The cytotoxic properties of zinc nanoparticles have been evaluated in vitro against several types of cancer. However, there is a lack of significant evidence of their activity in vivo, and a potential therapeutic application remains limited. Herein we report the effective inhibition of tumor growth by zinc nanoparticles in vivo, as the effect of the dietary intervention, after the chemical induction in a rodent model of breast cancer. Biopsy images indicated grade 1 tumors with multiple inflammatory infiltrates in the group treated with zinc nanoparticles, whereas, in the other groups, a moderately differentiated grade 2 adenocarcinoma was identified. Moreover, after the supplementation with zinc nanoparticles, the levels of several metabolites associated with cancer metabolism, important to its survival, were found to have been altered. We also revealed that the biological activity of zinc in vivo depends on the size of applied particles, as the treatment with zinc microparticles has not had much effect on cancer progression.
- MeSH
- adenokarcinom metabolismus patologie prevence a kontrola MeSH
- kovové nanočástice aplikace a dávkování analýza MeSH
- krysa rodu rattus MeSH
- nádory prsu metabolismus patologie prevence a kontrola MeSH
- nanotechnologie MeSH
- oxid zinečnatý chemie MeSH
- potkani Sprague-Dawley MeSH
- potravní doplňky MeSH
- protinádorové látky aplikace a dávkování analýza MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
We studied the surface properties and cytocompatibil-ity of a grafted biopolymer, poly(3-hydroxybutyrate) (P3HB). P3HB was exposed to an inert argon plasma dis-charge, then grafted by 1,1 '-biphenyl-4,4 '-dithiol (BFD) and finally grafted by golden nanoparticles (nanospheres and nanorods). The surface properties were studied using multiple methods – goniometry, atomic force microscopy and X-ray photoelectron spectroscopy. Cytocompatibility was determined in vitro by studying adhesion, prolifera-tion and viability of vascular smooth muscle cells (VSMCs) from the aorta of Rattus norvegicus. The cyto-compatibility was compared for pristine, modified P3HB and standard tissue culture polystyrene (TCPS). Our re-sults show that surface morphology and wettability are affected by both plasma discharge and nanoparticles graft-ing. These changes suggest that the adhesion and prolifera-tion of VSMCs is enhanced more on the plasma modified and grafted substrate.
Surface-modified gold multibranched nanoparticles (AuMs) were prepared by simple chemical reduction of gold chloride aqueous solution followed by in situ modification by using water-soluble arenediazonium tosylates with different functional organic groups. Chemical and morphological structures of the prepared nanoparticles were examined by using transmission electron and scanning electron microscopies. The covalent grafting of organic compounds was confirmed by scanning electron microscopy with energy dispersive X-ray spectroscopy (SEM-EDX) and Raman spectroscopy techniques. Covalent functionalization of nanoparticles significantly expands the range of their potential uses under physiological conditions, compared with traditional non-covalent or thiol-based approaches. The antibacterial effect of the surface-modified AuMs was evaluated by using Escherichia coli and Staphylococcus epidermidis bacteria under IR light illumination and without external triggering. Strong plasmon resonance on the AuMs cups leads to significant reduction of the light power needed kill bacteria under the mild conditions of continuous illumination. The effect of the surface-modified AuMs on the light-induced antibacterial activities was founded to be dependent on the grafted organic functional groups.
Nanomaterials are in analytical science used for a broad range of purposes, covering the area of sample pretreatment as well as separation, detection, and identification of target molecules. This part of the review covers capillary electrophoresis (CE) of nanomaterials and focuses on the application of CE as a method for characterization used during nanomaterial synthesis and modification as well as the monitoring of their properties and interactions with other molecules. The heterogeneity of the nanomaterial family is extremely large. Depending on different definitions of the term Nanomaterial/Nanoparticle, the group may cover metal and polymeric nanoparticles, carbon nanomaterials, liposomes and even dendrimers. Moreover, these nanomaterials are usually subjected to some kind of surface modification or functionalization, which broadens the diversity even more. Not only for purposes of verification of nanomaterial synthesis and batch-to-batch quality check, but also for determination the polydispersity and for functionality characterization on the nanoparticle surface, has CE offered very beneficial capabilities. Finally, the monitoring of interactions between nanomaterials and other (bio)molecules is easily performed by some kind of capillary electromigration technique.
