Huang, Xu* Dotaz Zobrazit nápovědu
Vydání první 165 stran : ilustrace, tabulky ; 27 cm
Vhodným poklepáváním určitých bodů či částí těla a správnou sérií cviků lze rozproudit energii těla i ducha a dosáhnout posílení celého organismu. Kniha je návodem, jak akupresuru poklepem správně provádět. Cviky v ní obsažené jsou vhodné jako doplněk k léčbě zdravotních obtíží, ale i pro zachování zdraví a prevenci nemocí.
- Klíčová slova
- Acupoint Tapping,
- MeSH
- akupresura metody MeSH
- cvičení MeSH
- techniky cvičení a pohybu metody MeSH
- tradiční čínská medicína metody MeSH
- Publikační typ
- populární práce MeSH
- příručky MeSH
382 s. ; 25 cm
- MeSH
- akupunkturní terapie dějiny klasifikace metody využití MeSH
- dějiny starověku MeSH
- komplementární terapie dějiny klasifikace metody využití MeSH
- tradiční čínská medicína dějiny metody využití MeSH
- Check Tag
- dějiny starověku MeSH
- Geografické názvy
- Čína MeSH
- Konspekt
- Fyzioterapie. Psychoterapie. Alternativní lékařství
- NLK Obory
- alternativní lékařství
- NLK Publikační typ
- texty
Cíl: Cílem této studie bylo sledování kognitivních funkcí u pacientů při celomozkovém ozařování (whole brain radiotherapy; WBRT) pro mozkové metastázy (brain metastases; BM) karcinomu plic a analýza strategií řízení radioterapie pomocí inteligentního softwaru. Metody: Byla provedena retrospektivní analýza klinických dat 146 pacientů s BM karcinomu plic, přičemž pacienti byli rozděleni do skupiny A (n = 95, pacienti s neurologickými symptomy) a skupiny B (n = 51, pacienti bez neurologických symptomů). Byla provedena multivariační logistická regresní analýza s cílem prozkoumat rizikové faktory pro zhoršení kognitivních funkcí a byl zkonstruován a zhodnocen predikční model. Výsledky: Po WBRT se ve skupině A výrazně zvýšilo skóre tzv. krátkého testu kognitivních funkcí (Mini-Mental State Examination; MMSE) (p < 0,05), které bylo nejvyšší ve 4. měsíci a pak došlo k mírnému poklesu. Naproti tomu ve skupině B došlo po WBRT ke snížení skóre, přičemž nejnižší bylo ve 4. měsíci a pak došlo k jeho zvýšení. Logistická analýza ukázala, že nezávislými rizikovými faktory pro zhoršení kognitivních funkcí byla chemoterapie podávaná ve ≥ 3 cyklech se simultánním integrovaným boostem, ozáření v dávce > 30 Gy a absence hipokampus šetřící techniky ozařování (p < 0,05). Závěr: Technika WBRT má na BM karcinomu plic zřetelné terapeutické účinky, ale ovlivňuje kognitivní funkce, což je nejvíc patrné ve 4. měsíci léčby.
Aim: The aim of this study was to investigate cognitive functions of patients receiving whole brain radiotherapy (WBRT) for brain metastases (BM) from lung cancer, and to analyze guidance strategies based on intelligent software. Methods: The clinical data of 146 patients with BM from lung cancer were collected for retrospective analysis, and they were assigned to group A (N = 95, presence of neurological symptoms) and group B (N = 51, absence of neurological symptoms). Multivariate logistic regression analysis was employed to explore the risk factors for cognitive decline, and a prediction model was constructed and evaluated. Results: After WBRT, the Mini-Mental State Examination (MMSE) score of group A increased significantly (P < 0.05), the highest was in the 4th month and then decreased slightly. In contrast, the MMSE score of group B was lowered after WBRT, the lowest was in the 4th month and rose thereafter. Logistic analysis indicated that chemotherapy ≥ 3 cycles, simultaneous integrated boost, irradiation dose > 30 Gy and absence of hippocampal avoidance were independent risk factors for cognitive decline (P < 0.05). Conclusion: WBRT has obvious therapeutic effects on BM from lung cancer, but it influences the cognitive functions, which is the most noticeable in the 4th month.
