone and to evaluate whether there are correlations between selected parameters and chromosomal aberrations in patients with psoriasis and MetS psoriasis. Clinical examination (PASI score and MetS diagnostics according to National Cholesterol Education Expert Panel on Detection, Evaluation, and Treatment of High Blood Cholesterol in Adults; NCE/ATPIII criteria), biochemical analysis of blood samples (fasting glucose, total cholesterol, low density and high density lipoproteins; LDL, HDL, non-HDL, and triglycerides;TAG), DNA/RNA oxidative damage, and chromosomal aberration test were performed in 41 participants (20 patients with psoriasis without MetS and 21 with MetS and psoriasis). Our results showed that patients with psoriasis without metabolic syndrome (nonMetS) and psoriasis and MetS had a higher rate of chromosomal aberrations than the healthy population for which the limit of spontaneous, natural aberration was <2%. No significant differences in the aberration rate were found between the groups. However, a higher aberration rate (higher than 10%) and four numerical aberrations were documented only in the MetS group. We found no correlations between the number of chromosomal aberrations and the parameters tested except for the correlation between aberrations and HDL levels in nonMetS patients (rho 0.44; p < 0.02). Interestingly, in the MetS group, a higher number of chromosomal aberrations was documented in non-smokers compared to smokers. Data from our current study revealed an increased number of chromosomal aberrations in patients with psoriasis and MetS compared to the healthy population, especially in psoriasis with MetS, which could increase the genotoxic effect of inflammation and the risk of genomic instability, thus increasing the risk of carcinogenesis.
- Publikační typ
- časopisecké články MeSH
Polycyclic aromatic hydrocarbons (PAHs) and ultraviolet radiation (UV) represent genotoxic factors that commonly occur in the living and working environment. The dermal form of exposure represents a significant part of the total load of dangerous chemical and physical environmental factors to which an organism is subjected. However, simultaneous dermal exposures to PAHs (pharmaceutical crude coal tar [CCT]) and UV (UVA and UVB) also have therapeutic uses. A typical example is Goeckerman therapy (GT) for psoriasis. The question of the therapeutic efficacy of GT and the related level of genotoxic danger is still under discussion. The aim of the present study was to compare four GT variants (G1-G4) in terms of efficacy and acceptable genotoxic hazard. Efficacy was expressed by the psoriasis area of severity index (PASI) score, genotoxic hazard by chromosomal aberration in peripheral lymphocytes. The lowest risk of genotoxic hazard and the lowest efficiency was observed in G1 variant (3% of the CCT and UVA + UVB). The efficacy of G2 (4% CCT and UVA + UVB), G3 (4% CCT and UVB), and G4 variants (5% CCT and UVA + UVB) was comparable. The highest risk of genotoxic hazard was found in the G3 variant. In the terms of sufficient efficacy and acceptable genotoxic hazard, a combination of 4% or 5% of CCT and UVA and UVB seems to be acceptable (variants G2 and G4).
- MeSH
- chromozomální aberace MeSH
- dehet uhelný terapeutické užití MeSH
- lidé MeSH
- lymfocyty MeSH
- polycyklické aromatické uhlovodíky toxicita MeSH
- poškození DNA MeSH
- psoriáza farmakoterapie MeSH
- ultrafialové záření * MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
OBJECTIVE: The two main aims of our study were to assess the quality of life (QOL) of patients with moderate or severe psoriasis treated by Goeckerman therapy and to compare QOL of our patients on the date of admission and 1 month after their discharge. METHODS: We performed a prospective study on 51 patients treated for chronic plaque psoriasis by Goeckerman regimen (GR). The psoriasis area involvement and severity was measured using the Psoriasis Area and Severity Index (PASI). Patients' health-related QOL was evaluated using Psoriasis Disability Index (PDI). RESULTS: In our study we demonstrated that the QOL of this sample of 51 patients with severe forms of psoriasis was relatively good; an average PDI value on the day of admission was 9.02, which represents a small effect on patient's QOL. We did not find any significant differences in QOL between younger and older respondents or between men and women. Despite good treatment efficiency, we did not find improvement in QOL 1 month after discharge. CONCLUSIONS: The QOL of our patients was relatively good. This could be explained by the fact that our patients have been suffering from psoriasis for many years and have probably adapted to their disease. QOL of our patients was not directly dependent on the extent and intensity of skin changes. Further studies on the connection between psoriasis therapy and patients' QOL are still highly needed.
