biocompatible 3D printing
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Skeletální traumatologie prošla ve světě v poslední dekádě revolučními změnami v souvislosti s rozvojem technologií 3D tisku. Tento přehledový článek má za cíl přinést ucelený přehled o tom, jakým způsobem 3D tisk transformuje oblast léčby zlomenin a otevírá nové možnosti v řešení komplexních zlomenin. Využití 3D tisku v medicíně nabízí nový rozměr v přesnosti a individualizaci léčby, umožňuje vytváření personalizovaných chirurgických šablon, individualizovaných implantátů a nástrojů. Rozvoj 3D tisku je úzce propojen s dalšími technologickými pokroky, jako jsou metody augmentované reality, což představuje významný krok vpřed ve vizualizaci a plánování chirurgických zákroků. Přestože 3D aditivní technologie nabízí řadu výhod, její začlenění do běžné klinické praxe stále čelí mnoha výzvám. Tento článek rovněž zkoumá historii a vývoj technologie 3D tisku, materiály používané v medicíně, předoperační plánování, tvorbu chirurgických cíličů, výrobu pacient specifických implantátů a integraci této technologie spolu s metodami augmentované reality ve skeletální chirurgii, přičemž zdůrazňuje technické, logistické a etické výzvy při implementaci této technologie do chirurgické praxe.
The field of skeletal traumatology has undergone revolutionary changes worldwide over the last decade with the development of 3D printing technologies. This review aims to provide a comprehensive overview of how 3D printing is transforming fracture treatment and opening up new possibilities in the management of complex fractures. The use of 3D printing in medicine offers a new dimension in precision and customisation of treatment, enabling the creation of personalised surgical templates, individualised implants and tools. The development of 3D printing is closely linked to other technological advances, such as augmented reality methods, which represent a significant step forward in the visualisation and planning of surgical procedures. Although 3D printing offers many advantages, its integration into routine clinical practice still faces many challenges. This article examines the history and development of 3D printing technology, materials used in medicine, preoperative planning, the creation of surgical guides, the fabrication of patient-specific implants, and the integration of 3D printing and augmented reality in skeletal surgery, highlighting the technical, logistical, and ethical challenges of implementing this technology in surgical practice.
- MeSH
- 3D tisk * trendy MeSH
- biokompatibilní materiály MeSH
- design s pomocí počítače MeSH
- fraktury kostí * terapie MeSH
- kostní náhrady MeSH
- lidé MeSH
- traumatologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- přehledy MeSH
3D printing technology has developed rapidly in the last decade. In addition to its common use in industrial field, 3D printing is gaining importance in medicine and pharmacy. This review provides information on the use of 3D printing in organ and tissue formation. It describes examples currently presented in this field of research, and the specific use of artificial tissues in clinical practice. The article also deals with the modelling of specific implants and prostheses tailored to the individual patient. Moreover, for complicated surgeries, 3D printing is used to create models which are used to practise and select the correct surgical procedure. The use of 3D printers in the production of medicines and specific drug delivery systems is also discussed. Due to the development of 3D bioprinting in tissue engineering, the second part of the article is devoted to a detailed overview of biocompatible materials and bioinks, accompanied by specific examples of their use.
3D printing seems to be the technology of the future for the preparation of metallic implants. For such applications, corrosion behaviour is pivotal. However, little is published on this topic and with inconsistent results. Therefore, we carried out a complex study in which we compared two techniques of the 3D printing technology - selective laser melting and electron beam melting. The corrosion behaviour was studied in physiological solution by standard electrochemical techniques and susceptibility to localised corrosion was estimated too. All samples showed typical passive behaviour. Localised corrosion was shown to be possible on the original as-printed surfaces. Corrosion experiments were repeated tree times. To reveal possible negative effects of 3D printing on cytocompatibility, direct in vitro tests were performed with U-2 OS cells. The cells showed good viability and proliferation, but their growth was impeded by surface unevenness. Our results suggest that both techniques are suitable for implants production. Statistical evaluation was performed by ANOVA followed by Tukey's test.
- MeSH
- 3D tisk * MeSH
- koroze MeSH
- lidé MeSH
- nádorové buněčné linie MeSH
- proliferace buněk účinky léků MeSH
- testování materiálů * MeSH
- titan * chemie farmakologie MeSH
- viabilita buněk účinky léků MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
Článok predstavuje a hodnotí prelomové technologické postupy v ortodoncii: skutočný a pseudo 3D záznam, ich spracovanie a vizualizácie. Popisuje postup od celohlavového záznamu CBCT iCAT cez export do stereolitografického formátu (STL) až po jeho 3D tlač. Článok hodnotí možnosti manipulácie s týmto 3D záznamom a tiež sa detailnejšie sa venuje téme „rapid prototypingu" a perspektívam klinického využitia rôznych variant 3D tlače vrátane tlače biokompatibilnými materiálmi, napríklad titánom (AM/ EMB).