- MeSH
- klinická studie jako téma MeSH
- kognice účinky léků účinky záření MeSH
- kognitivní porucha po chemoterapii MeSH
- kognitivní poruchy * etiologie MeSH
- lidé MeSH
- metastázy nádorů MeSH
- nádory mozku * radioterapie sekundární MeSH
- nádory plic MeSH
- radioterapie * škodlivé účinky MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- práce podpořená grantem MeSH
This study was aimed to determine whether the leptin receptor Gln223Arg polymorphism has an effect on plasma leptin levels and left ventricular hypertrophy in hypertension. The genotypes and allelic frequencies of the leptin receptor Gln223Arg were examined using the polymerase chain reaction and restriction fragment length polymorphism in 190 hypertensive patients and 88 healthy controls. Logarithmic (log) transformation of the plasma leptin level was carried out before performing comparison and regression analysis. The values of log(plasma leptin levels) in the hypertensive patients were higher than those of controls and they were higher in hypertensive patients with left ventricular hypertrophy than those without it (P < 0.05). The genotype (AA, AG, and GG) distribution of Gln223Arg polymorphism was 6.8, 33.8, and 59.4% in hypertensive patients with left ventricular hypertrophy, 4.3, 27.6, and 68.1% in patients without left ventricular hypertrophy, and 2.3, 26.1, and 71.6% in the controls, respectively. A significant difference was found among these three groups (P < 0.05). The frequency of allele A was higher in patients with left ventricular hypertrophy than in patients without it (23.6 vs. 18.1%; P < 0.05). Log(plasma leptin levels) and left ventricular mass index were higher in patients with the AA genotype than those with the AG or GG genotype in hypertensive patients (P < 0.05). In multivariate regression analysis, the AA genotype as an independent predictor had statistically significant effects on the left ventricular mass index. Our results suggest that the Gln223Arg polymorphism of the leptin receptor is significantly associated with plasma leptin levels and left ventricular hypertrophy in hypertension.
- MeSH
- dospělí MeSH
- echokardiografie MeSH
- genotyp MeSH
- hypertrofie levé komory srdeční genetika MeSH
- leptinové receptory genetika MeSH
- lidé středního věku MeSH
- lidé MeSH
- polymorfismus genetický genetika MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
To provide the bilateral advantages of emulsions and hydrogels, a facile approach was used to fabricate nanoemulsions filled hydrogel beads through combining the method of self-emulsification and sodium alginate (SA) ionic gelation. The encapsulation and release behavior of curcumin (Cur) were further investigated. The results indicated that Cur packaged nanoemulsions were with the size of 24.26 ± 0.22 nm. The nanoemulsions filled SA hydrogel beads were spherical shell with the diameter of 0.46 ± 0.02 mm. For Cur, the EE and LC of emulsion filled SA hydrogel beads were 99.15 ± 0.85% and 7.25 ± 3.16 mg/g respectively. The release behavior could be regulated by external pH condition. The release behavior at pH 9.0 displayed a higher release rate than that at pH 7.0. Cur released behavior well followed the Hixcon-Crowell model which indicated that Cur was released in a diffusion-controlled model. Comparatively investigation of microstructure using field emission scanning electron microscope (FE-SEM) further investigates the corrosion behavior of SA gel beads during Cur release. The worth-while endeavor provided a practical combined technique of emulsions and ionic gelation to fabricate hybrid hydrogel beads that have potential in delivery system for hydrophobic composition.
Nicotine has well-documented effects on the growth and colonization of Streptococcus mutans. This study attempts to investigate the effects of nicotine on pathogenic factors of S. mutans, such as the effect on biofilm formation and viability, expression of pathogenic genes, and metabolites of S. mutans. The results demonstrated that addition of nicotine did not significantly influence the viability of S. mutans cells. The biofilms became increasingly compact as the concentrations of nicotine increased. The expression of virulence genes, such as ldh and phosphotransferase system (PTS)-associated genes, was upregulated, and nlmC was upregulated significantly, while ftf was downregulated. The lactate concentration of S. mutans grown in 1 mg/mL of nicotine was increased up to twofold over either biofilm or planktonic cells grown without nicotine. Changes in the metabolites involved in central carbon metabolism from sucrose indicated that most selected metabolites were detectable and influenced by increased concentrations of nicotine. This study demonstrated that nicotine can influence the pathogenicity of S. mutans and may lead to increased dental caries through the production of more lactate and the upregulation of virulence genes.