- MeSH
- kvalita života * MeSH
- lidé MeSH
- prospektivní studie MeSH
- psoriáza terapie MeSH
- stupeň závažnosti nemoci MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
OBJECTIVE: Early morning shifts have a negative effect on the hypothalamic-pituitary-adrenal axis. The aim of this study was to comprehensively assess the extent of occupational stress in early shift workers of the car industry by using salivary cortisol as an objective marker during a workday and on a day off. METHODS: For this cross-sectional epidemiological type of study, a survey included 55 suitable volunteers from the car industry. Five saliva samples were collected according to the following schedule: during one work day in the morning, during the morning shift from 6 a.m. - 2 p.m., then after the shift was completed, 3 hours after work and in the evening before going to sleep. Control samples were taken from the same participants on a day off. Radioimmunoanalysis was used as the main analytical method, and the effect of factors and between-factor interactions on the levels of salivary cortisol during the workday were assessed using an ANOVA model. RESULTS: The cortisol diurnal rhythm was as expected, with the highest values in the morning and declining to the lowest values in the evening hours. Concentrations of salivary cortisol showed higher values during the workday, especially higher concentrations of evening cortisol and attenuated cortisol slope. Based on the results, irregular shift work has a greater increase in cortisol excretion after waking in the morning and a slower progressive recovery of the organism during the workday. In addition, cortisol levels were significantly higher in older women than in older men but did not differ in younger subjects. CONCLUSIONS: Salivary cortisol levels are a suitable objective marker of stress and can be used as a good predictor of occupational stress by public health services for the purposes of primary prevention.
- MeSH
- cirkadiánní rytmus fyziologie MeSH
- dospělí MeSH
- hydrokortison metabolismus MeSH
- lidé MeSH
- pracovní stres metabolismus MeSH
- průřezové studie MeSH
- sliny chemie MeSH
- vliv směnného provozu na zdraví fyziologie MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika MeSH
OBJECTIVE: The aim of this study is to monitor and analyze the influence of chosen family indicators related to alcohol consumption and experience of drunkenness in a representative sample of Czech children. METHODS: Data for our work were obtained from the Health Behaviour in School Aged Children (HBSC) study - a World Health Organization (WHO) cross national study. The sample consisted of 4,293 children aged 11, 13 and 15 years. Data collection was conducted in June 2010 in 88 randomly selected schools in the Czech Republic using standardized questionnaires. Statistical analysis was performed in the program NCSS 9, methods of descriptive statistics, χ(2) test of independence in the contingency tables and logistic regression analysis were used. RESULTS: Regular alcohol consumption (at least weekly) was reported by 7.4% of children aged eleven, 19.3% of children aged thirteen and 38.4% of children aged fifteen years. Drunkenness at least twice in their life was admitted by 3% of children aged eleven, 15% of children aged thirteen and 43% of children aged fifteen years. Both alcohol consumption and drunkenness were significantly associated (p<0.001) with age and gender. Formal structure of family was found to have significant effect on the experience of drunkenness, but no effect on alcohol consumption was recorded. Based on our results, neither the amount of time spent together, nor the children communication with parents had a statistically significant influence on alcohol consumption or drunkenness experience within each family type. However, statistically significant differences were observed between different family types (p<0.001). CONCLUSIONS: These findings indicate a high degree of liberalism of Czech society towards alcohol. It appears that alcohol consumption will remain a serious problem in Czech society, therefore, more attention should be paid to this phenomenon in the future.
