Kinetic analysis
Dotaz
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427 s. : il.
- MeSH
- dospělí MeSH
- glukózový toleranční test metody MeSH
- krevní glukóza analýza MeSH
- lidé MeSH
- porucha glukózové tolerance diagnóza farmakokinetika MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- srovnávací studie MeSH
The objective of this study was to devise a method of kinetic analysis of the ground reaction force that enables the durations and magnitudes of forces acting during the individual phases of ski turns to be described exactly. The method is based on a theoretical analysis of physical forces acting during the ski turn. Two elementary phases were defined: (1) preparing to turn (initiation) and (2) actual turning, during which the center of gravity of the skier-ski system moves along a curvilinear trajectory (steering). The starting point of the turn analysis is a dynamometric record of the resultant acting ground reaction force applied perpendicularly on the ski surface. The method was applied to six expert skiers. They completed a slalom course comprising five gates arranged on the fall line of a 26° slope at a competition speed using symmetrical carving turns (30 evaluated turns). A dynamometric measurement system was placed on the carving skis (168 cm long, radius 16 m, data were recorded at 100 Hz). MATLAB procedures were used to evaluate eight variables during each turn: five time variables and three force variables. Comparison of the turn analysis results between individuals showed that the method is useful for answering various research questions associated with ski turns.
- MeSH
- časové faktory MeSH
- dospělí MeSH
- kinetika MeSH
- lidé MeSH
- lyžování zranění fyziologie MeSH
- neparametrická statistika MeSH
- statistika jako téma MeSH
- svalová síla - dynamometr MeSH
- teoretické modely MeSH
- točivý moment MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Aldehyde dehydrogenases (ALDHs) constitute a superfamily of NAD(P)+-dependent enzymes, which detoxify aldehydes produced in various metabolic pathways to the corresponding carboxylic acids. Among the 19 human ALDHs, the cytosolic ALDH9A1 has so far never been fully enzymatically characterized and its structure is still unknown. Here, we report complete molecular and kinetic properties of human ALDH9A1 as well as three crystal forms at 2.3, 2.9, and 2.5 Å resolution. We show that ALDH9A1 exhibits wide substrate specificity to aminoaldehydes, aliphatic and aromatic aldehydes with a clear preference for γ-trimethylaminobutyraldehyde (TMABAL). The structure of ALDH9A1 reveals that the enzyme assembles as a tetramer. Each ALDH monomer displays a typical ALDHs fold composed of an oligomerization domain, a coenzyme domain, a catalytic domain, and an inter-domain linker highly conserved in amino-acid sequence and folding. Nonetheless, structural comparison reveals a position and a fold of the inter-domain linker of ALDH9A1 never observed in any other ALDH so far. This unique difference is not compatible with the presence of a bound substrate and a large conformational rearrangement of the linker up to 30 Å has to occur to allow the access of the substrate channel. Moreover, the αβE region consisting of an α-helix and a β-strand of the coenzyme domain at the dimer interface are disordered, likely due to the loss of interactions with the inter-domain linker, which leads to incomplete β-nicotinamide adenine dinucleotide (NAD+) binding pocket.
- MeSH
- aldehyddehydrogenasa antagonisté a inhibitory chemie genetika ultrastruktura MeSH
- katalytická doména genetika MeSH
- kinetika MeSH
- konformace proteinů * MeSH
- krystalografie rentgenová MeSH
- lidé MeSH
- NAD genetika MeSH
- sekundární struktura proteinů MeSH
- sekvence aminokyselin genetika MeSH
- substrátová specifita genetika MeSH
- vazebná místa genetika MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
... Strube Anthony Delitto -- 11 Observational Gait Analysis, 112 Francine Malouin -- 12 Foot Fall Measurement ... ... Analysis in Human Gait, 223 Rami Seliktar Lin Bo -- 18 Measurement of Kinetic Parameters Technology, ... ... Barnes Necip Berme -- 19 A Review of Kinetic Parameters in Human Walking, 252 David A. ... ... Rodgers -- 27 Gait Analysis as an Outcome Measure After Anterior Cruciate Ligament Injury, 376 -- Lynn ... ... McClay -- 30 Gait Analysis in Stroke and Head Injury, 412 -- Alberto Esquenazi Barbra Hirai -- 31 Gait ...
XV, 471 s. : il. ; 26 cm
- MeSH
- buněčné struktury MeSH
- iontové kanály MeSH
- kardiomyocyty cytologie MeSH
- krysa rodu rattus MeSH
- spřažení excitace a kontrakce MeSH
- srdeční elektrofyziologie MeSH
- srdeční komory MeSH
- statistika jako téma MeSH
- teoretické modely MeSH
- vápníkové kanály MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- zvířata MeSH
This study describes the meta-analysis and kinetic modelling of gene expression control by sigma factor SigA of Bacillus subtilis during germination and outgrowth based on microarray data from 14 time points. The analysis computationally models the direct interaction among SigA, SigA-controlled sigma factor genes (sigM, sigH, sigD, sigX), and their target genes. Of the >800 known genes in the SigA regulon, as extracted from databases, 311 genes were analysed, and 190 were confirmed by the kinetic model as being controlled by SigA. For the remaining genes, alternative regulators satisfying kinetic constraints were suggested. The kinetic analysis suggested another 214 genes as potential SigA targets. The modelling was able to (i) create a particular SigA-controlled gene expression network that is active under the conditions for which the expression time series was obtained, and where SigA is the dominant regulator, (ii) suggest new potential SigA target genes, and (iii) find other possible regulators of a given gene or suggest a new mechanism of its control by identifying a matching profile of unknown regulator(s). Selected predicted regulatory interactions were experimentally tested, thus validating the model.
- MeSH
- Bacillus subtilis genetika MeSH
- bakteriální proteiny genetika MeSH
- genetická transkripce genetika MeSH
- genové regulační sítě genetika MeSH
- kinetika MeSH
- regulace genové exprese u bakterií genetika MeSH
- sigma faktor genetika MeSH
- spory bakteriální genetika MeSH
- transkripční faktory genetika MeSH
- Publikační typ
- časopisecké články MeSH
- metaanalýza MeSH
- práce podpořená grantem MeSH
