Tato kazuistika popisuje účinek léčby přípravkem Ecosin (registrovaná forma Pythium oligandrum pro zvířata) u kotěte britské kočky, u kterého byla klinicky a laboratorně diagnostikována dermatofytóza. Jako původce onemocnění bylo diagnostikováno Microsporum canis. Kotěti byla opakovaně aplikována koupel v přípravku Ecosin, který obsahuje živé kultury mykoparazitické houby Pythium oligandrum. Byly aplikovány tři celotělové koupele s týdenním odstupem a lokální aplikací přípravku, pokaždé druhý den po koupeli. Klinické vyšetření kotěte týden od ukončení aplikací již neprokázalo žádné příznaky dermatomykózy, negativní byly také kontrolní kultivace a trichoskopické vyšetření. Léčba přípravkem obsahujícím P. oligandrum byla bez jakýchkoli komplikací, bez nežádoucích i vedlejších účinků.

Presented case report describes therapeutical effect of biologic preparation Ecosin (registered form of mycoparasitic fungus Pythium oligandrum for animals) in a kitten of British shorthair cat, in which dermatophythosis has been diagnosed by means of clinical and laboratory examinations. Microsporum canis was identified as causal organism. The kitten was repeatedly treated by Ecosin in form of bath. Overall three baths were applied in a week interval and local administration of Ecosin was performed next day after each bath. No clinical symptoms were presented and both mycologic cultivation and trichoscopic examinations were negative after this medication. No adverse effect was recorded during and after treatment of the kitten by Ecosin.

• MeSH
• Dermatitis * diagnosis   therapy   veterinary   MeSH
• Clinical Laboratory Techniques MeSH
• Cats MeSH
• Microsporum * MeSH
• Mycoses * therapy   MeSH
• Cat Diseases MeSH
• Pythium * MeSH
• Zoonoses MeSH
• Animals MeSH
• Check Tag
• Cats MeSH
• Animals MeSH
• Publication type
• Case Reports MeSH

Mycoparasitism, the ability to parasitize fungal hosts, is an important mechanism useful in plant biocontrol. Pythium species shows the ability of mycoparasitism on pathogenic fungi invading not only plant hosts. Due to this fact, the oomycete genus Pythium has a potential to be used in the development of biocontrol agents for the treatment of fungal infections.

• MeSH
• Parasites MeSH
• Pythium MeSH

• MeSH
• Mycoses therapy   MeSH
• Pythium MeSH

Pythium oligandrum is a soil born free living oomycete able to parasitize fungi and oomycetes prey, including important plant and animals pathogens. Pythium oligandrum can colonize endophytically the root tissues of diverse plants where it induces plant defenses. Here we report the first long-read genome sequencing of a P. oligandrum strain sequenced by PacBio technology. Sequencing of genomic DNA loaded onto six SMRT cells permitted the acquisition of 913,728 total reads resulting in 112X genome coverage. The assembly and polishing of the genome sequence yielded180 contigs (N50 = 1.3 Mb; L50 = 12). The size of the genome assembly is 41.9 Mb with a longest contig of 2.7 Mb and 15,007 predicted protein-coding genes among which 95.25% were supported by RNAseq data, thus constituting a new Pythium genome reference. This data will facilitate genomic comparisons of Pythium species that are commensal, beneficial or pathogenic on plant, or parasitic on fungi and oomycete to identify key genetic determinants underpinning their diverse lifestyles. In addition comparison with plant pathogenic or zoopathogenic species will illuminate genomic adaptations for pathogenesis toward widely diverse hosts.

• MeSH
• Beta vulgaris parasitology   MeSH
• Genome MeSH
• Proteome MeSH
• Pythium genetics   metabolism   MeSH
• Rhizosphere MeSH
• RNA-Seq MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH

AIMS: Screening of bacterial flora for strains producing metabolites with inhibitory effects on the human pathogenic oomycete Pythium insidiosum. Separation and characterization of extracts from Pseudomonas stutzeri with anti-Pythium inhibitory activity. Search for genes with anti-Pythium effect within the genome of P. stutzeri. METHODS: A total of 88 bacterial strains were isolated from water resources in northeastern Thailand. Two screening methods were used to establish their inhibitory effects on P. insidiosum. One strain, P. stutzeri ST1302 was randomly chosen, and the extract with anti-P. insidiosum activity was fractionated and subfractionated using liquid column chromatography and purified by thin layer chromatography. The chemical structure of purified fractions was determined by Fourier transform infrared spectroscopy, nuclear magnetic resonance and mass spectrometry. Further, search for genes involved in the anti-Pythium activity (phenazine-1-carboxylic acid, 2,4-diacetylphloroglucinol, pyoluteorin and pyrrolnitrin) was undertaken in this P. stutzeri strain using primers described in the literature. RESULTS: Anti-P. insidiosum activity was detected in 16 isolates (18.2%). In P. stutzeri ST1302, a subfraction labeled PYK7 exhibited strong activity against this oomycete. It was assigned to the diketopiperazines as cyclo(D-Pro-L-Val). In the search for genes, one gene region was successfully amplified. This corresponded to pyrrolnitrin. The results suggest the possibility of using the related metabolites against P. insidiosum. This is the first report on the inhibitory effects of P. stutzeri against this oomycete. The results may contribute to the development of antimicrobial drugs/probiotics against pythiosis.