- MeSH
- charakteristiky rodiny * MeSH
- dítě MeSH
- lidé MeSH
- mladiství MeSH
- pití alkoholu epidemiologie MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- mladiství MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika epidemiologie MeSH
The purpose of the study was to describe the occurrence of the most common complaints related to MSDs in Czech dentists and to assess the risk factors affecting them. A questionnaire survey of 581 Czech dentists (the response rate 72.6%) was conducted in 2011. The questionnaire ascertained general information about the respondents, their work habits and environment along with the occurrence of musculoskeletal disorders. The respondents filled out the questionnaires during the educational events organized by the Czech Dental Chamber. At least mild difficulties associated with the motoric system were reported by 96.9% of the respondents, with 66.3% of respondents reporting moderate or major difficulties. Back and neck pain followed by shoulder pain and headache were the most common complaints in our sample. According to our data: age, gender, length of practice, a history of serious MSDs, the occurrence of MSDs in blood relatives, the perception of work as psychologically demanding, and especially a perceived moderate/bad general health were significantly associated with the four most common musculoskeletal complaints. Some of the factors were found as protective. This study suggests that MSDs represent a significant burden for Czech dentists and further research is needed to elucidate this issue.
- MeSH
- bolesti hlavy epidemiologie MeSH
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladý dospělý MeSH
- muskuloskeletální bolest epidemiologie MeSH
- muskuloskeletální nemoci epidemiologie genetika MeSH
- ochranné faktory MeSH
- pracovní zátěž psychologie MeSH
- průřezové studie MeSH
- rizikové faktory MeSH
- senioři MeSH
- sexuální faktory MeSH
- věkové faktory MeSH
- zdravotní stav MeSH
- zpráva o sobě MeSH
- zubní lékaři statistika a číselné údaje MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika epidemiologie MeSH
x
x
- MeSH
- charakteristiky rodiny MeSH
- dítě MeSH
- kvalita života * MeSH
- lidé MeSH
- mladiství MeSH
- osobní uspokojení MeSH
- průřezové studie MeSH
- průzkumy a dotazníky MeSH
- regresní analýza MeSH
- rodinné vztahy * MeSH
- vztahy mezi rodiči a dětmi * MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- mladiství MeSH
- Publikační typ
- práce podpořená grantem MeSH
Cíl: Cílem práce bylo sledovat prevalenci kouření a následně analyzovat souvislost mezi kouřením a vybranými aspekty rodiny u reprezentativního vzorku českých dětí. Metodika: Sledovaný soubor činil 4328 dětí ve věku 11, 13 a 15 let z 88 náhodně vybraných škol České republiky. Data byla získána v rámci mezinárodní studie „The Health Behaviour in School-aged Children: A WHO Cross National Study (HBSC) v červnu roku 2010 prostřednictvím standardizovaného dotazníku. Statistická analýza dat byla provedena v programu NCSS 9. Výsledky: Pravidelné kouření (alespoň jednou týdně) uvedlo 1,6 % dětí jedenáctiletých, 9,7 % třináctiletých a 24,4 % dětí patnáctiletých. Rozdíly mezi věkovými kategoriemi byly statisticky významné na p < 0,001. Z hlediska pohlaví byl statisticky významný rozdíl (p < 0,05) prokázán pouze u skupiny patnáctiletých dětí, a to v neprospěch dívek. Formální struktura rodiny statisticky významně (p < 0,001) souvisela s prevalencí kuřáctví. Nejméně často pravidelné kouření uvedly děti z úplné rodiny, nejčastěji děti žijící bez rodičů. Statisticky významný vztah mezi kouřením a množstvím společně tráveného času dětí s rodinou ani kvalitou komunikace dětí s rodiči v naší práci nebyl prokázán. Závěr: Prevalence kouření českých dětí je vysoká. K omezení počtu kuřáků je třeba se zaměřit na primární prevenci.
