• MeSH
• terpeny analýza   MeSH
• vystavení vlivu životního prostředí MeSH
• znečištění ovzduší MeSH

• Konspekt
• Biochemie. Molekulární biologie. Biofyzika
• NLK Obory
• biochemie
• endokrinologie

• MeSH
• terpeny * MeSH
• výzkum * MeSH
• Publikační typ
• kongresy MeSH
• úvodníky MeSH

• Konspekt
• Biochemie. Molekulární biologie. Biofyzika
• NLK Obory
• biochemie
• endokrinologie

• MeSH
• lidé MeSH
• terpeny * chemie   metabolismus   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• kongresy MeSH
• úvodníky MeSH

Fluxes of CO2 and isoprenoids were measured for the first time in Stipa tenacissima L (alfa grass), a perennial tussock grass dominant in the driest areas of Europe. In addition, we studied how those fluxes were influenced by environmental conditions, leaf ontogeny and UV radiation and compared emission rates in two contrasting seasons: summer when plants are mostly inactive and autumn, the growing season in this region. Leaf ontogeny significantly affected both photosynthesis and isoprenoids emission. Isoprene emission was positively correlated with photosynthesis, although a low isoprene emission was detected in brown leaves with a net carbon loss. Moreover, leaves with a significant lower photosynthesis emitted only monoterpenes, while at higher photosynthetic rates also isoprene was produced. Ambient UV radiation uncoupled photosynthesis and isoprene emission. It is speculated that alfa grass represent an exception from the general rules governing plant isoprenoid emitters.

• MeSH
• butadieny MeSH
• fotosyntéza fyziologie   MeSH
• hemiterpeny MeSH
• látky znečišťující vzduch analýza   MeSH
• lipnicovité metabolismus   MeSH
• listy rostlin MeSH
• monitorování životního prostředí MeSH
• monoterpeny MeSH
• oxid uhličitý MeSH
• pentany MeSH
• rostliny MeSH
• terpeny analýza   MeSH
• uhlík MeSH
• ultrafialové záření * MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Evropa MeSH

BACKGROUND: Statin treatment of hypercholesterolemia is accompanied also with depletion of the mevalonate intermediates, including farnesyl pyrophosphate (FPP) and geranylgeranyl pyrophosphate (GGPP) necessary for proper function of small GTPases. These include Ras proteins, prevalently mutated in pancreatic cancer. In our study, we evaluated the effect of three key intermediates of the mevalonate pathway on GFP-K-Ras protein localization and the gene expression profile in pancreatic cancer cells after exposure to individual statins. METHODS: These effects were tested on MiaPaCa-2 human pancreatic cancer cells carrying a K-Ras activating mutation (G12C) after exposure to individual statins (20 μM). The effect of statins (atorvastatin, lovastatin, simvastatin, fluvastatin, cerivastatin, rosuvastatin, and pitavastatin) and mevalonate intermediates on GFP-K-Ras protein translocation was analyzed using fluorescence microscopy. The changes in gene expression induced in MiaPaCa-2 cells treated with simvastatin, FPP, GGPP, and their combinations with simvastatin were examined by whole genome DNA microarray analysis. RESULTS: All tested statins efficiently inhibited K-Ras protein trafficking from cytoplasm to the cell membrane of the MiaPaCa-2 cells. The inhibitory effect of statins on GFP-K-Ras protein trafficking was partially prevented by addition of any of the mevalonate pathway's intermediates tested. Expressions of genes involved in metabolic and signaling pathways modulated by simvastatin treatment was normalized by the concurrent addition of FPP or GGPP. K-Ras protein trafficking within the pancreatic cancer cells is effectively inhibited by the majority of statins; the inhibition is eliminated by isoprenoid intermediates of the mevalonate pathway. CONCLUSIONS: Our data indicate that the anticancer effects of statins observed in numerous studies to a large extent are mediated through isoprenoid intermediates of the mevalonate pathway, as they influence expression of genes involved in multiple intracellular pathways.

• MeSH
• anticholesteremika farmakologie   MeSH
• atorvastatin farmakologie   MeSH
• beta-buňky účinky léků   metabolismus   patologie   MeSH
• indoly farmakologie   MeSH
• kyselina mevalonová analogy a deriváty   farmakologie   MeSH
• kyseliny mastné mononenasycené farmakologie   MeSH
• lidé MeSH
• lovastatin farmakologie   MeSH
• mikročipová analýza MeSH
• mutace MeSH
• nádorové buněčné linie MeSH
• polyisoprenylfosfáty farmakologie   MeSH
• prenylace proteinů MeSH
• protoonkogenní proteiny p21(ras) genetika   metabolismus   MeSH
• regulace genové exprese MeSH
• rekombinantní fúzní proteiny genetika   metabolismus   MeSH
• seskviterpeny farmakologie   MeSH
• signální transdukce MeSH
• simvastatin farmakologie   MeSH
• stanovení celkové genové exprese MeSH
• transport proteinů účinky léků   MeSH
• zelené fluorescenční proteiny genetika   metabolismus   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH

MAIN CONCLUSION: The present review summarizes current knowledge of the biosynthesis and biological importance of isoprenoid-derived plant signaling compounds. Cellular organisms use chemical signals for intercellular communication to coordinate their growth, development, and responses to environmental cues. The skeletons of majority of plant signaling molecules, mediators of plant intercellular 'broadcasting', are built from C5 units of isoprene and therefore belong to a huge and diverse group of natural substances called isoprenoids (terpenoids). They fill many important roles in nature. This review summarizes current knowledge of the biosynthesis and biological importance of a group of isoprenoid-derived plant signaling compounds.

• MeSH
• brassinosteroidy biosyntéza   MeSH
• cytokininy biosyntéza   MeSH
• gibereliny biosyntéza   MeSH
• kyselina abscisová biosyntéza   MeSH
• metabolické sítě a dráhy MeSH
• regulátory růstu rostlin biosyntéza   MeSH
• rostliny metabolismus   MeSH
• signální transdukce * MeSH
• terpeny metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH

• MeSH
• lidé MeSH
• terpeny chemie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• portréty MeSH
• úvodníky MeSH

Males of the closely related species Bombus terrestris and Bombus lucorum attract conspecific females by completely different marking pheromones. MP of B. terrestris and B. lucorum pheromones contain mainly isoprenoid (ISP) compounds and fatty acid derivatives, respectively. Here, we studied the regulation of ISP biosynthesis in both bumblebees. RNA-seq and qRT-PCR analyses indicated that acetoacetyl-CoA thiolase (AACT), 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR), and farnesyl diphosphate synthase (FPPS) transcripts are abundant in the B. terrestris labial gland. Maximal abundance of these transcripts correlated well with AACT enzymatic activity detected in the LG extracts. In contrast, transcript abundances of AACT, HMGR, and FPPS in B. lucorum were low, and AACT activity was not detected in LGs. These results suggest that transcriptional regulation plays a key role in the control of ISP biosynthetic gene expression and ISP pheromone biosynthesis in bumblebee males.

• MeSH
• feromony biosyntéza   chemie   MeSH
• hmyzí proteiny genetika   metabolismus   MeSH
• kontigové mapování MeSH
• regulace genové exprese MeSH
• RNA chemie   izolace a purifikace   metabolismus   MeSH
• sekvenční analýza RNA MeSH
• terpeny chemie   metabolismus   MeSH
• včely chemie   genetika   metabolismus   MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH