Mutations extended the host range of the polyvalent bacteriophage 812 of the family Myoviridae in up to 95 % of Staphylococcus aureus strains and 43 % of strains of different coagulase-positive and -negative Staphylococcus species. Mutational changes in the genome of several host-range mutants of phage 812 were identified. Host-range mutant 812F1 harbors a deletion in endolysin gene that arose together with intron excision. Four mutants (812i, 812b, 812p, 812F3) harbor deletion in the structural gene orf8 that results from a genome rearrangement associated with intron insertion. This rearrangement was also detected in the genome of the closely related phages U16 and phi131. Another intron was discovered in the recA812 gene in these four mutants. An insertion was found in a non-coding region of the restriction fragment PstI-O of three mutants (812b, 812F3, 812g) and phages U16 and phi131. The above results contribute to the explanation of genetic factors affecting the host range of polyvalent staphylococcal bacteriophages.
- MeSH
- Bacteriophages genetics immunology MeSH
- Research Support as Topic MeSH
- Myoviridae genetics immunology MeSH
- Reverse Transcriptase Polymerase Chain Reaction methods utilization MeSH
- Polymorphism, Restriction Fragment Length MeSH
- S-Adenosylmethionine genetics chemistry immunology MeSH
- Sequence Analysis, DNA methods utilization MeSH
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization methods utilization MeSH
- Staphylococcus Phages genetics immunology MeSH
Phage 812 is a polyvalent phage with a very broad host range in the genus Staphylococcus, which makes it a suitable candidate for phage therapy of staphylococcal infections. This proteomic study, combining the results of both 1-DE and 2-DE followed by PMF, led to the identification of 24 virion proteins. Twenty new proteins, not yet identified by proteome analysis of closely related staphylococcal phages K and G1 were identified using this approach. Fifteen proteins were assigned unambiguously to the head-tail genome module; the remaining nine proteins are encoded by genes of the left or right arms of the phage genome. As expected, the most abundant proteins in the electrophoretic patterns are the major capsid protein, the major tail sheath protein and proteins identical to ORF 50 and ORF 95 of phage K, although their function is only putative. Identification of these 20 new proteins contributes substantially to a detailed characterization of phage virions, knowledge of which is necessary for rational phage therapy.
Představujeme kazuistiku mladého muže s atopickým ekzémem/dermatitidou a polyvalentní potravinovou alergií, klinicky projevenou převážně zhoršováním průběhu atopické dermatitidy po většině potravin. Multiplexovou komponentovou diagnostikou (metodou ALEX –Allergy Explorer)jsme vyšetřili specifické IgE a klasická celková léčba byla doplněna o kombinaci prebiotika s enterosorbentem. Zavedení této léčby vedlo k velmi dobrému okamžitému klinickému efektu.
We present acase report of ayoung man with atopic eczema/dermatitis and polyvalent food allergy, clinically manifested predominantly with worsening of the course of atopic dermatitis, following aconsumption of most food. We used the ALEX (Allergy Explorer) multiplex method to analyse specific IgE, and the usual overall treatment was supplemented with administration of prebiotics and enterosorbents. Introduction of this treatment resulted in very good and immediate clinical effect.
- Keywords
- Atopona,
- MeSH
- Dermatitis, Atopic drug therapy immunology MeSH
- Humans MeSH
- Food Hypersensitivity * drug therapy immunology MeSH
- Prebiotics MeSH
- Treatment Outcome MeSH
- Check Tag
- Humans MeSH
- Male MeSH
- Publication type
- Case Reports MeSH
- Keywords
- TAVEGYL (KRKA),
- MeSH
- Adult MeSH
- Vasculitis, Leukocytoclastic, Cutaneous diagnosis etiology MeSH
- Skin Tests MeSH
- Pharmaceutical Preparations MeSH
- Humans MeSH
- Drug-Related Side Effects and Adverse Reactions MeSH
- Check Tag
- Adult MeSH
- Humans MeSH
- Female MeSH
- Publication type
- Case Reports MeSH
[1st ed.] vii, 88 s. : il.
- MeSH
- Dermatitis, Allergic Contact etiology MeSH
- Dermatologic Agents adverse effects MeSH
- Humans MeSH
- Check Tag
- Humans MeSH
- Female MeSH
- Publication type
- Case Reports MeSH
