bioelectronics
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- MeSH
- biosenzitivní techniky MeSH
- biotechnologie MeSH
- lékařská elektronika MeSH
- Publikační typ
- periodika MeSH
- Konspekt
- Biologické vědy
- NLK Obory
- biologie
- biochemie
- biomedicínské inženýrství
Biological rhythms pervade physiology and pathophysiology across multiple timescales. Because of the limited sensing and algorithm capabilities of neuromodulation device technology to-date, insight into the influence of these rhythms on the efficacy of bioelectronic medicine has been infeasible. As the development of new devices begins to mitigate previous technology limitations, we propose that future devices should integrate chronobiological considerations in their control structures to maximize the benefits of neuromodulation therapy. We motivate this proposition with preliminary longitudinal data recorded from patients with Parkinson's disease and epilepsy during deep brain stimulation therapy, where periodic symptom biomarkers are synchronized to sub-daily, daily, and longer timescale rhythms. We suggest a physiological control structure for future bioelectronic devices that incorporates time-based adaptation of stimulation control, locked to patient-specific biological rhythms, as an adjunct to classical control methods and illustrate the concept with initial results from three of our recent case studies using chronotherapy-enabled prototypes.
- Publikační typ
- časopisecké články MeSH
- přehledy MeSH
Wastewater samples from a Swedish chemi-thermo-mechanical pulp (CTMP) mill collected at different purification stages in a wastewater treatment plant (WWTP) were analyzed with an amperometric enzyme-based biosensor array in a flow-injection system. In order to resolve the complex composition of the wastewater, the array consists of several sensing elements which yield a multidimensional response. We used principal component analysis (PCA) to decompose the array's responses, and found that wastewater with different degrees of pollution can be differentiated. With the help of partial least squares regression (PLS-R), we could link the sensor responses to the Microtox® toxicity parameter, as well as to global organic pollution parameters (COD, BOD, and TOC). From investigating the influences of individual sensors in the array, it was found that the best models were in most cases obtained when all sensors in the array were included in the PLS-R model. We find that fast simultaneous determination of several global environmental parameters characterizing wastewaters is possible with this kind of biosensor array, in particular because of the link between the sensor responses and the biological effect onto the ecosystem into which the wastewater would be released. In conjunction with multivariate data analysis tools, there is strong potential to reduce the total time until a result is yielded from days to a few minutes.
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- MeSH
- bioinženýrství MeSH
- terapie metody MeSH
- výpočetní biologie * MeSH
- Publikační typ
- periodika MeSH
- Konspekt
- Biotechnologie. Genetické inženýrství
- NLK Obory
- biomedicínské inženýrství
Bio-elektro-medicína (Bioelectronics) je obor, do kterého nyní vkládají velké naděje už i komerční firmy jako např. Google, který skrze svou dceřinou společnost Alphabet spojil své síly s GlaxoSmithKline (GSK) na vytvoření nové společnosti Galvani Bioelectronics, do které plánují investovat v příštích 7 letech 715 milionů dolarů. GSK věří, že pomocí elektrických signálů by šlo léčit nemoci jako diabetes, artritidu, astma (zdroj: Reuters).
Clinical obstetrics and gynecology
S.545-781 (545-679, 681-781) : obr., tab., přeruš.bibliogr.
Combining the unique properties of peptides as versatile tools for nano- and biotechnology with lead halide perovskite nanoparticles can bring exceptional opportunities for the development of optoelectronics, photonics, and bioelectronics. As a first step towards this challenge sub 10 nm methylammonium lead bromide perovskite colloidal nanoparticles have been synthetizes using commercial cyclic peptide Cyclo(RGDFK), containing 5 amino acids, as a surface stabilizer. Perovskite nanoparticles passivated with Cyclo(RGDFK) possess charge transfer from the perovskite core to the peptide shell, resulting in lower photoluminescence quantum yields, which however opens a path for the application where charge transfer is favorable.
