The porcine aorta is often used in studies on morphology, pathology, transplantation surgery, vascular and endovascular surgery, and biomechanics of the large arteries. Using quantitative histology and stereology, we estimated the area fraction of elastin, collagen, alpha-smooth muscle actin, vimentin, and desmin within the tunica media in 123 tissue samples collected from five segments (thoracic ascending aorta; aortic arch; thoracic descending aorta; suprarenal abdominal aorta; and infrarenal abdominal aorta) of porcine aortae from growing domestic pigs (n=25), ranging in age from 0 to 230 days. The descending thoracic aorta had the greatest elastin fraction, which decreased proximally toward the aortic arch as well as distally toward the abdominal aorta. Abdominal aortic segments had the highest fraction of actin, desmin, and vimentin positivity and all of these vascular smooth muscle markers were lower in the thoracic aortic segments. No quantitative differences were found when comparing the suprarenal abdominal segments with the infrarenal abdominal segments. The area fraction of actin within the media was comparable in all age groups and it was proportional to the postnatal growth. Thicker aortic segments had more elastin and collagen with fewer contractile cells. The collagen fraction decreased from ascending aorta and aortic arch toward the descending aorta. By revealing the variability of the quantitative composition of the porcine aorta, the results are suitable for planning experiments with the porcine aorta as a model, i.e. power test analyses and estimating the number of samples necessary to achieving a desirable level of precision. The complete primary morphometric data, in the form of continuous variables, are made publicly available for biomechanical modeling of site-dependent distensibility and compliance of the porcine aorta.
- MeSH
- aktiny metabolismus MeSH
- aorta abdominalis růst a vývoj ultrastruktura MeSH
- aorta thoracica růst a vývoj ultrastruktura MeSH
- aorta růst a vývoj ultrastruktura MeSH
- desmin metabolismus MeSH
- elastin metabolismus MeSH
- imunohistochemie MeSH
- kolagen metabolismus MeSH
- novorozená zvířata MeSH
- prasata MeSH
- stárnutí fyziologie MeSH
- Sus scrofa MeSH
- svalová kontrakce fyziologie MeSH
- svaly hladké cévní růst a vývoj ultrastruktura MeSH
- tunica media růst a vývoj ultrastruktura MeSH
- vimentin metabolismus MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Alterations in geometry and structure of coronary arteries have marked consequences on blood flow to the respective area. We evaluated long-term effect of losartan on blood pressure (BP), heart weight/body weight (HW/BW), geometry and structure of septal branch of coronary artery (RS) of young SHR and Wistar rats. Four-week-old Wistar rats and SHR were used. Losartan was administered (20 mg/kg/day) in drinking water by gavage for 5 weeks. BP was measured by plethysmographic method. Cardiovascular system was perfused with a fixative (120 mm Hg). RS was processed for electron microscopy. Wall thickness of intima + media (WT), inner diameter (ID), cross-sectional area of intima + media (CSA), volume densities (VD) of endothelial cells (EC), extracellular matrix (ECM) of intima, smooth muscle cells (SMC) and ECM of media were evaluated. BP of 4-week-old SHR did not differ from that of Wistar rats. BP, HW/BW, WT, CSA, WT/ID, CSAs of SMC, ECM of media were increased in 9-week-old SHR, whereas their VD and CSA of EC were decreased. Losartan administration decreased BP and HW/BW in both groups. Geometry of RS was affected only in SHR (reduction of WT, CSA, WT/ID and increased of ID, circumferential tension, VD and CSA of EC). Losartan administration reduced BP and myocardial mass in both groups and beneficially affected geometry and structure of coronary artery in SHR.
