PROGESTERONE/metabolism Dotaz Zobrazit nápovědu

Přesná shoda Sémantické

Steroids constitute one of the most important groups of compounds of regulatory properties both in the animal and plant kingdom. In plants, steroids such as brassinosteroids or progesterone, by binding to protein receptors in cell membranes, regulate growth and initiate processes leading to increased tolerance to stress conditions. Due to their structural similarities to sterols, these steroids may also directly interact with cellular membranes. Our aim was to determine the changes of the structural parameters of lipid membranes under the influence of hydrophobic steroid compounds, i.e., 24-epibrassinolide (EBR) and its precursor-24-epicastasterone (ECS) and progesterone (PRO). Lipids were isolated from wheat seedlings with different tolerances to frost, grown at low temperatures (5 °C) for 1.5 and 3 weeks (acclimation process). Control plants were cultured continuously at 20 °C. From galactolipids and phospholipids, the main polar lipid fractions, the monolayers were formed, using a technique of Langmuir trough. EBR and ECS were introduced into monolayers, together with lipids, whereas the PRO was dissolved in the aqueous sub-phase upon which the monolayers were spread. Measurements performed at 25 °C and 10 °C showed a significant action of the tested compounds on the physicochemical properties of the monolayers. EBR and PRO increased the area per lipid molecule in monolayers, resulting in formation of more flexible surface structures while the presence of the ECS induced the opposite effect. The influence of the polarity of lipids and steroids on the interactions in the monolayer was discussed. Lipids extracted from the membranes of wheat with the most tolerance to frost were characterized by the highest fatty acid unsaturation and steroids had a relatively weak effect on the parameters of the structure of their monolayers.

Článek

Důsledky působení ovariálních hormonů na tukový metabolismus : Z přednášk.večera 3.inter.klin. 1.LF UK v Praze dne 10.2.1992

Marek, J
Autor Marek, J

Časopis lékařů českých. 1992 ; Roč. 131 (č. 12) : S. 384.

ISSN 0008-7335
Medvik
Zdroj

MeSH
estrogeny farmakologie fyziologie terapeutické užití MeSH
kongenery progesteronu farmakologie fyziologie škodlivé účinky MeSH
lidé MeSH
lipidy MeSH
metabolismus lipidů MeSH
Check Tag
lidé MeSH
ženské pohlaví MeSH

Článek online

Estrogen-progestinová substituční terapie v postmenopauze s antiandrogenním progestinem cyproteronacetátem [Oestrogen-progestin substitution therapy during the postmenopausal period with antiandrogenic progestin cyproterone acetate]

Presl, J
Autor Presl, J

Česká gynekologie. 1995 ; Roč. 60 (č. 2) : s. 98-101.

ISSN 1210-7832
Medvik
Zdroj

MeSH
antagonisté androgenů MeSH
dospělí MeSH
estrogenní substituční terapie metody MeSH
kombinovaná farmakoterapie MeSH
lidé MeSH
lipidy MeSH
menopauza účinky léků MeSH
metabolismus lipidů MeSH
progesteron analogy a deriváty MeSH
Check Tag
dospělí MeSH
lidé MeSH
ženské pohlaví MeSH

Článek online

Influence of ligand binding on structure and thermostability of human α1-acid glycoprotein

JMR. Journal of molecular recognition. 2016 ; 29 (2) : 70-9. [pub] 20150924

J Mol Recognit
ISSN 1099-1352
Medvik
Zdroj

Ligand binding of neutral progesterone, basic propranolol, and acidic warfarin to human α1-acid glycoprotein (AGP) was investigated by Raman spectroscopy. The binding itself is characterized by a uniform conformational shift in which a tryptophan residue is involved. Slight differences corresponding to different contacts of the individual ligands inside the β-barrel are described. Results are compared with in silico ligand docking into the available crystal structure of deglycosylated AGP using quantum/molecular mechanics. Calculated binding energies are -18.2, -14.5, and -11.5 kcal/mol for warfarin, propranolol, and progesterone, respectively. These calculations are consistent with Raman difference spectroscopy; nevertheless, minor discrepancies in the precise positions of the ligands point to structural differences between deglycosylated and native AGP. Thermal dynamics of AGP with/without bounded warfarin was followed by Raman spectroscopy in a temperature range of 10-95 °C and analyzed by principal component analysis. With increasing temperature, a slight decrease of α-helical content is observed that coincides with an increase in β-sheet content. Above 45 °C, also β-strands tend to unfold, and the observed decrease in β-sheet coincides with an increase of β-turns accompanied by a conformational shift of the nearby disulfide bridge from high-energy trans-gauche-trans to more relaxed gauche-gauche-trans. This major rearrangement in the vicinity of the bridge is not only characterized by unfolding of the β-sheet but also by subsequent ligand release. Hereby, ligand binding alters the protein dynamics, and the more rigid protein-ligand complex shows an improved thermal stability, a finding that contributes to the reported chaperone-like function of AGP.

Výzkumná zpráva

Zdokonalení molekulární diagnostiky kongenitální adrenální hyperplázie (CAH)

Pouchlá, Slávka
Autor Autorita
Fakultní nemocnice (Brno, Česko)
Autor Autorita

Praha : Iga MZ ČR, 2010

Závěrečná zpráva o řešení grantu Interní grantové agentury MZ ČR

Přeruš. str. : il., tab. ; 30 cm

Předmětem projektu je zdokonalení současného stavu DNA diagnostiky kongenitální adrenální hyperplasie (CAH) v České republice. Studiem genů CYP21 a CYP11B1 s využitím technik jako MLPA, LR PCR, gene walking, DHPLC a sekvenování chceme zvýšit efektivitu mutační analýzy CAH v rodinách s výskytem tohoto život ohrožujícího metabolického onemocnění.; The aim of the project is to improve present DNA diagnostics of congenital adrenal hyperplasia (CAH) in the Czech Republic. By study of CYP21 and CYP21P genes with using of techniques such as MLPA, LR PCR, gene walking, DHPLC and sequencing we want to increase the effectiveness of mutation analysis of CAH in affected families.