This paper reports on the synthesis and characterization of a novel electrochemical label for sensitive electrochemical stripping metalloimmunoassays based on silver dendrimer-encapsulated nanoparticles (NPs). Silver dendrimer nanocomposites (Ag-DNCs) were synthesized from a generation 5-7 (G5-7) hydroxyl-terminated ethylenediamine-core-type (2-carbon core) PAMAM dendrimer. Several fixed ratios of Ag(+)/dendrimer were prepared with the aim to obtain stable nanocomposites with maximal silver loading in the interior of a polymeric shell. Synthesized Ag-DNCs were characterized by UV-vis spectrophotometry, atomic force microscopy (AFM), transmission electron microscopy (TEM) and X-ray photoelectron spectroscopy (XPS). The G7 Ag-DNC synthesized in 2000 molar excess of silver (1/4 ratio of tertiary amine/Ag(+)) turned out a more suitable candidate for the label development. By combination of the differential pulse voltammetry (DPV) and the anodic stripping analysis (ASV) on a carbon electrode, down to 1.35 x 10(+10) of individual Ag-DNCs (LOD=0.9 pM, 25 ml volume) was detected after the dissolution of silver nanoparticles in a diluted nitric acid. The potential advantages of proposed electrochemical label are discussed.
- MeSH
- analýza selhání vybavení MeSH
- barvení a značení metody MeSH
- biokompatibilní potahované materiály chemie MeSH
- biosenzitivní techniky přístrojové vybavení MeSH
- dendrimery chemie MeSH
- design vybavení MeSH
- elektrochemie přístrojové vybavení MeSH
- financování organizované MeSH
- imunoanalýza přístrojové vybavení MeSH
- nanočástice chemie ultrastruktura MeSH
- nanotechnologie metody přístrojové vybavení MeSH
- reprodukovatelnost výsledků MeSH
- senzitivita a specificita MeSH
- stříbro chemie MeSH
High-throughput surface plasmon resonance (SPR) biosensor for rapid and parallelized detection of nucleic acids identifying specific bacterial pathogens is reported. The biosensor consists of a high-performance SPR imaging sensor with polarization contrast and internal referencing (refractive index resolution 2 x 10(-7) RIU) and an array of DNA probes microspotted on the surface of the SPR sensor. It is demonstrated that short sequences of nucleic acids (20-23 bases) characteristic for bacterial pathogens such as Brucella abortus, Escherichia coli, and Staphylococcus aureus can be detected at 100 pM levels. Detection of specific DNA or RNA sequences can be performed in less than 15 min by the reported SPR sensor.
- MeSH
- aerobní bakterie genetika izolace a purifikace MeSH
- analýza potravin metody MeSH
- analýza selhání vybavení MeSH
- biosenzitivní techniky metody přístrojové vybavení MeSH
- design vybavení MeSH
- DNA bakterií analýza genetika MeSH
- financování organizované MeSH
- kontaminace potravin analýza MeSH
- potravinářská mikrobiologie MeSH
- povrchová plasmonová rezonance metody přístrojové vybavení MeSH
- reprodukovatelnost výsledků MeSH
- sekvenční analýza hybridizací s uspořádaným souborem oligonukleotidů přístrojové vybavení MeSH
- senzitivita a specificita MeSH
- Publikační typ
- hodnotící studie MeSH
This paper describes the direct label-free detection of antibodies against the Epstein-Barr virus (anti-EBNA) using a surface plasmon resonance (SPR) biosensor. The antibody detection was performed using the immunoreaction between anti-EBNA and a respective synthetic peptide (EBNA-1), which was conjugated with bovine serum albumin (BSA-EBNA) and immobilized on the sensor surface. Three immobilization chemistries for the attachment of BSA-EBNA were investigated to optimize ligand density and minimize loss of EBNA-1 immunoreactivity. The developed SPR biosensor functionalized with the optimal immobilization method was calibrated and characterized in terms of detection limit, reproducibility, regenerability and storability. It was demonstrated that the sensor is capable of detecting concentrations of anti-EBNA as low as 0.2 ng/ml (approximately 1 pM) both in buffer and 1% human serum and can be stored and regenerated for repeated use.
- MeSH
- analýza selhání vybavení MeSH
- biosenzitivní techniky metody přístrojové vybavení MeSH
- design vybavení MeSH
- financování organizované MeSH
- imunoanalýza metody přístrojové vybavení MeSH
- protilátky analýza imunologie MeSH
- virus Epsteinův-Barrové - jaderné antigeny analýza imunologie MeSH
- virus Epsteinův-Barrové imunologie MeSH