- MeSH
- antihypertenziva aplikace a dávkování MeSH
- blokátory receptoru 1 pro angiotenzin II aplikace a dávkování MeSH
- financování organizované MeSH
- hypertenze MeSH
- kardiomegalie MeSH
- koronární cévy účinky léků ultrastruktura MeSH
- krevní tlak účinky léků MeSH
- krysa rodu rattus MeSH
- losartan antagonisté a inhibitory aplikace a dávkování MeSH
- modely nemocí na zvířatech MeSH
- myokard patologie MeSH
- potkani inbrední SHR MeSH
- potkani Wistar MeSH
- rozvrh dávkování léků MeSH
- srdce účinky léků MeSH
- tunica intima účinky léků ultrastruktura MeSH
- tunica media účinky léků ultrastruktura MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- zvířata MeSH
BACKGROUND: Serum bilirubin is negatively associated with the development of coronary heart disease. In the present study, we have focused on the analysis of intima-media thickness (IMT) of the common carotid artery in hyper- and normobilirubinemic subjects. METHODS: The study was performed on 111 men without manifested atherosclerosis. In all subjects, complete biochemical tests were determined along with the examination of IMT by carotid ultrasound. RESULTS: The mean IMT in hyperbilirubinemic subjects as compared with controls was substantially lower (p=0.017), and hyperbilirubinemic men also had very low age-adjusted prevalence odds ratios for having IMT above the 50th percentiles of controls, even after adjustment for selected vascular risk factors (p=0.034). CONCLUSIONS: In the present study, we demonstrate the inverse relationship between serum bilirubin and IMT in healthy men. Copyright (c) 2006 S. Karger AG, Basel.
- MeSH
- arteria carotis communis patologie ultrasonografie MeSH
- bilirubin krev MeSH
- biologické markery krev MeSH
- dospělí MeSH
- financování organizované MeSH
- Gilbertova nemoc krev patologie MeSH
- jaterní testy MeSH
- lidé středního věku MeSH
- lidé MeSH
- senioři MeSH
- studie případů a kontrol MeSH
- tunica intima patologie ultrasonografie MeSH
- tunica media patologie ultrastruktura MeSH
- věkové faktory MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
Cytosol levels of cytokines [interleukins 1b, 6, 8 (IL-1b, 6, 8), tumor necrosis factor-alpha (TNF-alpha)] in aneurysm walls were evaluated in a prospective non-randomized study of 57 patients. The group was divided into two subgroups: Subgroup I (ruptured aneurysms, n=11) and Subgroup II (asymptomatic aneurysms, n=32). A control group consisted of 14 kidney donors. Aortic walls were examined by immunohistochemistry and microscopy to detect inflammatory cells. More pronounced inflammatory changes and higher cytosol cytokine levels [IL6 (p<0.001), IL8 (p<0.0003) and TNFalpha (p<0.002)] were found in the walls of ruptured aneurysms than in the asymptomatic aneurysms. Immunohistochemically, most cells within the inflammatory infiltrates stained positively with the monoclonal antibody to the leucocyte common antigen (CD 45). The majority were of B-cell origin, which was demonstrated by positive staining with the monoclonal antibody L26 directed against the CD 20 antigen. These results show that an inflammatory process plays a significant role in patients with ruptured abdominal aortic aneurysms (AAA). A means of modifying the inflammatory process in the wall of AAAs might play an important role in preventing aneurysm rupture.
- MeSH
- aneurysma břišní aorty imunologie komplikace metabolismus patologie MeSH
- antigeny CD20 metabolismus MeSH
- antigeny CD45 metabolismus MeSH
- cytokiny analýza MeSH
- interleukin-6 analýza MeSH
- interleukin-8 analýza MeSH
- lidé středního věku MeSH
- lidé MeSH
- monoklonální protilátky metabolismus MeSH
- plazmatické buňky ultrastruktura MeSH
- prasklé aneurysma chirurgie MeSH
- prospektivní studie MeSH
- senioři MeSH
- TNF-alfa analýza MeSH
- tunica media patologie ultrastruktura MeSH
- zánět * imunologie MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- srovnávací studie MeSH