• MeSH
• Diketopiperazines pharmacology   MeSH
• Genome, Bacterial MeSH
• Microbial Sensitivity Tests MeSH
• Pseudomonas stutzeri chemistry   genetics   isolation & purification   MeSH
• Pyrrolnitrin pharmacology   MeSH
• Pythiosis drug therapy   microbiology   MeSH
• Pythium drug effects   MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Geographicals
• Thailand MeSH

Objectives Dermatophytosis, commonly known as ringworm, is a superficial fungal skin disease and zoonosis. Pythium oligandrum is a micromycete with mycoparasitic properties that is used in agriculture to control fungal infections on plants. Formulations containing P oligandrum were also developed for the treatment of dermatophytoses, but only a small number of case studies have been published. In order to document the process in simplified conditions in vitro, we investigated the effectiveness of P oligandrum against three pathogenic dermatophytes common in domestic animals. Methods Cultures of the pathogens grown on nutrient media and experimentally infected cat hair were treated with P oligandrum preparations in therapeutic concentration and the changes were documented by microscopic videos and scanning electron microscopy. Results There was strong mycoparasitic activity of P oligandrum against Microsporum canis, Microsporum gypseum and Trichophyton mentagrophytes. Conclusions and relevance P oligandrum was demonstrated to be effective against three common causes of dermatophytosis in vitro.

• MeSH
• Antifungal Agents pharmacology   MeSH
• Cats MeSH
• Microsporum drug effects   MeSH
• Cat Diseases drug therapy   microbiology   MeSH
• Parasitic Sensitivity Tests veterinary   MeSH
• Pythium * MeSH
• Tinea drug therapy   microbiology   veterinary   MeSH
• Trichophyton drug effects   MeSH
• Hair microbiology   MeSH
• Treatment Outcome MeSH
• Animals MeSH
• Check Tag
• Cats MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH

Transcription factors (TFs) fine-tune the host defense transcriptome in response to pathogen invasions. No information is available on Zingiber zerumbet (Zz) TFs involved in defense response against Pythium myriotylum. Here, we provide a global identification, characterization, and temporal expression profiling of Zz TFs following an incompatible interaction with P. myriotylum using a transcriptome sequencing approach. We identified a total of 903 TFs belonging to 96 families based on their conserved domains. Evolutionary analysis clustered the Zz TFs according to their phylogenetic affinity, providing glimpses of their functional diversities. High throughput expression array analysis highlighted a complex interplay between activating and repressing transcription factors in fine-tuning Zz defense response against P. myriotylum. The high differential modulation of TFs involved in cell wall fortification, lignin biosynthesis, and SA/JA hormone crosstalk allows us to envisage that this mechanism plays a central role in restricting P. myriotylum proliferation in Zz. This study lays a solid foundation and provides valuable resources for the investigation of the evolutionary history and biological functions of Zz TF genes involved in defense response.

• MeSH
• Stress, Physiological MeSH
• Plant Immunity * MeSH
• Evolution, Molecular MeSH
• Pythium pathogenicity   MeSH
• Response Elements MeSH
• Plant Proteins genetics   metabolism   MeSH
• Transcription Factors genetics   metabolism   MeSH
• Transcriptome * MeSH
• Zingiberaceae genetics   immunology   microbiology   MeSH
• Publication type
• Journal Article MeSH

• MeSH
• Biopharmaceutics MeSH
• Biological Therapy MeSH
• Dermatomycoses etiology   prevention & control   therapy   MeSH
• Lower Extremity MeSH
• Humans MeSH
• Pythium MeSH
• Check Tag
• Humans MeSH

• MeSH
• Bacterial Load MeSH
• Biofilms MeSH
• Lower Extremity MeSH
• Wound Healing * drug effects   MeSH
• Pharmaceutical Solutions therapeutic use   MeSH
• Humans MeSH
• Bandages, Hydrocolloid MeSH
• Pythium * MeSH
• Wounds and Injuries microbiology   MeSH
• Treatment Outcome MeSH
• Check Tag
• Humans MeSH
• Male MeSH
• Female MeSH
• Publication type
• Meeting Abstract MeSH