Objectives: The aim of our paper was to monitor the prevalence of smoking and to analyse the relation among chosen family indicators and smoking in a representative sample of Czech children. Methods: A total number of 4.328 children aged 11, 13 and 15 years out of 88 randomly selected schools in the Czech Republic formed the study population. The data were obtained within the WHO study „The Health Behaviour in School-aged Children: A WHO Cross National Study“ (HBSC) in June 2010 by means of standardized questionnaires. Statistical analysis was performed using the NCSS 2007 program. Results: Regular smoking (at least once a week) was reported by 1.6% of children aged 11, 9.7% of children aged 13 and 24.4% of children aged fifteen. Differences between age groups were statistically significant at p < 0.001. In terms of gender, significant association (p < 0.05) was found only in children aged 15, to the detriment of girls. Formal structure of family was found to be significantly associated (p < 0.001) with smoking. Regular smoking was reported least frequently by children from complete families and most frequently by children living without parents. Neither the amount of time spent together with a family nor the quality of communication with parents were found to have significant effect on smoking. Conclusions: The prevalence of regular smokers among Czech children is high. It is important to direct the attention to the primary prevention to decrease the amount of smokers.
- MeSH
- charakteristiky rodiny MeSH
- dítě MeSH
- kouření * epidemiologie MeSH
- lidé MeSH
- mladiství MeSH
- postoj MeSH
- prevalence MeSH
- průzkumy a dotazníky MeSH
- rodina * MeSH
- statistika jako téma MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- mladiství MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
BACKGROUND: It's indicated negative-perceived stress could induce worse health status and change of cortisol secretion. OBJECTIVES: To assess salivary cortisol levels in two occupations with a high psychosocial workload, but different features, teachers and firefighters. METHODS: The study population consisted of 142 school teachers and 136 firefighters. Four saliva samples were collected from pedagogical participants during their busiest workday. The cortisol measures used were: morning values, evening values, slope of decline, ratio (evening value divided by morning value), and area under the curve (AUC). RESULTS: The salivary cortisol measurements in both genders were almost equal regarding morning values, slope, and AUC increase. Evening values were lower and the relative reactivity was higher (lower ratio) for female teachers, compared to male teachers. There was a tendency of a lower total daytime output of cortisol (AUC ground) among female teachers. Firefighters had lower levels of cortisol, lower total daytime output, and higher relative reactivity (lower ratio), but lower absolute reactivity, regarding both slope and AUC increase. CONCLUSION: Overall, male teachers might be the group most affected by stress in this study, even if some of their cortisol values were almost equal to the female teachers' values. Male teachers also seemed to be more affected by stress, according to salivary cortisol, compared to male firefighters, even if there were some inconsistencies.
- MeSH
- akademický sbor * MeSH
- dospělí MeSH
- hasiči * MeSH
- hydrokortison biosyntéza MeSH
- lidé středního věku MeSH
- lidé MeSH
- nemoci z povolání etiologie metabolismus MeSH
- psychický stres metabolismus MeSH
- sexuální faktory MeSH
- sliny chemie MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- MeSH
- akademie a ústavy * dějiny klasifikace organizace a řízení využití MeSH
- bezpečnost potravin MeSH
- environmentální zdraví MeSH
- epidemiologické studie MeSH
- genetika MeSH
- hygiena * MeSH
- kvalita vody normy MeSH
- kvalita života MeSH
- lidé MeSH
- mezinárodní spolupráce MeSH
- preventivní lékařství * dějiny klasifikace metody organizace a řízení pracovní síly trendy MeSH
- rizikové faktory MeSH
- rozvoj plánování MeSH
- toxikologie MeSH
- výzkum MeSH
- vzdělávání odborné MeSH
- zdravotní stav MeSH
- životní styl MeSH
- znečištění ovzduší MeSH
- Check Tag
- lidé MeSH