- MeSH
- biofilmy účinky léků růst a vývoj MeSH
- exprese genu účinky léků MeSH
- faktory virulence biosyntéza MeSH
- kyselina mléčná metabolismus MeSH
- lidé MeSH
- metabolické sítě a dráhy účinky léků MeSH
- mikrobiální viabilita účinky léků MeSH
- nikotin metabolismus MeSH
- sliny mikrobiologie MeSH
- Streptococcus mutans účinky léků patogenita MeSH
- virulence účinky léků MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
The contraction of gastrointestinal (GI) smooth muscles is regulated by both Ca(2+)-dependent and Ca(2+) sensitization mechanisms. Proline-rich tyrosine kinase 2 (Pyk2) is involved in the depolarization-induced contraction of vascular smooth muscle via a Ca(2+) sensitization pathway. However, the role of Pyk2 in GI smooth muscle contraction is unclear. The spontaneous contraction of colonic smooth muscle was measured by using isometric force transducers. Protein and phosphorylation levels were determined by using western blotting. Pyk2 protein was expressed in colonic tissue, and spontaneous colonic contractions were inhibited by PF-431396, a Pyk2 inhibitor, in the presence of tetrodotoxin (TTX). In cultured colonic smooth muscle cells (CSMCs), PF-431396 decreased the levels of myosin light chain (MLC20) phosphorylated at Ser19 and ROCK2 protein expression, but myosin light chain kinase (MLCK) expression was not altered. However, Y-27632, a Rho kinase inhibitor, increased phosphorylation of Pyk2 at Tyr402 and concomitantly decreased ROCK2 levels; the expression of MLCK in CSMCs did not change. The expression of P(Tyr402)-Pyk2 and ROCK2 was increased when CSMCs were treated with Ach. Pyk2 is involved in the process of colonic smooth muscle contraction through the RhoA/ROCK pathway. These pathways may provide very important targets for investigating GI motility disorders.
- MeSH
- buněčné kultury MeSH
- fokální adhezní kinasa 2 antagonisté a inhibitory biosyntéza MeSH
- hladké svalstvo účinky léků metabolismus MeSH
- inhibitory enzymů farmakologie MeSH
- kinázy asociované s rho biosyntéza MeSH
- kolon účinky léků metabolismus MeSH
- myši inbrední ICR MeSH
- myši MeSH
- orgánové kultury - kultivační techniky MeSH
- rho proteiny vázající GTP biosyntéza MeSH
- signální transdukce účinky léků fyziologie MeSH
- svalová kontrakce účinky léků fyziologie MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Bcl-2/E1B-19K-interacting protein 3 (BNIP3) is a member of the apoptotic B-cell lymphoma-2 family that regulates cell death. Although BNIP3 targeted normally to the mitochondrial outer membrane by its transmembrane domain was originally considered to be essential for its pro-apoptotic activity, accumulating evidence has shown that BNIP3 is localized to endoplasmic reticulum at physiological conditions and that forced expression of BNIP3 can initiate cell death via multiple pathways depending on the subcellular compartment it targets. Targeting BNIP3 to endoplasmic reticulum has been shown to participate in cell death during endoplasmic reticulum stress. However, the molecular events responsible for BNIP3-induced cell death in the endoplasmic reticulum remain poorly understood. In the present study, the transmembrane domain of BNIP3 was replaced with a segment of cytochrome b5 that targets BNIP3 into endoplasmic reticulum, which induced cell death as effectively as its wild-type molecule in the SW480 cell line (colon carcinoma). Furthermore, a pan-caspase inhibitor, z-VAD-fmk, and PD150606, a specific calpain inhibitor, both significantly suppressed the endoplasmic reticulum-targeted BNIP3-induced cell death. These results suggest that endoplasmic reticulum-targeted BNIP3 induced a mixed mode of cell death requiring both caspases and calpains.