The article introduces and reviews revolutionary technologies and procedures in orthodontics: true ar pseudo 3D record, its processing and visualization. In particular, it describes procedure from CBCT iCATTM scanning through its export to stereolithographic (STL) format and final 3D print. In addition, the article reviews possibilities of 3D CBCT volume and brings the current view on rapid prototyping and perspectives of various 3D print techniques in clinical orthodontics including printing by biocompatible materials like titanium (AM/EBM).
The integration of 3D printing into the pharmaceutical sciences opens new possibilities for personalized medicine. Poly(lactide) (PLA), a biodegradable and biocompatible polymer, is highly suitable for biomedical applications, particularly in the context of 3D printing. However, its processability often requires the addition of plasticizers. This study investigates the use of phase diagram modeling as a tool to guide the rational selection of plasticizers and to assess their impact on the thermodynamic and kinetic stability of PLA-based amorphous solid dispersions (ASDs) containing active pharmaceutical ingredients (APIs). Thermodynamic stability against API recrystallization was predicted based on the API solubility in PLA and Plasticizer-PLA carriers using the Conductor-like Screening Model for Real Solvents (COSMO-RS), while the kinetic stability of the ASDs was evaluated by modeling the glass transition temperatures of the mixtures. Two APIs, indomethacin (IND) and naproxen (NAP), with differing glass-forming abilities (i.e., recrystallization tendencies), and three plasticizers, triacetin (TA), triethyl citrate (TEC), and poly(L-lactide-co-caprolactone) (PLCL), were selected for investigation. The physical stability of ASD formulations containing 9 wt% API and plasticizer to PLA in two ratios, 10:81 and 20:71 w/w %, was monitored over time using differential scanning calorimetry and X-ray powder diffraction and compared with phase diagram predictions. All formulations were predicted to be thermodynamically unstable; however, those containing no plasticizer or with TEC and TA at 10 wt% were predicted to exhibit some degree of kinetic stability. Long-term physical studies corroborated these predictions. The correlation between the predicted phase behavior and long-term physical stability highlights the potential of phase diagram modeling as a tool for the rational design of ASDs in pharmaceutical 3D printing.
- MeSH
- 3D tisk * MeSH
- citráty chemie MeSH
- diferenciální skenovací kalorimetrie metody MeSH
- farmaceutická chemie metody MeSH
- farmaceutická technologie metody MeSH
- indomethacin * chemie MeSH
- krystalizace MeSH
- naproxen chemie MeSH
- polyestery * chemie MeSH
- rozpouštědla chemie MeSH
- rozpustnost * MeSH
- stabilita léku MeSH
- termodynamika MeSH
- tranzitní teplota MeSH
- triacetin chemie MeSH
- změkčovadla * chemie MeSH
- Publikační typ
- časopisecké články MeSH
'Bioinks' are important tools for the fabrication of artificial living-tissue constructs that are able to mimic all properties of native tissues via 3D bioprinting technologies. Bioinks are most commonly made by incorporating live cells of interest within a natural or synthetic biocompatible polymeric matrix. In oncology research, the ability to recreate a tumor microenvironment (TME) using by 3D bioprinting constitutes a promising approach for drug development, screening, and in vitro cancer modeling. Here, we review the different types of bioink used for 3D bioprinting, with a focus on its application in cancer management. In addition, we consider the fabrication of bioink using customized materials/cells and their properties in the field of cancer drug discovery.
- MeSH
- 3D tisk * MeSH
- bioprinting * MeSH
- lidé MeSH
- nádory farmakoterapie MeSH
- objevování léků * MeSH
- protinádorové látky terapeutické užití MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- přehledy MeSH
PURPOSE OF REVIEW: This review describes the latest advances in cell therapy, biomaterials and 3D bioprinting for the treatment of cardiovascular disease. RECENT FINDINGS: Cell therapies offer the greatest benefit for patients suffering from chronic ischemic and nonischemic cardiomyopathy. Rather than replacing lost cardiomyocytes, the effects of most cell therapies are mediated by paracrine signalling, mainly through the induction of angiogenesis and immunomodulation. Cell preconditioning, or genetic modifications are being studied to improve the outcomes. Biomaterials offer stand-alone benefits such as bioactive cues for cell survival, proliferation and differentiation, induction of vascularization or prevention of further cardiomyocyte death. They also provide mechanical support or electroconductivity, and can be used to deliver cells, growth factors or drugs to the injured site. Apart from classical biomaterial manufacturing techniques, 3D bioprinting offers greater spatial control over biomaterial deposition and higher resolution of the details, including hollow vessel-like structures. SUMMARY: Cell therapy induces mainly angiogenesis and immunomodulation. The ability to induce direct cardiomyocyte regeneration to replace the lost cardiomyocytes is, however, still missing until embryonic or induced pluripotent stem cell use becomes available. Cell therapy would benefit from combinatorial use with biomaterials, as these can prolong cell retention and survival, offer additional mechanical support and provide inherent bioactive cues. Biomaterials can also be used to deliver growth factors, drugs, and other molecules. 3D bioprinting is a high-resolution technique that has great potential in cardiac therapy.