Príspevok sa venuje analýze rôznych frakcií aerosólu vrátane nanometrických, mikrometrických častíc a PM "particulate matter" častíc v pracovnom ovzduší. Z výsledkov vyplýva, že analýzou veľkostnej distribúcie častíc pri výrobe oceľových rúr valcovaním za tepla, konkrétne pri dierovacom lise, sa preukázala prítomnosť PM častíc s rozmermi menšími ako 0,5 μm (7,1 μg/m3), ako 1 μm (16,4 μg/m3), ako 2,5 μm (40,4 μg/m3), ako 4,0 μm (58,6 μg/m3) a ako 10 μm (95,1 μg/m3). Ďalej sme výsledky vyhodnocovali ako veľkostnú distribúciu častíc podľa ich početnosti. Z celkového počtu častíc v odobratom objeme vzduchu 4,90⋅105 bolo 4,13⋅105 nanočastíc (84,4 %). Na druhom pracovisku – pri tŕňovej tyči – sme analyzovali podobne PM častice. Výsledky preukázali prítomnosť PM0,5 (2,8 μg/m3), PM1,0 (3,9 μg/m3), PM2,5 (15,5 μg/m3) a PM10 (119,9 μg/m3). V odobratom objeme vzduchu bolo z celkového počtu 5,75⋅105 častíc 5,29⋅105 nanočastíc (92,1 %). Z nameraných hodnôt vyplýva, že je dôležité objektivizovať častice v pracovnom ovzduší aj v nanorozmeroch a mikrorozmeroch, pretože môžu predstavovať potenciálne riziko poškodenia zdravia.
The article deals with the analysis of various aerosol fractions, including nanometric, micrometric particles and particulate matter (PM), in the working atmosphere. Analysis of particle distribution in the production of steel tubes by hot rolling, precisely at the punching press has been shown the presence of PM particles with diameter less than 0.5 μm (7.1 μg/m3), 1 μm (16.4 μg/m3), 2.5 μm (40.4 μg/m3), less than 4.0 μm (58.6 μg/m3) and less than 10 μm (95.1 μg/m3). We further evaluated the results of particle size distribution according to their quantity. We have analysed of the total number of particles in the withdrawn air volume of 4.90⋅105. The results showed that 84.4% (4.13⋅105) of the total number of particles is in the range of nanometres. In the another workplace with the thorn rods production, the results showed the presence of PM0.5 (2.8 μg/m3), PM1.0 (3.9 μg/m3), PM2.5 (15.5 μg/m3) and PM10 (119.9 μg/m3). The total number of particles in the nanometre range was 5.29⋅105 (92.1%). The total number of particles of this analysis of the air was 5.75⋅105. From the measured values, it is important to objectify the particles in the working atmosphere even in nano and micro dimensions, as they may pose a potential risk of damage to health.
Silver nanoparticles (AgNP) are emerging pollutants. The use of novel materials such as Cu-(benzene 1,3,5-tricarboxylate, BTC) Metal-Organic Framework (MOFs), for AgNP adsorption and their removal from aqueous solutions has been studied. The effect of different parameters was followed and isotherm model was suggested. MOFs adsorbed fast and efficiently AgNP in the range C0 < 10 mg L(-1), being Freundlich isotherm (R = 0.993) these data fitted to. Among studied parameters a remarkable effect of chloride on sorption was found, thus their possible interactions were considered. The high adsorption efficiency of AgNP was achieved and it was found to be very fast. The feasibility of adsorption on Cu-(BTC) was proved in spiked waters. The results showed the potential interest of new material as adsorbent for removing AgNP from environment.
This study aimed to evaluate the palatine tonsils of patients with chronic tonsillitis and spinocellular carcinoma to determine the presence of nano-sized particles. Tonsil samples from adult patients with chronic tonsillitis and spinocellular carcinoma of the palatine tonsil were dried and analyzed using a scanning electron microscope with the X-ray microprobe of an energy-dispersive spectroscope. Demographic data and smoking histories were obtained. The principal metals found in almost all tissues analyzed were iron, chromium, nickel, aluminum, zinc, and copper. No significant difference in elemental composition was found between the group of patients with chronic tonsillitis and the group with spinocellular carcinoma of the palatine tonsil. Likewise, no significant difference was found between the group of smokers and the group of nonsmokers. The presence of various micro- and nano-sized metallic particles in human tonsils was confirmed. These particles may potentially cause an inflammatory response as well as neoplastic changes in human palatine tonsils similar to those occurring in the lungs. Further and more detailed studies addressing this issue, including studies designed to determine the chemical form of the metals detected, studies devoted to quantitative analysis, biokinetics, and to the degradation and elimination of nanoparticles are needed for a more detailed prediction of the relation between the diagnosis and the presence of specific metal nanoparticles in tonsillar tissue.
- MeSH
- chronická nemoc MeSH
- dospělí MeSH
- kovové nanočástice analýza MeSH
- krční mandle chemie MeSH
- lidé středního věku MeSH
- lidé MeSH
- mikroskopie elektronová rastrovací MeSH
- mladý dospělý MeSH
- senioři MeSH
- spinocelulární karcinom chemie MeSH
- tonzilární nádory chemie MeSH
- tonzilitida metabolismus MeSH
- vystavení vlivu životního prostředí MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladý dospělý MeSH
- senioři MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