- MeSH
- apoptóza MeSH
- buněčná smrt * MeSH
- endoplazmatické retikulum * metabolismus MeSH
- kalpain * MeSH
- kaspasy * MeSH
- lidé MeSH
- membránové proteiny MeSH
- protoonkogenní proteiny MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
Actinomycete strain YIM PH20520, isolated from the rhizosphere soil sample of Panax notoginseng collected in Wenshang, Yunnan Province, China, exhibited antifungal activity against root-rot pathogens of the Panax notoginseng. The structures of bioactive molecules, isolated from the ethyl acetate extract of the fermentation broth of the strain, were identified as echinosporin (1) and 7-deoxyechinosporin (2) based on extensive spectroscopic analyses. 1 exhibited antifungal activity against four tested root-rot pathogens of Panax notoginseng include Fusarium oxysporum, Fusarium solani, Alternaria panax, and Phoma herbarum with the MIC value at 64, 64, 32, and 64 μg/mL, respectively. 2 exhibited antifungal activities against F. oxysporum, F. solani, A. panax, and P. herbarum with the MIC value at 128, 128, 64, and 128 μg/mL, respectively. Based on the phylogenetic analyses, the closest phylogenetic relative of strain YIM PH20520 is Amycolatopsis speibonae JS72T (97.69%), so strain YIM PH20520 was identified as Amycolatopsis strain. To the best of our knowledge, this is the first report of echinosporin antibiotics isolated from Amycolatopsis strain besides Streptomyces strain and their antifungal activity against four tested root-rot pathogens of the Panax notoginseng. The results provide a reliable evidence for the following related biosynthetic investigations on Amycolatopsis strain YIM PH20520 due to echinosporins antibiotics' unique tricyclic acetal-lactone structures.
- MeSH
- Actinobacteria chemie klasifikace genetika MeSH
- antifungální látky chemie izolace a purifikace farmakologie MeSH
- fylogeneze MeSH
- houby účinky léků MeSH
- kořeny rostlin mikrobiologie MeSH
- laktony chemie izolace a purifikace farmakologie MeSH
- mikrobiální testy citlivosti MeSH
- molekulární struktura MeSH
- nemoci rostlin mikrobiologie MeSH
- Panax notoginseng mikrobiologie MeSH
- rhizosféra MeSH
- RNA ribozomální 16S genetika MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Čína MeSH
Distension is a regular mechanical stimulus in gastrointestinal (GI) tract. This study was designed to investigate the effect of hypotonic stress on pacemaking activity and determine whether actin microfilament is involved in its mechanism in cultured murine intestinal interstitial cells of Cajal (ICCs) by using whole-cell patch-clamp and calcium imaging techniques. Hypotonic stress induced sustained inward holding current from the baseline to -650+/-110 pA and significantly decreased amplitudes of pacemaker current. Hypotonic stress increased the intensity of basal fluorescence ratio (F/F0) from baseline to 1.09+/-0.03 and significantly increased Ca(2+) oscillation amplitude. Cytochalasin-B (20 microM), a disruptor of actin microfilaments, significantly suppressed the amplitudes of pacemaker currents and calcium oscillations, respectively. Cytochalasin-B also blocked hypotonic stress-induced sustained inward holding current and hypotonic stress-induced increase of calcium oscillations. Phalloidin (20 microM), a stabilizer of actin microfilaments, significantly enhanced the amplitudes of pacemaker currents and calcium oscillations, respectively. Despite the presence of phalloidin, hypotonic stress was still able to induce an inward holding current and increased the basal fluorescence intensity. These results suggest that hypotonic stress induces sustained inward holding current via actin microfilaments and the process is mediated by alteration of intracellular basal calcium concentration and calcium oscillation in cultured intestinal ICCs.
- MeSH
- biologické hodiny fyziologie MeSH
- buněčný převod mechanických signálů fyziologie MeSH
- gastrointestinální motilita fyziologie MeSH
- kultivované buňky MeSH
- mikrofilamenta metabolismus MeSH
- myši inbrední BALB C MeSH
- myši MeSH
- osmotický tlak fyziologie MeSH
- telocyty fyziologie MeSH
- vápníková signalizace fyziologie MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