- MeSH
- 3D tisk * MeSH
- biokompatibilní materiály MeSH
- bioprinting * MeSH
- kardiomyocyty MeSH
- lidé MeSH
- myokard MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- přehledy MeSH
Additive Manufacturing (AM) is a name of a group of technologies that build 3D objects by adding layer-upon-layer of material. There are many technologies, including Rapid Prototyping (RP), Direct Digital Manufacturing (DDM), layered manufacturing and additive fabrication. Many types of materials can be used for AM technology. Biodegradable polymers such as polylactic acid (PLA) and polyhydroxybutyrate (PHB), are currently the subject of intensive research in the field of additive manufacturing and regenerative medicine. A number of biodegradable and bioresorbable materials, as well as scaffold designs, have been experimentally and clinically studied in many research facilities around the world. For effective using of bioprinting technologies in tissue and biomedical engineering, the knowledge of material and technological parameters in the process of printing is necessary. In this study the 3D printer Bioplotter EnvisionTEC (the printer with ability to print different materials from hydrogel to plastic materials) was used. Scaffolds for the purpose of the experiment were prepared via extrusion-based bioprinting. Experimental part of this study was focused on defining the influence of printing parameters and technological pre-processing of the material on quality and mechanical and geometrical properties of printed parts. Testing of printed samples showed high influence of pre-processing of material, mainly drying process, on mechanical and geometric quality of samples. Drying of material before printing process makes the material more stable and allows it to maintain defined material properties for a longer time than non-dried material. Time of heating of the material in printing cartridge has also high impact on material behaviour. Test results showed that if the time of heating of the material in the high temperature cartridge exceeds defined time limit, the material starts to degrade and is no more usable.
- MeSH
- 3D tisk * MeSH
- biokompatibilní materiály * chemie MeSH
- biomedicínské technologie MeSH
- kyselina polyglykolová chemie MeSH
- lidé MeSH
- polymery * MeSH
- pružnost MeSH
- testování materiálů MeSH
- tkáňové inženýrství * MeSH
- tkáňové podpůrné struktury MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- práce podpořená grantem MeSH
UNLABELLED: The aim of this study was to create a 3D printing material with bioactive properties that potentially could be used for a transparent removable orthodontic appliance. MATERIALS AND METHODS: To acrylic monomers, four bioactive glasses at 10% concentration were added, which release Ca, P, Si and F ions. The materials were printed on a 3D printer and tested for flexural strength (24 h and 30 days), sorption and solubility (7 days), ion release to artificial saliva pH = 4 and 7 (42 days) and cytotoxicity in the human fibroblast model. The released ions were determined by plasma spectrometry (Ca, P and Si ions) and ion-selective electrode (F measurement)s. RESULTS: The material obtained released Ca2+ and PO43- ions for a period of 42 days when using glass Biomin C at pH 4. The flexural strength depended on the direction in which the sample was printed relative to the 3D printer platform. Vertically printed samples had a resistance greater than 20%. The 10% Biomin C samples post-cured for 30 min with light had a survival rate of the cells after 72 h of 85%. CONCLUSIONS: Material for 3D printing with bioactive glass in its composition, which releases ions, can be used in the production of orthodontic aligners.
- Publikační typ
- časopisecké články MeSH
Complex in vitro characterization of a blended material based on Poly(Lactic Acid), Poly(Hydroxybutyrate), and Thermoplastic Starch (PLA/PHB/TPS) was performed in order to evaluate its potential for application in the field of tissue engineering. We focused on the biological behavior of the material as well as its mechanical and morphological properties. We also focused on the potential of the blend to be processed by the 3D printer which would allow the fabrication of the custom-made scaffold. Several blends recipes were prepared and characterized. This material was then studied in the context of scaffold fabrication. Scaffold porosity, wettability, and cell-scaffold interaction were evaluated as well. MTT test and the direct contact cytotoxicity test were applied in order to evaluate the toxic potential of the blended material. Biocompatibility studies were performed on the human chondrocytes. According to our results, we assume that material had no toxic effect on the cell culture and therefore could be considered as biocompatible. Moreover, PLA/PHB/TPS blend is applicable for 3D printing. Printed scaffolds had highly porous morphology and were able to absorb water as well. In addition, cells could adhere and proliferate on the scaffold surface. We conclude that this blend has potential for scaffold engineering.
- MeSH
- 3D tisk MeSH
- hydroxybutyráty farmakologie terapeutické užití MeSH
- lidé MeSH
- polyestery farmakologie terapeutické užití MeSH
- tkáňové inženýrství metody MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
